甘蓝自交不亲和相关基因BoPUB9的克隆及表达分析

谢琴琴, 左同鸿, 胡燈科, 刘倩莹, 张以忠, 张贺翠, 曾文艺, 袁崇墨, 朱利泉

Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea

XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan

表1 本试验所用引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5°-3°) 用途 Functions BoPUB9-AD F: GTACCAGATTACGCTCATATGATGGCAAAGACCGGATTTTTC 酵母双杂交引物 R: ATGCCCACCCGGGTGGAATTCGGCTGTGTGAGTAAGATTCATA Yeast two-hybrid primers SRKj-BK F: GTACCAGATTACGCTCATATGTCAGATCGTGAGGTTTTCAATTC 酵母双杂交引物 R: ATGCCCACCCGGGTGGAATTCGCCTTGTTATCGTTCCATGACTA Yeast two-hybrid primers BoPUB9-PAN F: AAGTCCGGAGCTAGCTCTAGAATGGCAAAGACCGGATTTTTC 基因亚细胞定位 R: GCCCTTGCTCACCATGGATCCGGCTGTGTGAGTAAGATTCATA Subcellular location BoPUB9-PGEX F: GATCTGGTTCCGCGTGGATCCATGGCAAAGACCGGATTTTTC 基因的原核表达 R: GATGCGGCCGCTCGAGTCGACGGCTGTGTGAGTAAGATTCATA Prokaryotic expression BoPUB9-GUS F: CAAGCTTGGCTGCAGGTCGACGTCTTCTTAACGACATGGCTTG 基因的启动子活性分析 R: GGTGGACTCCTCTTAGAATTCCTCCGTCGGATCCACCG Promoter activity analysis dActin F: GGCTGATGGTGAAGATATTCA 内参引物 R: CAAGCACAATACCAGTAGTAC Internal reference primers qRT-PCR F: TGGCTTCTGGTCAGACATACGA 荧光定量PCR引物 R: GTGACAGCTTTCTTGGACTCG qRT-PCR primers