普通小麦-六倍体中间偃麦草易位系的抗条锈鉴定及应用评估

王音, 冯志威, 葛川, 赵佳佳, 乔玲, 武棒棒, 闫素仙, 郑军, 郑兴卫

Identification of seedling resistance to stripe rust in wheat-Thinopyrum intermedium translocation line and its potential application in breeding

WANG Yin, FENG Zhi-Wei, GE Chuan, ZHAO Jia-Jia, QIAO Ling, WU Bang-Bang, YAN Su-Xian, ZHENG Jun, ZHENG Xing-Wei

图2 ZH811 (A, B)和亲本晋麦33 (C, D)、临4133 (E, F)的GISH/FISH分析 A, C, E: 利用中国春基因组做封阻, 中间偃麦草基因组为探针的GISH分析; B, D, F: 利用探针pAs1 (绿色信号)和pSc119.2 (红色信号)进行FISH分析。白色箭头指示小麦-中间偃麦草易位片段(A)和5D染色体(B~F)。标尺为10 μm。

Fig. 2 GISH/FISH analysis of the chromosomes of ZH811 (A, B), Jinmai 33 (C, D), and Lin 4133 (E, F) A, C, E: GISH using genomic DNA of Thinopyrum intermedium as probe, genomic DNA of Chinese Spring as block. B, D, F: FISH using probes pAs1 (green signal) and pSc119.2 (red signal). Arrows show the wheat-Thinopyrum intermedium chromosome translocations (A) or chromosome 5D (B-F). Bar = 10 μm.