CRISPR/Cas9编辑花生FAD2基因研究

张旺, 冼俊霖, 孙超, 王春明, 石丽, 于为常

Preliminary study of genome editing of peanut FAD2 genes by CRISPR/Cas9

ZHANG Wang, XIAN Jun-Lin, SUN Chao, WANG Chun-Ming, SHI Li, YU Wei-Chang

表2 FAD2B基因突变

Table 2 Mutation sites of FAD2B gene

突变位点 Mutation site 核酸Nucleic acid 密码子Codon 氨基酸Amino acid 突变前 Before mutation 突变后 After mutation 突变前 Before mutation 突变后 After mutation 突变前 Before mutation 突变后 After mutation 40 A G AAG GAG K E 150 A C TCA TCC 172 A G ATG GTG M V 214 A C AAG CAG K Q 282 C T ACC ACT 333 C T TAC TAT 362 C T ACC ATC T I 384 T C GTT GTC 416 C A CGC CAC R H 419 A T CAC CTC H L 432 A C ACA ACC 451 G T GAA TAA E 终止密码Stop codon 464 C T CCG CTG P L 487 T C TGG CGG W R 515 C T CCA CTA P L 518 G A GGG GAG G E 528 T C ATT ATC 534 T C CTT CTC 668 插入序列7个碱基 7 bp insertion (CTCAGGA) 引起移码突变Frame shift mutation 721 T C TTG CTG 800 A G TAT TGT Y C 807 G A CAG CAA 907 G A GCA ACA A T 930 G A ACG ACA 963 T A CCT CCA 982 G T GCA TCA A S 1017 C T TAC TAT 1039 G T GTT TTT V F 1047 A G AAA AAG 1105 C A CAG AAG Q K