小麦谷氨酰胺合成酶基因可变剪接分析

韦一昊, 于美琴, 张晓娇, 王露露, 张志勇, 马新明, 李会强, 王小纯

Alternative splicing analysis of wheat glutamine synthase genes

WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun

表3 TaGS转录本在IWGSC RefSeq v2.0和第3代测序中的差异

Table 3 Differences of TaGS transcripts in IWGSC RefSeq v2.0 and the third-generation sequencing

TaGS同工酶 TaGS isoforms 基因编号 Gene ID IWGSC RefSeq2.0 第3代测序分析 Third-generation sequencing analysis 转录本编号 Transcript ID 蛋白质 Protein (aa) 分子量 MW (kD) 转录本编号 Transcript ID 蛋白质 Protein (aa) 分子量 MW (kD) CPM* TaGS1;1 TraesCS6A02G298100 TraesCS6A02G298100.1 349 38.4 TraesCS6A02G298100.1 359 39.7 6.4 TraesCS6A02G298100.2 356 39.2 TraesCS6A02G298100.2 356 39.2 103.5 TraesCS6B02G327500 TraesCS6B02G327500.1 356 39.2 TraesCS6B02G327500.1 356 39.2 154.9 TraesCS6B02G327500.2 325 35.8 0 TraesCS6B02G327500.3 322 35.6 TraesCS6B02G327500.3 322 35.6 7.6 ONT.45646.3 223 24.5 3.8 TraesCS6D02G383600LC TraesCS6D02G383600LC.1 356 39.2 TraesCS6D02G383600LC.1 356 39.2 16 TraesCS6D02G383600LC.2 325 35.8 0 TaGS1;2 TraesCS4A02G063800 TraesCS4A02G063800.1 354 38.7 TraesCS4A02G063800.1 354 38.7 114.6 TraesCS4B02G240900 TraesCS4B02G240900.1 354 38.7 TraesCS4B02G240900.1 354 38.7 134.2 TraesCS4D02G240700 TraesCS4D02G240700.1 354 38.7 TraesCS4D02G240700.1 354 38.7 170 TaGS1;3 TraesCS4A02G266900 TraesCS4A02G266900.1 362 39.6 TraesCS4A02G266900.1 362 39.6 8.8 TraesCS4B02G047400 TraesCS4B02G047400.1 362 39.5 TraesCS4B02G047400.1 362 39.5 3.3 TraesCS4D02G047400 TraesCS4D02G047400.1 362 39.5 TraesCS4D02G047400.1 362 39.5 13 TaGS2 TraesCS2A02G500400 TraesCS2A02G500400.1 427 46.7 TraesCS2A02G500400.1 427 46.7 9.7 TraesCS2A02G500400.2 423 46.09 0 TraesCS2B02G528300 TraesCS2B02G528300.1 423 46.08 0 TraesCS2B02G528300.2 369 40.63 TraesCS2B02G528300.2 427 46.7 53 TraesCS2D02G500600 TraesCS2D02G500600.1 427 46.7 TraesCS2D02G500600.1 427 46.7 27.3