超低温11年保存期对转基因作物基体标准样品核酸检测的影响

王渭霞, 赖凤香, 胡海燕, 何佳春, 魏琪, 万品俊, 傅强

Effect of 11-year storage of GMO reference material at ultra-low temperature on nucleic acid detection of standard matrix sample of transgenic crop

WANG Wei-Xia, LAI Feng-Xiang, HU Hai-Yan, HE Jia-Chun, WEI Qi, WAN Pin-Jun, FU Qiang

表2 转化体实时荧光PCR对保存9年的转基因含量0.1%的标准物质的检测

Table 2 Detection of reference materials with GM content 0.1% stored for nine years by event-specific qRT-PCR

样品名称 Sample 模板浓度 DNA concentration (mg L-1) Ct值 Ct value 相对标准差 RSD (%) 结论 Result 阳性对照Ct值 Ct value of CK+ Bt11 36.0 36.89±0.53 1.43 阳性Positive 29.32±0.40 Bt176 24.5 34.17±0.09 1.30 阳性Positive 25.39±0.10 MON863 34.5 35.60±0.34 0.95 阳性Positive 30.60±0.19 MON810 28.5 35.05±0.41 1.18 阳性Positive 27.12±0.16 GTS40-3-2 40.0 36.23±0.40 1.11 阳性Positive 30.43±0.44 G6H1 77.5 33.70±0.38 1.12 阳性Positive 27.49±0.52 TC1507 24.5 35.06±0.88 2.52 阳性Positive 30.34±0.49 59122 36.0 36.11±0.42 1.18 阳性Positive 30.25±0.15 MIR604 27.5 35.82±0.77 2.16 阳性Positive 30.31±0.13 GBH119 67.0 31.56±0.15 0.48 阳性Positive 26.98±0.23