水稻胚胎和胚乳双缺陷突变体eed1的表型与遗传分析

杨谨, 白爱宁, 白雪, 陈娟, 郭林, 刘春明

Phenotypic and genetic analyses of a rice mutant eed1 with defected embryo and endosperm development

YANG Jin, BAI Ai-Ning, BAI Xue, CHEN Juan, GUO Lin, LIU Chun-Ming

表1 实时荧光定量PCR分析所用的引物

Table 1 Primers for qRT-PCR used in this study

基因名 Genes ID 正向引物序列 Forward sequences (5'-3') 反向引物序列 Reverse sequences (5'-3') Ubiquitin CTGTCAACTGCCGCAAGAAG GGCGAGTGACGCTCTAGTTC OsAGPL1 GGAAGACGGATGATCGAGAAAG CACATGAGATGCACCAACGA OsAGPL2 AGTTCGATTCAAGACGGATAGC CGACTTCCACAGGCAGCTTATT OsAGPL3 AAGCCAGCCATGACCATTTG CACACGGTAGATTCACGAGACAA OsAGPS1 GTGCCACTTAAAGGCACCATT CCCACATTTCAGACACGGTTT OsAGPS2a ACTCCAAGAGCTCGCAGACC GCCTGTAGTTGGCACCCAGA OsAGPS2b AACAATCGAAGCGCGAGAAA GCCTGTAGTTGGCACCCAGA OsSS I GGGCCTTCATGGATCAACC CCGCTTCAAGCATCCTCATC OsS IIa GCTTCCGGTTTGTGTGTTCA CTTAATACTCCCTCAACTCCACCAT OsSS IIc GACCGAAATGCCTTTTTCTCG GGGCTTGGAGCCTCTCCTTA OsSS IIIa GCCTGCCCTGGACTACATTG GCAAACATATGTACACGGTTCTGG OsSS IIIb ATTCCGCTCGCAAGAACTGA CAACCGCAGGATAACGGAAA OsSS IVa GGGAGCGGCTCAAACATAAA CCGTGCACTGACTGCAAAAT OsSS IVb ATGCAGGAAGCCGAGATGTT ACGACAATGGGTGCCAAGAT OsGBSS I OsGBSS II AACGTGGCTGCTCCTTGAA AGGCATCGAGGGTGAGGAG TTGGCAATAAGCCACACACA CCATCTGGCCCACATCTCTA OsBE I OsBE IIa TGGCCATGGAAGAGTTGGC GCCAATGCCAGGAAGATGA CAGAAGCAACTGCTCCACC GCGCAACATAGGATGGGTTT OsBE IIb ATGCTAGAGTTTGACCGC AGTGTGATGGATCCTGCC OsISA1 TGCTCAGCTACTCCTCCATCATC AGGACCGCACAACTTCAACATA OsISA2 TAGAGGTCCTCTTGGAGG AATCAGCTTCTGAGTCACCG OsISA3 ACAGCTTGAGACACTGGGTTGAG GCATCAAGAGGACAACCATCTG OsPUL ACCTTTCTTCCATGCTGG CAAAGGTCTGAAAGATGGG OsDPE2 CAAGTACACCACAAGACCAGCAA CGTCCAACAGCGAATCCAAT OsPHOL TTGGCAGGAAGGTTTCGCT CGAAGCCTGAAGTGAACTTGCT OsPHOH CACCAAGACGAAGCTCATCAAG TTCACTCGTTGCTGGGTTCTC GluA1 CATTTGAGCCAATTCGGAGT GGCCTGATTGTTGGAACTGT GluA2 GCAAGAGCAGGAACAAGGAC CCTCATGGTGCAAAAGGTCT GluA3 TGAAAACCAACCCTGACTCC ACTCATCTCCCCTGTTGTGC GluB1 GCCAAAGTCAGAGCCAAAAG GAACCAATGTGCAACACCAG GluB4 GCGACCAGAAGGCTACAAAG TTGCTTGTTGATCGTTGCTC Glutelinλ TGGCGACCATAGCTTTCTCT GGGTTGTGCCATGGATTTAC Globulin1 ATCGAGAACGGCGAGAAGT GGACGGAGATGGTATGGAGA Globulin2 CGACGAGGTGTTCTACGTCA GTGTTGGCGGAGTAGACGAT 11S-Globulin CACCAAACCCGATCTTCAGT CGGAACAGCTTCTCCATCTC 19kD-Globulin GCCAGTAATTGCAGGGGATA AGGTCACCACCAACGTAAGC Prolamin TCTCCAACCAACAATAGCAATG TGCGTAGCTATCTGTGCCCGTC 10kD-Prolamin TGCAGTATTTCCCACCAACA ACATGAACATGGCTGTGGAG 13kD-Prolamin CACAGCGCAGTTTGATGTTT GCTTGCCGCAATGCTATACT 17kD-Prolamin TTTGATGCTTGCACCTATGG GCAGCTGCTCAGTTTTAGCC RA16 AGGTAGTGATCTCGGCGTTG CCGATTCCTGGCTGACATAG RA17 TTCTCGGTATTGCTCCTCGT CTTATTCCTGGCCGACATTG