甘蓝自交不亲和相关基因BoPUB9的克隆及表达分析

谢琴琴, 左同鸿, 胡燈科, 刘倩莹, 张以忠, 张贺翠, 曾文艺, 袁崇墨, 朱利泉

Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea

XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan

图10 BoPUB9 蛋白的原核表达 泳道1: 空载pGEX-4T-1; 泳道2: 诱导纯化的pGEX-4T- 1-BoPUB9; 泳道3: 诱导未纯化的的pGEX-4T-1-BoPUB9; 泳道4: 未诱导的pGEX-4T-1-BoPUB9。M: 蛋白分子量标准。

Fig. 10 Prokaryotic expression profile of BoPUB9 protein Lane 1: pGEX-4T-1; Lane 2: induced and purified pGEX-4T-1- BoPUB9; Lane 3: induced non-purified pGEX-4T-1-BoPUB9; Lane 4: non-induced pGEX-4T-1-BoPUB9. M: molecular weight standard of protein.