CRISPR/Cas9编辑花生FAD2基因研究

张旺, 冼俊霖, 孙超, 王春明, 石丽, 于为常

Preliminary study of genome editing of peanut FAD2 genes by CRISPR/Cas9

ZHANG Wang, XIAN Jun-Lin, SUN Chao, WANG Chun-Ming, SHI Li, YU Wei-Chang

图3 花生基因转化及转基因植株的分析 A: 转基因载体, T1、T2分别为gRNA1和gRNA2靶点序列; B: 转基因愈伤及再生植株, 左侧为抗性愈伤组织, 右侧为转基因植株; C: 转基因植株鉴定, 扩增条带为Cas9基因片段, 箭头指示400 bp PCR扩增产物; D: 突变体序列分析, 转基因植株通过测序检测基因编辑突变体。

Fig. 3 Gene transformation and analysis of transgenic lines in peanut A: gene transformation vector. T1 and T2 are gRNA1 and gRNA2 target sites; B: transgenic calli (left) and plantlets (right); C: PCR identification of transgenic plants. The amplified band is Cas9 gene fragment, and the arrow denotes 400 bp DNA marker; D: sequence analysis of genome editing mutants.