甘蔗新品种(系)苗期白条病人工接种抗性鉴定与评价

傅华英, 张婷, 彭文静, 段瑶瑶, 许哲昕, 林艺华, 高三基

Identification of resistance to leaf scald in newly released sugarcane varieties at seedling stage by artificial inoculation

FU Hua-Ying, ZHANG Ting, PENG Wen-Jing, DUAN Yao-Yao, XU Zhe-Xin, LIN Yi-Hua, GAO San-Ji

图2 接种后的病叶白条病菌分离和PCR检测 A: 白色褪绿条纹样品(新台糖22号)分离出来的菌落; B: 白色褪绿条纹样品(闽糖11-610)分离出来的菌落; C: 白化叶片(柳城09-15)出来的菌落; D: 黄化叶片(粤甘49)分离出来的菌落; E: 黄化叶片(福农11-2907)分离出来的菌落; F: 从不同品种分离出来的白条病菌单菌落PCR检测。1: D15000+2000 DNA marker; 2: 空白对照, 无菌水; 3: 阴性对照, 健康植株叶片DNA; 4: 阳性对照, Xa-FJ1菌株; 5~9: 上述分离出来的5个菌株的DNA样品。

Fig. 2 Pathogen isolation and PCR detection of inoculated leaf samples A: colonies isolated from ROC22 with white pencil lines; B: colonies isolated from Mintang 11-610 with white striped leaves; C: colonies isolated from Liucheng 09-15 with chlorotic leaf; D: colonies isolated from Yuegan 49 with yellowing leaf; E: colonies isolated from Funong 11-2907 with yellowing leaf; F: PCR detection for colonies isolated from the above-mentioned samples. 1: D15000+2000 DNA marker; 2: the blank control, sterile H2O; 3: the negative control, healthy leaf sample; 4: the positive control, Xa-FJ1 strain; 5-9: DNA samples from colonies isolated from the above-mentioned samples.