花生AhFAD2-1基因启动子及5'-UTR内含子功能验证及其低温胁迫应答
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石磊, 苗利娟, 黄冰艳, 高伟, 张忠信, 齐飞艳, 刘娟, 董文召, 张新友
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Characterization of the promoter and 5'-UTR intron in AhFAD2-1 genes from peanut and their responses to cold stress
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SHI Lei, MIAO Li-Juan, HUANG Bing-Yan, GAO Wei, ZHANG Zong-Xin, QI Fei-Yan, LIU Juan, DONG Wen-Zhao, ZHANG Xin-You
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表1 本研究所用引物及其序列
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Table 1 Sequences of primers used in this study
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目的片段
Target fragment | 上游引物
Forward primer (5°-3°) | 下游引物
Reverse primer (5°-3°) | | PAhFAD2-1A+intron | ACGCGTCGA (Sal I) CTTCCGCCAGAATGAGGAACTAAA TCACAG | GACATCTAGA (Xba I) GTTGTGTTGTTAAAGCT CCTGTTACCAATG | | PAhFAD2-1B+intron | CGGGGTACC (Kpn I) ATGTTTCATAGAATTTAAGCTCAGA CACG | | PAhFAD2-1B(pseudo) | ACGCGTCGA (Sal I) CACCAAGTAGCTTCTCAATGGCTCA GATTCG | | AhFAD2-1B-intron | ACGCGTCGA (Sal I) CATAGGAGAAGCACTCACTTCTCTT CTCTC | | AhFAD2-1B-intron681 | ACGCGTCGA (Sal I) CATAATGGCTTCTGGGCCCTCAC | | AhFAD2-1B-intron363 | ACGCGTCGA (Sal I) CATAATGGCTTCTGGGCCCTCAC | GACATCTAGA (Xba I) TATCATGCCAAGTGACT AGTATGA | | PAhFAD2-1A | ACGCGTCGA (Sal I) CTTCCGCCAGAATGAGGAACTAAA TCACAG | GACATCTAGA (Xba I) GATGAATCTCGCAGCCA CGTTT | | PAhFAD2-1B | CGGGGTACC (Kpn I) ATGTTTCATAGAATTTAAGCTCAGA CACG |
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