CRISPR/Cas9编辑花生FAD2基因研究

张旺, 冼俊霖, 孙超, 王春明, 石丽, 于为常

Preliminary study of genome editing of peanut FAD2 genes by CRISPR/Cas9

ZHANG Wang, XIAN Jun-Lin, SUN Chao, WANG Chun-Ming, SHI Li, YU Wei-Chang

表1 FAD2A基因突变

Table 1 Mutation sites of FAD2A gene

突变位点 Mutation site 核酸Nucleic acid 密码子Codon 氨基酸Amino acid 突变前Before mutation 突变后 After mutation 突变前Before mutation 突变后 After mutation 突变前Before mutation 突变后 After mutation 40 A G AAG GAG K E 150 C A TCC TCA 172 G A GTG ATG V M 214 A C AAG CAG K Q 282 T C ACT ACC 333 C T TAC TAT 362 C T ACC ATC T I 384 T C GTT GTC 416 C A CGC CAC R H 419 A T CAC CTC H L 432 C A ACC ACA 448 G A GAC AAC D N 451 G T GAA TAA E 终止密码Stop codon 464, 465 CA TG CCA CTG P L 487 T C TGG CGG W R 515 C T CCA CTA P L 518 G A GGG GAG G E 528 C T ATC ATT 534 C T CTC CTT 668 插入序列7个碱基7 bp insertion (CTCAGGA) 引起移码突变Frame shift mutation 721 C T CTG TTG 800 A G TAT TGT Y C 807 G A CAG CAA 930 G A ACG ACA 963 T A CCT CCA 1017 C T TAC TAT 1039 T G TTT GTT F V 1047 A G AAA AAG 1105 A C AAG CAG K Q