甘蔗割手密种类甜蛋白家族鉴定及栽培种同源基因功能分析

苏亚春, 李聪娜, 苏炜华, 尤垂淮, 岑光莉, 张畅, 任永娟, 阙友雄

Identification of thaumatin-like protein family in Saccharum spontaneum and functional analysis of its homologous gene in sugarcane cultivar

SU Ya-Chun, LI Cong-Na, SU Wei-Hua, YOU Chui-Huai, CEN Guang-Li, ZHANG Chang, REN Yong-Juan, QUE You-Xiong

表1 SsTLP家族蛋白的理化性质分析及亚细胞定位和信号肽预测

Table 1 Analysis of physicochemical properties, subcellular localizations, and signal peptides of SsTLP family proteins

基因名称 基因ID 氨基酸数目 分子量 等电点 不稳定系数 平均疏水性 亚细胞定位 信号肽 Gene name Gene ID Amino acids number Molecular weight (kD) Isoelectric point Instability index Hydrophobicity Subcellular localization Signal peptide SsTLP1 Sspon.01G0006490-1A 294 29.00 4.81 27.25 0.13 细胞质Cytoplasm 无No SsTLP2 Sspon.01G0006490-2C 324 32.03 5.59 29.88 0.24 细胞质Cytoplasm 有Yes SsTLP3 Sspon.01G0006490-3D 325 32.05 5.23 30.42 0.26 细胞质Cytoplasm 有Yes SsTLP4 Sspon.01G0016190-1A 625 64.44 5.20 56.53 -0.10 细胞质Cytoplasm 无No SsTLP5 Sspon.01G0016190-2B 464 46.16 5.02 59.63 0.10 细胞膜, 细胞质Cell membrane, Cytoplasm 无No SsTLP6 Sspon.01G0016190-3C 479 47.69 4.96 59.17 0.10 细胞膜, 细胞质Cell membrane, Cytoplasm 无No SsTLP7 Sspon.01G0016190-4D 275 27.67 4.70 54.56 0.16 细胞质Cytoplasm 有Yes SsTLP8 Sspon.01G0016220-1A 331 32.82 5.40 41.00 0.24 细胞质Cytoplasm 有Yes SsTLP9 Sspon.01G0016220-2C 305 31.16 8.33 59.42 0.08 细胞质Cytoplasm 有Yes SsTLP10 Sspon.01G0016220-3D 331 32.82 5.37 40.32 0.25 细胞质Cytoplasm 有Yes SsTLP11 Sspon.01G0018320-1A 236 23.36 4.30 32.97 0.18 细胞质Cytoplasm 有Yes SsTLP12 Sspon.01G0018320-2B 236 23.40 4.30 31.18 0.20 细胞质Cytoplasm 有Yes SsTLP13 Sspon.01G0018320-3C 236 23.41 4.30 34.15 0.15 细胞质Cytoplasm 有Yes SsTLP14 Sspon.01G0020410-2C 1053 113.01 6.79 45.32 -0.14 细胞核Nucleus 无No SsTLP15 Sspon.01G0020420-1A 297 30.21 5.60 47.32 0.08 细胞质Cytoplasm 无No SsTLP16 Sspon.01G0020420-2B 298 30.25 5.40 45.49 0.10 细胞质Cytoplasm 无No SsTLP17 Sspon.01G0037110-1B 334 33.68 5.03 46.39 0.06 细胞质Cytoplasm 有Yes SsTLP18 Sspon.01G0037110-1T 334 33.68 5.03 46.39 0.06 细胞质Cytoplasm 有Yes SsTLP19 Sspon.01G0037110-2C 334 33.68 5.03 46.39 0.06 细胞质Cytoplasm 有Yes SsTLP20 Sspon.01G0043210-1B 289 29.55 7.44 40.68 0.12 细胞质Cytoplasm 有Yes SsTLP21 Sspon.01G0043210-2C 424 44.00 9.26 51.46 -0.17 细胞质Cytoplasm 有Yes SsTLP22 Sspon.01G0043210-3D 292 29.81 7.44 41.03 0.12 细胞质Cytoplasm 有Yes SsTLP23 Sspon.01G0044270-1B 109 11.37 8.28 71.74 -0.05 细胞质Cytoplasm 无No SsTLP24 Sspon.01G0056200-1C 95 10.02 8.89 73.11 -0.62 细胞质Cytoplasm 