| Ta4CL1 F1 | CCGCGGGGAGCAGATCATGAAAGGTTAC | 3′RACE第1轮PCR 1st run PCR of 3′RACE |
| Ta4CL1 F2 | ACATCAAGAAATTCGTCGCAAAGGAGGTT | 3′RACE第2轮PCR 2nd run PCR of 3′RACE |
| Ta4CL1 R1/R2 | AAGCAGTGGTATCAACGCAGAGTAC(T)30N-1N (N=A, C, G or T; N-1=A, G or C) | 3′RACE |
| Ta4CL1 F3/F4 | (T25)N-1N (N=A, C, G or T; N-1=A, G or C) | 5′RACE |
| Ta4CL1 R3 | AACCTCCTTTGCGACGAATTTCTTGATGT | 5′RACE第1轮PCR 1st run PCR of 5′RACE |
| Ta4CL1 R4 | F: TGTCTCCGGTGTGCAGCCATCCAT | 5′RACE第2轮PCR 2nd run PCR of 5′RACE |
| Ta4CL1 F5/R5 | F: CGCACGCACGCACACGCACAA
R: ATCAACATTACACAAGCAGGAAGAACCA | 全长cDNA克隆
Isolation of full-length cDNA |
| Ta4CL1 F6/R6 | F: TCTAGAATGGGGTCTGTGCCGGAG (Xba I)
R: ggTACCCTAGCTTGGGATGCCGGC (Kpn I) | Ta4CL1过表达载体构建
Construction of Ta4CL1-overexpression vector |
| Ta4CL1pro F1/R1 | F:CCAGTGCCCTCCATCTCT
R:CGCCGCCGCAACCGACTC | 克隆Ta4CL1启动子
Cloning of Ta4CL1 promoter |
| Ta4CL1pro F2/R3 | F: AAGCTTCCAGTGCCCTCCATCTCT (Hind III)
R: GAATTCTTCAACGATCGTGGTAGA (EcoR I) | Ta4CL1pro:Gus载体构建
Construction of Ta4CL1 pro:Gus vector |
| GUS | F: GGGCAGGCCAGCGTATCG
R: GTCCCGCTAGTGCCTTGTC | 鉴定Ta4CL1pro:Gus载体
Identification of Ta4CL1pro:Gus vector |
Actin2
(At3g18780) | F: GCTGAGAGATTCAGATGCCC
R: CTCGGCCTTGGAGATCCACA | RT-PCR |
Actin2
(At3g18780) | F: TCGCTGACCGTATGAGCAAAG
R: TGTGAACGATTCCTGGACCTG | qRT-PCR |
AtC3H
(At2g40890) | F: GTTGGACTTGACCGGATCTT
R: ATTAGAGGCGTTGGAGGATG | qRT-PCR |
AtCOMT1-1
(At5g54160) | F: TTCCATTGCTGCTCTTTGTC
R: CATGGTGATTGTGGAATGGT | qRT-PCR |
AtCCoAOMT1
(At4g34050) | F: CTCAGGGAAGTGACAGCAAA
R: GTGGCGAGAAGAGAGTAGCC | qRT-PCR |
AtCAD5
(At4g34230) | F: TTGGCTGATTCGTTGGATTA
R: ATCACTTTCCTCCCAAGCAT | qRT-PCR |
AtF5H
(At4g36220) | F: CTTCAACGTAGCGGATTTCA
R: AGATCATTACGGGCCTTCAC | qRT-PCR |
AtHCT
(At5g48930) | F: GCCTGCACCAAGTATGAAGA
R: GACAGTGTTCCCATCCTCCT | qRT-PCR |
AtCCR1
(At1g15950) | F: GTGCAAAGCAGATCTTCAGG
R: GCCGCAGCATTAATTACAAA | qRT-PCR |
| Ta4CL1 F7/R7 | F: TCGTCGCAAAGGAGGTTGTT
R: GCGGCCGAGTCTGGCTCT | qRT-PCR |
TaEF 1a
(M90077) | F: CAGATTGGCAACGGCTACG
R: CGGACAGCAAAACGACCAAG | qRT-PCR |