甘蔗割手密种类甜蛋白家族鉴定及栽培种同源基因功能分析

苏亚春, 李聪娜, 苏炜华, 尤垂淮, 岑光莉, 张畅, 任永娟, 阙友雄

Identification of thaumatin-like protein family in Saccharum spontaneum and functional analysis of its homologous gene in sugarcane cultivar

SU Ya-Chun, LI Cong-Na, SU Wei-Hua, YOU Chui-Huai, CEN Guang-Li, ZHANG Chang, REN Yong-Juan, QUE You-Xiong

图8 ScTLP1基因在本氏烟叶片中的瞬时表达效应 A: ScTLP1在瞬时表达1 d后的本氏烟中的基因表达量。B: 烟草免疫相关基因在瞬时表达1 d后的对照组和实验组的基因表达量。C: 瞬时表达2 d后的本氏烟叶片的DAB染色结果。D和E: 本氏烟接种烟草青枯菌和烟草茄病镰刀菌蓝色变种7 d的叶片表型。实验数据以NtEF-1α基因的表达水平进行归一化处理。误差线代表每组处理的标准误差(n = 3)。柱上标以不同小写字母表示在0.05水平显著差异。

Fig. 8 Transient expression effect of ScTLP1 gene in Nicotiana benthamiana leaves A: gene expression level of ScTLP1 in N. benthamiana after transient expression for 1 d. B: gene expression level of tobacco immune related genes in control group and experimental group after transient expression for 1 d. C: the DAB staining result of N. benthamiana leaves after transient expression for 2 d. D-E: the leaf phenotypes of N. benthamiana inoculated with Pseudomonas solanacearum and Fusarium solani var. coeruleum for seven days, respectively. The experimental data were normalized by the expression level of NtEF-1α gene. The error bars represent the standard errors of each group treatment (n = 3). Different lowercase letters above the bars mean significant differences at the 0.05 probability level.