无No SsTLP25 Sspon.01G0056200-2D 144 14.95 6.51 44.39 -0.24 细胞质Cytoplasm 无No SsTLP26 Sspon.02G0009140-1A 246 24.96 4.87 43.80 -0.16 细胞质Cytoplasm 无No SsTLP27 Sspon.02G0009140-1P 326 34.51 6.21 46.96 -0.22 细胞质Cytoplasm 无No SsTLP28 Sspon.02G0009140-2B 236 23.98 4.87 43.62 -0.12 细胞质Cytoplasm 无No SsTLP29 Sspon.02G0009140-2P 308 32.49 4.99 43.67 -0.10 细胞质Cytoplasm 无No SsTLP30 Sspon.02G0009140-3C 236 23.97 4.87 44.87 -0.13 细胞质Cytoplasm 无No SsTLP31 Sspon.02G0009140-3P 316 33.34 5.36 45.83 -0.15 细胞质Cytoplasm 有Yes SsTLP32 Sspon.02G0009140-4D 319 33.65 5.36 45.49 -0.13 细胞质Cytoplasm 有Yes SsTLP33 Sspon.02G0009150-1A 328 32.84 4.91 47.87 0.16 细胞质Cytoplasm 有Yes SsTLP34 Sspon.02G0009150-2D 318 31.83 4.79 46.30 0.10 细胞质Cytoplasm 无No SsTLP35 Sspon.02G0011230-1A 323 34.14 6.06 43.42 0.00 细胞质Cytoplasm 有Yes SsTLP36 Sspon.02G0011230-1P 220 22.52 4.44 61.16 -0.17 细胞质Cytoplasm 无No SsTLP37 Sspon.02G0011230-2B 289 30.43 7.36 44.32 -0.11 细胞质Cytoplasm 无No SsTLP38 Sspon.02G0011230-2P 231 23.89 4.87 43.93 0.03 细胞质Cytoplasm 有Yes SsTLP39 Sspon.02G0011230-3C 382 38.73 4.45 51.65 -0.12 细胞质Cytoplasm 有Yes SsTLP40 Sspon.02G0011230-4D 323 34.23 6.18 42.60 0.00 细胞质Cytoplasm 有Yes SsTLP41 Sspon.02G0020000-1A 137 14.35 8.32 61.73 -0.36 细胞质Cytoplasm 无No SsTLP42 Sspon.02G0020000-1P 288 29.63 7.39 48.63 -0.12 细胞质Cytoplasm 有Yes SsTLP43 Sspon.02G0029050-1A 135 13.93 6.52 46.66 0.35 细胞质Cytoplasm 有Yes SsTLP44 Sspon.02G0029050-2B 227 23.17 4.74 39.91 0.25 细胞质Cytoplasm 有Yes SsTLP45 Sspon.02G0029050-3C 227 23.23 4.78 39.64 0.26 细胞质Cytoplasm 有Yes SsTLP46 Sspon.02G0029050-4D 215 21.88 4.96 40.74 0.39 细胞质Cytoplasm 有Yes SsTLP47 Sspon.02G0029070-1A 190 19.81 11.44 56.10 -0.22 细胞壁, 细胞质Cell wall, Cytoplasm 无No SsTLP48 Sspon.02G0029080-1A 459 47.73 8.61 60.12 -0.25 细胞质Cytoplasm 无No SsTLP49 Sspon.02G0032190-1A 163 17.00 5.76 46.52 -0.29 细胞壁, 细胞质Cell wall, Cytoplasm 无No SsTLP50 Sspon.02G0032190-1P 170 17.33 4.94 40.68 0.02 细胞质Cytoplasm 有Yes SsTLP51 Sspon.02G0032190-2B 168 17.20 5.54 42.99 0.04 细胞质Cytoplasm 有Yes SsTLP52 Sspon.02G0032190-3C 163 17.02 5.52 45.34 -0.34 细胞壁, 细胞质Cell wall, Cytoplasm 无No SsTLP53 Sspon.02G0032190-4D 168 17.20 5.54 42.99 0.04 细胞质Cytoplasm 有Yes SsTLP54 Sspon.02G0032200-1A 179 18.78 9.85 50.54 0.04 细胞质Cytoplasm 有Yes SsTLP55 Sspon.02G0032220-1A 171 17.61 4.68 29.57 -0.06 细胞质Cytoplasm 有Yes SsTLP56 Sspon.02G0032220-2B 171 17.38 4.35 44.70 0.22 细胞质Cytoplasm 有Yes SsTLP57 Sspon.02G0032220-3C 132 13.56 4.12 39.94 0.09 