超低温11年保存期对转基因作物基体标准样品核酸检测的影响

王渭霞, 赖凤香, 胡海燕, 何佳春, 魏琪, 万品俊, 傅强

Effect of 11-year storage of GMO reference material at ultra-low temperature on nucleic acid detection of standard matrix sample of transgenic crop

WANG Wei-Xia, LAI Feng-Xiang, HU Hai-Yan, HE Jia-Chun, WEI Qi, WAN Pin-Jun, FU Qiang

表4 双重微滴式数字PCR对不同含量的转基因玉米和大豆的定量结果

Table 4 Quantitative results of MON810 and GTS40-3-2 with duplex droplet dPCR

样品含量 Sample content	靶标 Target	拷贝数Copies (20 μL^-1)				转基因含量GM content (%)				平均值 Average (%)	相对标准差 RSD (%)	偏差 Bias (%)
样品含量 Sample content	靶标 Target	重复1 Repeat 1	重复2 Repeat 2	重复3 Repeat 3	重复4 Repeat 4	重复1 Repeat 1	重复2 Repeat 2	重复3 Repeat 3	重复4 Repeat 4	平均值 Average (%)	相对标准差 RSD (%)	偏差 Bias (%)
GTS40-3-2 0.1%	GTS	5.5	4.8	7.4	7.8	0.09	0.08	0.12	0.13	0.11	23.80	10.00
GTS40-3-2 0.1%	Lectin	626,0.0	602,0.0	602,0.0	600,0.0
GTS40-3-2 0.5%	GTS	58.0	64.0	76.0	46.0	0.44	0.49	0.60	0.35	0.47	22.57	6.00
GTS40-3-2 0.5%	Lectin	131,40.0	129,80.0	126,00.0	132,20.0
GTS40-3-2 1.0%	GTS	110.0	106.0	108.0	108.0	1.12	1.10	1.13	1.13	1.12	1.42	12.00
GTS40-3-2 1.0%	Lectin	986,0.0	966,0.0	952,0.0	960,0.0
MON810 0.1%	MON810	6.8	6.8	8.8	10.0	0.08	0.08	0.11	0.13	0.10	21.73	0.00
MON810 0.1%	Adh-1	808,0.0	836,0.0	800,0.0	802,0.0
MON810 0.5%	MON810	55.0	54.0	62.0	56.0	0.51	0.49	0.57	0.52	0.52	6.14	4.00
MON810 0.5%	Adh-1	108,40.0	109,80.0	109,60.0	108,60.0
MON810 1.0%	MON810	196.0	202.0	124.0	166.0	1.21	1.24	0.78	1.04	1.07	19.75	7.00
MON810 1.0%	Adh-1	161,80.0	162,80.0	158,80.0	159,40.0