NtPHYB1与光温信号互作调控烟草种子萌发

李振华, 王显亚, 刘一灵, 赵杰宏

NtPHYB1 interacts with light and temperature signal to regulate seed germination in Nicotiana tabacum L.

LI Zhen-Hua, WANG Xian-Ya, LIU Yi-Ling, ZHAO Jie-Hong

表1 基因型、光照、温度及其互作对烟草种子萌发的影响

Table 1 Effects of genotype, light, temperature and their interactions on seed germination in tobacco

基因型 Genotype (G) 光 Light treatment (L) 温度 Temperature (T, ℃) 均值(L×T×G) Mean value (L×T×G) 标准偏差 Standard deviation 样本数 Sample number NtPHYB1过表达 持续黑暗Dark 15 17.78 qrstu NOP 8.36 9 NtPHYB1-OE 20 72.00 abcdefg ABCDE 9.26 9 25 78.00 abcdef ABC 7.91 9 30 26.78 opqr LMNO 13.14 9 35 2.89 u Q 2.62 9 NtPHYB1 RNA干扰 15 31.83 nopq KLMN 4.92 6 NtPHYB1-RNAi 20 65.67 cdefghi BCDEFG 7.50 6 25 75.00 abcdef ABCD 10.12 6 30 46.00 lmn GHIJKL 8.67 6 35 5.00 tu Q 1.41 6 野生型WT 15 38.00 mno IJKLM 6.93 3 20 85.67 ab AB 2.08 3 25 80.67 abc ABC 8.14 3 30 62.00 fghij CDEFGH 3.46 3 35 5.00 tu Q 2.00 3 NtPHYB1过表达 12 h光照/12 h 黑暗 15 16.22 rstu NOP 10.97 9 NtPHYB1-OE 12 h light/12 h dark 20 48.33 klm FGHIJK 15.36 9 基因型 Genotype (G) 光 Light treatment (L) 温度 Temperature (T, ℃) 均值(L×T×G) Mean value (L×T×G) 标准偏差 Standard deviation 样本数 Sample number 25 63.33 efghi CDEFGH 10.55 9 30 74.11 abcdef ABCD 6.41 9 35 73.67 abcdef ABCD 10.32 9 NtPHYB1 RNA干扰 15 18.83 qrst MNOP 5.12 6 NtPHYB1-RNAi 20 55.00 hijkl DEFGHI 7.90 6 25 64.17 defghi CDEFGH 11.96 6 30 78.50 abcde ABC 9.67 6 35 79.33 abcde ABC 4.37 6 野生型WT 15 21.67 pqrs MNOP 4.93 3 20 69.33 cdefgh ABCDE 7.51 3 25 75.00 abcdef ABCD 2.65 3 30 79.67 abcd ABC 4.51 3 35 87.33 aA 3.06 3 NtPHYB1过表达 持续光照Light 15 8.67 stu OP 6.02 9 NtPHYB1- OE 20 38.11 mno IJKLM 18.31 9 25 53.11 jkl EFGHIJ 22.13 9 30 57.22 ghijk DEFGHI 10.93 9 35 65.00 cdefghi BCDEFG 17.68 9 NtPHYB1 RNA干扰 15 20.67 pqrs MNOP 8.98 6 NtPHYB1-RNAi 20 44.83 lmn HIJKL 7.88 6 25 68.33 cdefghi ABCDEF 8.07 6 30 70.33 bcdefgh ABCDE 11.33 6 35 66.17 cdefghi BCDEF 9.79 6 野生型WT 15 11.33 stu OP 3.51 3 20 34.00 mnop JKLMN 5.29 3 25 65.00 cdefghi BCDEFG 2.65 3 30 74.00 abcdef ABCDE 3.00 3 35 70.67 bcdefgh ABCDE 6.43 3