细胞质Cytoplasm 有Yes SsTLP58 Sspon.02G0032230-1A 168 17.20 5.54 42.99 0.04 细胞质Cytoplasm 有Yes SsTLP59 Sspon.02G0032230-2C 409 43.36 8.98 48.87 -0.31 细胞质, 细胞核Cytoplasm, Nucleus 有Yes SsTLP60 Sspon.02G0032240-1A 201 20.71 6.38 26.29 -0.09 细胞质Cytoplasm 有Yes SsTLP61 Sspon.02G0032240-2C 175 17.72 4.60 43.06 0.09 细胞质Cytoplasm 有Yes SsTLP62 Sspon.02G0034650-1B 230 24.19 7.31 24.08 -0.01 细胞质Cytoplasm 有Yes SsTLP63 Sspon.02G0034650-2D 224 23.68 7.30 22.53 0.02 细胞质Cytoplasm 有Yes SsTLP64 Sspon.02G0036340-1B 317 32.21 4.51 39.86 0.12 细胞质Cytoplasm 无No SsTLP65 Sspon.02G0036340-2C 322 32.67 4.51 38.40 0.13 细胞质Cytoplasm 有Yes SsTLP66 Sspon.02G0036340-3D 316 32.12 4.51 39.95 0.12 细胞质Cytoplasm 无No SsTLP67 Sspon.02G0041150-1B 291 30.58 8.03 51.00 0.04 细胞质Cytoplasm 有Yes SsTLP68 Sspon.02G0041150-2C 383 40.61 8.39 48.90 -0.01 细胞质Cytoplasm 有Yes SsTLP69 Sspon.02G0045690-2D 171 17.51 4.47 29.08 -0.03 细胞质Cytoplasm 有Yes SsTLP70 Sspon.02G0045700-1B 173 17.67 4.26 31.34 0.10 细胞质Cytoplasm 有Yes SsTLP71 Sspon.02G0045700-1P 416 42.99 9.33 35.28 -0.38 细胞质Cytoplasm 有Yes SsTLP72 Sspon.02G0045700-2C 173 17.68 4.43 31.87 0.14 细胞质Cytoplasm 有Yes SsTLP73 Sspon.02G0045700-3D 173 17.73 4.43 33.23 0.09 细胞质Cytoplasm 有Yes SsTLP74 Sspon.02G0053220-1C 245 25.68 6.27 27.29 -0.32 细胞质Cytoplasm 有Yes SsTLP75 Sspon.02G0053220-2D 271 28.16 5.87 38.49 -0.29 细胞质Cytoplasm 有Yes SsTLP76 Sspon.02G0053240-1C 166 17.35 6.66 30.90 -0.17 细胞质Cytoplasm 有Yes SsTLP77 Sspon.02G0053250-2D 233 24.46 8.92 60.54 -0.45 细胞质, 细胞核Cytoplasm, Nucleus 无No SsTLP78 Sspon.02G0059040-1D 124 12.61 6.50 44.52 0.23 细胞膜, 细胞质Cell membrane, Cytoplasm 有Yes SsTLP79 Sspon.02G0059050-1D 127 12.95 4.27 27.47 0.47 细胞质Cytoplasm 有Yes SsTLP80 Sspon.03G0003060-1A 252 26.62 7.86 47.70 -0.05 细胞质Cytoplasm 有Yes SsTLP81 Sspon.03G0003060-1P 253 26.73 7.86 47.55 -0.04 细胞质Cytoplasm 有Yes SsTLP82 Sspon.03G0003060-2C 251 26.48 7.86 46.89 -0.06 细胞质Cytoplasm 有Yes SsTLP83 Sspon.03G0014170-1A 633 69.61 6.63 48.05 -0.21 细胞核Nucleus 有Yes SsTLP84 Sspon.03G0018890-1A 1028 111.78 9.42 52.82 -0.72 细胞核Nucleus 无No SsTLP85 Sspon.03G0018890-2C 516 57.24 6.15 38.71 -0.30 细胞核Nucleus 无No SsTLP86 Sspon.03G0018890-3D 727 80.10 6.23 49.20 -0.33 细胞核Nucleus 有Yes SsTLP87 Sspon.03G0040280-1C 227 23.49 7.33 31.85 -0.08 细胞质Cytoplasm 有Yes SsTLP88 Sspon.03G0040280-1P 227 23.51 7.33 31.48 -0.07 细胞质Cytoplasm 有Yes SsTLP89 Sspon.04G0003480-1A 186 18.49 4.09 48.17 0.20 细胞质Cytoplasm 无No SsTLP90 Sspon.04G0003480-2C 186 18.49 