大豆PIN-Like (PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能

董衍坤, 黄定全, 高震, 陈栩

Identification, expression profile of soybean PIN-Like (PILS) gene family and its function in symbiotic nitrogen fixation in root nodules

DONG Yan-Kun, HUANG Ding-Quan, GAO Zhen, CHEN Xu

图7 GmPILS1e和GmPILS1f参与大豆根瘤的固氮酶活性调控 A~G: 在接种根瘤菌21 d 后, 对结瘤的大豆复合植株进行表型分析。A: Mock、GmPILS1e 1f-RNAi#1和GmPILS1e 1f-RNAi#2毛根根瘤对半横切的拍照结果, 标尺为200 µm。B: 利用荧光定量PCR检测Mock、GmPILS1e 1f-RNAi#1和GmPILS1e 1f-RNAi#2毛根根瘤的表达, 以GmELF1b基因作为内参基因。C, D: 检测相同重量Mock、GmPILS1e 1f-RNAi#1和GmPILS1e 1f-RNAi#2毛根根瘤的固氮酶活性。E: 统计Mock, GmPILS1e 1f-RNAi#1和GmPILS1e 1f-RNAi#2毛根根瘤横切的菌体区域的面积占比。F: 利用荧光定量PCR检测Mock和35S::GmPILS1f 毛根根瘤的表达, 以GmELF1b基因作为内参基因。G: 检测Mock和35S::GmPILS1f 毛根根瘤的固氮酶活性。*、**、***分别表示在0.05、0.01和0.001水平差异显著; ns: 无显著性差异。

Fig. 7 GmPILS1e and GmPILS1f were involved in the regulation of nitrogenase activity in soybean nodules A-G: phenotype analysis was performed on soybean complex plants after inoculation of rhizobia for 21 days. A: mock, GmPILS1e 1f-RNAi#1 and GmPILS1e 1f-RNAi#2 nodules by semi-crosscutting, bar: 200 µm. B: RT-qPCR analysis of GmPILS1e or GmPILS1f in mock, GmPILS1e 1f-RNAi#1 and GmPILS1e 1f-RNAi#2 nodules, with GmELF1b gene as the internal reference gene. C, D: test the nitrogenase activity of mock, GmPILS1e 1f-RNAi#1 and GmPILS1e 1f-RNAi#2 nodules of the same weight. E: statistic mock, GmPILS1e 1f-RNAi#1 and GmPILS1e 1f-RNAi#2. F: RT-qPCR analysis of GmPILS1f in Mock and 35S::GmPILS1f nodules, with GmELF1b gene as the internal reference gene. G: test the nitrogenase activity of mock and 35S::GmPILS1f nodules. *, **, and *** mean significant difference at the 0.05, 0.01, 0.001 probability levels, respectively. ns: no significant difference.