小麦Ta4CL1基因的克隆及其在促进转基因拟南芥生长和木质素沉积中的功能

孟颖, 邢蕾蕾, 曹晓红, 郭光艳, 柴建芳, 秘彩莉

Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants

MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li

图1 利用RACE技术克隆Ta4CL1全长cDNA 扩增Ta4CL1的5′ RACE (A)和3′ RACE (B)片段。1: 第1轮PCR; 2: 第2轮PCR; C: Ta4CL1的全长cDNA的扩增。1: Ta4CL1的全长cDNA。M: marker; 箭头所示为目的条带。

Fig. 1 Cloning of full-length cDNA of Ta4CL1 by RACE technique Amplifying the fragments of 5′ RACE (A) and 3′ RACE (B) of Ta4CL1. 1: the 1st run PCR; 2: the 2nd run PCR; C: amplifying the full-length cDNA of Ta4CL. 1: the full-length cDNA of Ta4CL. M: marker; the arrows point to the target bands.