4.09 48.17 0.20 细胞质Cytoplasm 无No SsTLP91 Sspon.04G0003480-3D 191 18.97 4.41 47.57 0.21 细胞质Cytoplasm 无No SsTLP92 Sspon.05G0007890-1A 1136 124.24 5.26 52.74 -0.44 细胞核Nucleus 无No SsTLP93 Sspon.05G0014480-1A 280 27.85 5.42 47.70 0.28 细胞质Cytoplasm 有Yes SsTLP94 Sspon.05G0014480-1P 267 26.55 5.68 46.13 0.23 细胞质Cytoplasm 有Yes SsTLP95 Sspon.05G0014480-2B 280 27.78 5.35 45.12 0.34 细胞质Cytoplasm 有Yes SsTLP96 Sspon.05G0014480-3D 277 27.62 5.35 44.31 0.32 细胞质Cytoplasm 有Yes SsTLP97 Sspon.05G0029070-1B 550 60.87 6.45 43.06 -0.32 细胞核Nucleus 有Yes SsTLP98 Sspon.05G0029070-2C 514 56.87 6.33 45.88 -0.35 细胞核Nucleus 有Yes SsTLP99 Sspon.05G0032300-1C 244 24.70 4.62 36.67 0.09 细胞质Cytoplasm 有Yes SsTLP100 Sspon.05G0032300-2D 392 40.73 7.03 46.75 -0.08 细胞质Cytoplasm 无No SsTLP101 Sspon.05G0032310-1C 242 25.22 4.53 47.73 -0.18 细胞质Cytoplasm 无No SsTLP102 Sspon.05G0032310-2D 242 25.19 4.53 47.73 -0.17 细胞质Cytoplasm 无No SsTLP103 Sspon.06G0027890-1B 241 25.13 6.50 41.80 -0.14 细胞质Cytoplasm 无No SsTLP104 Sspon.06G0027890-1T 217 22.57 8.36 44.97 -0.11 细胞质Cytoplasm 无No SsTLP105 Sspon.06G0027890-2C 251 26.21 8.10 39.51 -0.11 细胞质Cytoplasm 无No SsTLP106 Sspon.07G0015550-1A 318 32.05 4.60 49.86 0.08 细胞质Cytoplasm 有Yes SsTLP107 Sspon.07G0015550-2B 326 33.01 7.35 38.08 0.30 细胞质Cytoplasm 有Yes SsTLP108 Sspon.07G0015560-1A 517 54.47 4.77 46.95 -0.11 细胞质, 细胞核, 液泡Cytoplasm, Nucleus, Vacuole 有Yes SsTLP109 Sspon.07G0017710-1A 267 26.60 4.10 43.87 0.22 细胞质Cytoplasm 有Yes SsTLP110 Sspon.07G0017710-2B 329 32.60 4.21 47.78 0.28 细胞膜, 细胞质, 液泡Cell membrane, Cytoplasm, Vacuole 有Yes SsTLP111 Sspon.08G0001280-1A 260 25.63 4.82 46.65 0.35 细胞质Cytoplasm 有Yes SsTLP112 Sspon.08G0001280-2C 260 25.58 4.65 46.15 0.36 细胞质Cytoplasm 有Yes SsTLP113 Sspon.08G0001280-3D 279 27.61 4.92 47.72 0.10 细胞质Cytoplasm 无No SsTLP114 Sspon.08G0003010-1A 248 25.25 8.59 38.06 0.13 细胞质Cytoplasm 有Yes SsTLP115 Sspon.08G0003010-2B 254 25.87 8.59 36.97 0.14 细胞质Cytoplasm 有Yes SsTLP116 Sspon.08G0003010-3C 248 25.26 8.59 37.63 0.15 细胞质Cytoplasm 有Yes SsTLP117 Sspon.08G0007410-1A 313 31.28 4.74 33.53 0.25 细胞质Cytoplasm 有Yes SsTLP118 Sspon.08G0007410-2C 313 31.25 4.74 34.28 0.24 细胞质Cytoplasm 有Yes SsTLP119 Sspon.08G0015670-1A 309 31.95 6.13 49.19 0.07 细胞质Cytoplasm 有Yes SsTLP120 Sspon.08G0015670-2B 309 31.92 6.20 46.42 0.07 细胞质Cytoplasm 有Yes SsTLP121 Sspon.08G0015670-3D 251 26.04 9.12 55.78 -0.23 细胞质Cytoplasm 有Yes SsTLP122 Sspon.08G0030460-1D 275 28.40 6.12 54.61 0.10 细胞质Cytoplasm 有Yes