基于BSA-Seq和RNA-Seq方法鉴定大豆抗豆卷叶螟候选基因

曾维英, 赖振光, 孙祖东, 杨守臻, 陈怀珠, 唐向民

Identification of the candidate genes of soybean resistance to bean pyralid (Lamprosema indicata Fabricius) by BSA-Seq and RNA-Seq

ZENG Wei-Ying, LAI Zhen-Guang, SUN Zu-Dong, YANG Shou-Zhen, CHEN Huai-Zhu, TANG Xiang-Min

表2 质控后测序数据与参考基因组比对结果统计分析

Table 2 Matching of quality control data with reference genome

样本 Sample 过滤后序 列数 Clean reads 比对上数据 Mapping reads 比对率 Mapping rate (%) 优质比对率 Properly paired ratio (%) 平均测序深度 Mean depth 1×覆盖度Coverage ≥ 1× (%) 5×覆盖度 Coverage ≥ 5× (%) 10×覆盖度 Coverage ≥ 10× (%) 20×覆盖度 Coverage ≥ 20× (%) 皖82-178 Wan 82-178 43,4152,986 433,232,376 99.79 99.73 65.78 96.67 96.12 95.67 94.62 赶泰-2-2 Gantai-2-2 435,703,158 434,715,984 99.77 99.72 65.98 96.68 96.13 95.68 94.60 高感子代池 Highly susceptible pool 203,202,650 202,820,220 99.81 99.76 30.78 95.75 94.55 93.29 83.88 高抗子代池 Highly resistant pool 207,33,600 207,143,542 99.76 99.71 31.44 95.76 94.56 93.32 84.44