小麦-中间偃麦草2A/6St代换系014-459的分子细胞遗传学鉴定

陶军, 兰秀锦

Molecular cytogenetic identification of wheat-Thinopyrum intermedium 2A/6St substitution strain 014-459

TAO Jun, LAN Xiu-Jin

图2 原位杂交及花粉母细胞减数分裂图 A: 品系014-459FISH图, Oligo-pSc119.2-2(绿), oligo-pTa535-2(红); B: 品系014-459GISH图, 中间偃麦草DNA作探针, 中国春DNA为封阻; C: 品系014-459GISH图, 拟鹅冠草DNA为探针, 中国春DNA为封阻; D: 品系014-459FISH图, St2-80为探针, 中国春DNA为封阻; E: 品系014-459花粉母细胞减数分裂中期GISH图, 探针为中间偃麦草DNA, 封阻为中国春DNA。

Fig. 2 In situ hybridization and pollen mother-cell meiosis in wheat-Thinopyrum intermedium A: FISH map of strain 014-459. Oligo-pSc119.2-2 (green), oligo-pTa535-2 (red); B: GISH map of strain 014-459. DNA of Th. intermedium used as the probe, and DNA of CS was used as the blocker; C: GISH map of strain 014-459. DNA of Pseudoroegneria strigosa was used as the probe and DNA of CS was used as the blocker; D: FISH map of strain 014-459. St2-80 was used as the probe and DNA of CS was used as the blocker; E: GISH map of pollen mother cells of strain 014-459 at the meiotic metaphase. DNA of Th. intermedium was used as the probe and DNA of CS was used as the blocker.