基于BSA-Seq和RNA-Seq方法鉴定大豆抗豆卷叶螟候选基因

曾维英, 赖振光, 孙祖东, 杨守臻, 陈怀珠, 唐向民

Identification of the candidate genes of soybean resistance to bean pyralid (Lamprosema indicata Fabricius) by BSA-Seq and RNA-Seq

ZENG Wei-Ying, LAI Zhen-Guang, SUN Zu-Dong, YANG Shou-Zhen, CHEN Huai-Zhu, TANG Xiang-Min

表3 多态性位点注释统计表

Table 3 Annotation of polymorphism sites

变异位点信息 Variation sites information 皖82-178 Wan 82-178 赶泰-2-2 Gantai-2-2 高感子代池 Highly susceptible pool 高抗子代池 Highly resistant pool 内含子Intron 324,202 324,333 230,606 231,332 基因区间Intergenic region 2,589,457 2,588,540 1,851,943 1,867,897 可变剪切位点Splicing 612 620 449 444 基因上游Upstream 192,755 192,753 133,797 136,203 基因下游Downstream 162,024 161,574 113,186 114,671 基因上游/基因下游Upstream/downstream 12,038 12,088 8296 8733 5°非翻译区UTR5° 23,658 23,746 68,071 16,818 3°非翻译区UTR3° 29,843 29,595 20,949 21,579 终止子提前Stop gain 1652 1649 1177 1225 终止子丢失Stop loss 270 276 197 204 同义突变Synonymous 48,462 48,433 35,671 35,492 非同义突变Non-synonymous 67,206 67,040 49,333 49,653 SNP总数SNP numbers 3,487,363 3,485,674 2,483,855 2,506,185 纯合突变SNP Hom SNP number 3,480,115 3,478,433 2,477,251 2,499,464 杂合突变SNP Hete SNP number 7248 7241 6604 6721 纯合突变SNP比率Hom SNP rate (%) 99.79 99.79 99.73 99.73 杂合突变SNP比率Het SNP rate (%) 0.21 0.21 0.27 0.27 转换Ts 2,264,913 2,264,110 1,606,918 1,617,797 颠换Tv 1,218,400 1,217,609 872,951 884,443 转换/颠换Ts/Tv 1.86 1.86 1.84 1.83