甘蔗割手密种类甜蛋白家族鉴定及栽培种同源基因功能分析

苏亚春, 李聪娜, 苏炜华, 尤垂淮, 岑光莉, 张畅, 任永娟, 阙友雄

Identification of thaumatin-like protein family in Saccharum spontaneum and functional analysis of its homologous gene in sugarcane cultivar

SU Ya-Chun, LI Cong-Na, SU Wei-Hua, YOU Chui-Huai, CEN Guang-Li, ZHANG Chang, REN Yong-Juan, QUE You-Xiong

图10 甘蔗ScTLP1蛋白的转录自激活活性验证 A: 不同浓度的阳性对照、阴性对照和pGBKT7-ScTLP1的Y2H Gold酵母菌液涂布在SD-Trp固体培养基上的生长情况。B: 利用X-α-gal染色液对SD-Trp固体培养基上的菌落的染色结果。C: X-α-gal染色液对添加了金担子素A抑制剂SD-Trp固体培养基上生长的菌落的染色结果。pGADT7-T+pGBKT7-p53: 阳性对照; pGBKT7: 阴性对照。

Fig. 10 Validation of transcriptional self-activation activity of ScTLP1 protein A: the growth of Y2H Gold yeast solution sprayed on SD-Trp solid medium with different concentrations of positive control, negative control and pGBKT7-ScTLP1. B: colonial staining result of SD-Trp solid culture medium with X-α-gal staining solution. C: colonial staining result after adding SD-Trp solid culture with X-α-gal staining solution. pGADT7-T+pGBKT7-p53: positive control; pGBKT7: negative control.