低温胁迫对普通和高油酸花生种子萌发的影响

薛晓梦, 吴洁, 王欣, 白冬梅, 胡美玲, 晏立英, 陈玉宁, 康彦平, 王志慧, 淮东欣, 雷永, 廖伯寿

Effects of cold stress on germination in peanut cultivars with normal and high content of oleic acid

XUE Xiao-Meng, WU JIE, WANG Xin, BAI Dong-Mei, HU Mei-Ling, YAN Li-Ying, CHEN Yu-Ning, KANG Yan-Ping, WANG Zhi-Hui, HUAI Dong-Xin, LEI Yong, LIAO Bo-Shou

表2 Real time PCR引物及其碱基序列

Table 2 Primer sequences of qRT-PCR

基因 Gene 引物名称 Primer name 引物序列 Primer sequence (5°-3°) AhFAD2-1A, AhFAD2-1B RTAhFAD2-1-F ATCTGCTATATCACATAGCAACTCT RTAhFAD2-1-R ACTGTTGCCAATGCTCCTCT AhFAD2-3A, AhFAD2-3B RTAhFAD2-3-F GGTCTTATCCGTCTTGTCATGG RTAhFAD2-3-R AGATGAATCGTAATGTGGCAATG AhFAD2-4A, AhFAD2-4B RTAhFAD2-4-F TCATTCTGCCGGGAAGAGG RTAhFAD2-4-R ATGGCGACATAGGCGAAAAT AhActin Actin-F TAAGAACAATGTTGCCATACAGA Actin-R GTTGCCTTGGATTATGAGC