基于BSA-Seq和RNA-Seq方法鉴定大豆抗豆卷叶螟候选基因

曾维英, 赖振光, 孙祖东, 杨守臻, 陈怀珠, 唐向民

Identification of the candidate genes of soybean resistance to bean pyralid (Lamprosema indicata Fabricius) by BSA-Seq and RNA-Seq

ZENG Wei-Ying, LAI Zhen-Guang, SUN Zu-Dong, YANG Shou-Zhen, CHEN Huai-Zhu, TANG Xiang-Min

表1 原始测序数据统计表

Table 1 Quality statistics of raw data

样本 Sample 原始序列数 Raw reads 原始碱基数 Raw bases 过滤后序列数 Clean reads 过滤后碱基数 Clean bases 有效率 Clean_data/ raw_data (%) GC含量 Clean_GC_rate (%) Q20含量 Q20 (%) Q30含量Q30 (%) 皖82-178 Wan 82-178 438,210,500 65,731,575,000 432,474,442 64,871,166,300 98.69 35.57 96.50 92.13 赶泰-2-2 Gantai-2-2 441,716,184 66,257,427,600 433,990,902 65,098,635,300 98.25 35.60 96.34 91.79 高感子代池 Highly susceptible pool 206,898,280 31,034,742,000 202,406,834 30,361,025,100 97.83 35.54 96.44 92.01 高抗子代池 Highly resistant pool 210,122,884 31,518,432,600 206,805,618 31,020,842,700 98.42 35.46 96.29 91.70