小麦Ta4CL1基因的克隆及其在促进转基因拟南芥生长和木质素沉积中的功能

孟颖, 邢蕾蕾, 曹晓红, 郭光艳, 柴建芳, 秘彩莉

Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants

MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li

图6 异源表达Ta4CL1的拟南芥株系的表型观察 A: 正常生长2周的拟南芥幼苗; B: 正常生长6周的拟南芥幼苗; C: 各拟南芥株系的株高统计; D: 各拟南芥株系的直径统计; one-way ANOVA分析(字母不同表示差异显著, 含有相同字母表示差异不显著; P < 0.05)。Col-0: 野生型拟南芥; OX-1、-2和-3: 过表达Ta4CL1拟南芥纯合株系; Ta4CL1/at4cl1 L3、L9和L11: 在at4cl背景中过表达Ta4CL1的3个纯合株系; Ta4CL1/at4cl3 L10、L12和L13: 在at4cl3背景中过表达Ta4CL1的3个纯合株系; Ta4CL1/at4cl1 4cl3 L6、L10和L11: 在at4cl1 4cl3背景中过表达Ta4CL1的3个纯合株系。

Fig. 6 Ectopic expression of Ta4CL1 affects plant growth in transgenic Arabidopsis seedlings A: two-week-old Arabidopsis seedlings; B: six-week-old Arabidopsis seedlings; the measurement of plant height (C) and diameter (D) of the inflorescence stem of the eight-week-old Arabidopsis seedlings, One-way ANOVA followed by Duncan’s test, columns with different letters indicate significant differences, dataset share same letter means no significant differences at P < 0.05. Col-0, wild-type Arabidopsis plants; OX-1, OX-2, and OX-3: Ta4CL1-overexpressing Arabidopsis lines; Ta4CL1/at4cl1 L3, L9, and L11: Arabidopsis lines overexpressing Ta4CL1 in the at4cl1 background; Ta4CL1/at4cl3 L10, L12, and L13: Arabidopsis lines overexpressing Ta4CL1 in the at4cl3 background; Ta4CL1/at4cl1 4cl3 L6, L10, and L11: Arabidopsis lines overexpressing Ta4CL1 in the at4cl1 4cl3 background.