超低温11年保存期对转基因作物基体标准样品核酸检测的影响

王渭霞, 赖凤香, 胡海燕, 何佳春, 魏琪, 万品俊, 傅强

Effect of 11-year storage of GMO reference material at ultra-low temperature on nucleic acid detection of standard matrix sample of transgenic crop

WANG Wei-Xia, LAI Feng-Xiang, HU Hai-Yan, HE Jia-Chun, WEI Qi, WAN Pin-Jun, FU Qiang

表3 单重微滴式数字PCR对G6H1和GBH119标准物质的定量结果

Table 3 Quantitative results of G6H1 and GBH119 with simplex droplet dPCR

样品含量 Sample content (%)	靶标 Target	拷贝数Copies (20 μL^-1)				平均值 Average	转基因含量 GM content (%)	相对标准差RSD (%)	偏差 Bias (%)
样品含量 Sample content (%)	靶标 Target	重复1 Repeat 1	重复2 Repeat 2	重复3 Repeat 3	重复4 Repeat 4	平均值 Average	转基因含量 GM content (%)	相对标准差RSD (%)	偏差 Bias (%)
Kefeng 6 5.0	SPS	175,80.0	180,00.0	183,20.0	191,00.0	182,50.0
Kefeng 6 5.0	Kefeng 6	105,8.0	103,2.0	102,7.0	114,2.0	106,4.8	5.83	5.49	16.60
GBH119 1.0	Adhc	109,000.0	114,000.0	119,400.0	113,800.0	114,050.0
GBH119 1.0	GHB119	952.0	946.0	984.0	916.0	949.5	0.83	3.47	-17.00