超低温11年保存期对转基因作物基体标准样品核酸检测的影响

王渭霞, 赖凤香, 胡海燕, 何佳春, 魏琪, 万品俊, 傅强

Effect of 11-year storage of GMO reference material at ultra-low temperature on nucleic acid detection of standard matrix sample of transgenic crop

WANG Wei-Xia, LAI Feng-Xiang, HU Hai-Yan, HE Jia-Chun, WEI Qi, WAN Pin-Jun, FU Qiang

图5 存期11年的样品S9和S10中转基因成分的定性PCR检测 M: 100 bp DNA ladder; 1~3: 样品S9; 4~6: 样品S10; 7~9: 转基因含量1.0%的阳性对照; 10~12: 非转基因阴性对照; 13~15: 样品DNA提取空白对照; 16~18: PCR空白水对照; S9和S10为2009年留存的能力验证样品, 存期11年。

Fig. 5 Qualitative PCR detection of transgenic components in S9 and S10 samples stored for 11 year M: 100 bp DNA ladder; 1-3: sample S9; 4-6: sample S10; 7-9: positive control with GM content 1.0% (w/w); 10-12: non-transgenic negative control; 13-15: extract blank control; 16-18: PCR blank H2O control; S9 and S10 are the capability verification samples retained in 2009 with a shelf life of 11 years.