甘蓝自交不亲和相关基因BoPUB9的克隆及表达分析
谢琴琴, 左同鸿, 胡燈科, 刘倩莹, 张以忠, 张贺翠, 曾文艺, 袁崇墨, 朱利泉

Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea
XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan
表2 生物信息学分析所需软件及在线网站
Table 2 Software and online websites for bioinformatics analysis
软件或在线网站
Software and online websites
功能
Functions
Bio-soft (http://www.bio-soft.net/sms/index.html)和DNAMAN 8.0 软件 推导基因编码的氨基酸序列
Bio-soft (http://www.bio-soft.net/sms/index.html) and DNAMAN 8.0 software Derive the amino acid sequence encoded by the gene
ExPASy-ProtParam tool (http://www.expasy.org/) 分析蛋白质理化性质
Analysis of physical and chemical properties of protein
Signalp (http://www.cbs.dtu.dk/services/SignalP/) 预测蛋白质的信号肽
Signal peptides for predicting proteins
TMHMM (http://www.cbs.dtu.dk/services/TMHMM-2.0/) 预测蛋白质的跨膜结构域
Prediction of transmembrane domains of proteins
Netphos (http://www.cbs.dtu.dk/services/NetPhos/) 预测磷酸化位点
Predicted phosphorylation site
NetNGlyc (http://www.cbs.dtu.dk/services/NetNGlyc/) 预测N-糖基化位点
Prediction of N-glycosylation sites
ProtScale (https://web.expasy.org/protscale/) 预测疏水性/亲水性
Prediction of hydrophobicity/hydrophilicity
SMART(http://smart.embl-heidelberg.de/) 蛋白质的高级结构域
Advanced domain of protein
PROSITE (http://prosite.expasy.org/) 蛋白质的功能位点
Functional sites of proteins
PlantCARE (http://bioinformatics.psb.ugentbe/webtools/plantcare/) 分析启动子的顺式作用元件
Analysis of cis-acting elements of promoter
MEGA7软件和NCBI (http://www.ncbi.nlm.nih.gov/) 构建进化树
MEGA7 software and NCBI (http://www.ncbi.nlm.nih.gov/) Construct an evolutionary tree
BRAD (http://brassicadb.org/brad/index.php) 分析基因启动子序列
Analysis of gene promoter sequence