超低温11年保存期对转基因作物基体标准样品核酸检测的影响

王渭霞, 赖凤香, 胡海燕, 何佳春, 魏琪, 万品俊, 傅强

Effect of 11-year storage of GMO reference material at ultra-low temperature on nucleic acid detection of standard matrix sample of transgenic crop

WANG Wei-Xia, LAI Feng-Xiang, HU Hai-Yan, HE Jia-Chun, WEI Qi, WAN Pin-Jun, FU Qiang

图2 转基因含量0.1%的标准物质转化体特异性普通PCR扩增电泳图 测试样品为低温保存9年的转基因含量0.1%的基体标准物质; M: 100 bp DNA ladder; 1~6: 转基因含量0.1%的标准物质; 7~9: 转基因含量1.0%的阳性对照; 10~12: 非转基因混合物; 13~15: 样品DNA提取空白对照; 16~18: PCR空白水对照。

Fig. 2 Electrophoretic image of GMO reference material with content 0.1% detected by event-specific qualitative PCR The events with 0.1% content were matrix reference materials stored nine years at low temperature; M: 100 bp DNA ladder; 1-6: GMO reference material with 0.1% content; 7-9: positive control with content 1.0% (w/w); 10-12: non-transgenic negative control; 13-15: blank control; 16-18: PCR blank H2O control.