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作物学报

作物学报 ›› 2026, Vol. 52 ›› Issue (5): 1364-1372.doi: 10.3724/SP.J.1006.2026.52045

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻剑叶小维管束数基因SVN7的鉴定与克隆

闫安1,2(), 蒋昆炜1,4(), 王蓉圆1, 田林1, 张璐1,2, 王韵1,*(), 徐建龙2,3,*()   

  1. 1 沈阳农业大学农学院, 辽宁沈阳 110866
    2 作物基因资源与育种全国重点实验室 / 中国农业科学院作物科学研究所, 北京 100081
    3 岭南现代农业科学与技术广东省实验室深圳分中心 / 中国农业科学院农业基因组研究所, 广东深圳 518120
    4 铁岭市农业科学院, 辽宁铁岭 112616
  • 收稿日期:2025-12-31 接受日期:2026-02-27 出版日期:2026-05-12 网络出版日期:2026-03-05
  • 通讯作者: *王韵, E-mail: wangyun1981@syau.edu.cn; 徐建龙, E-mail: xujianlong@caas.cn
  • 作者简介:闫安, E-mail: 15241886336@163.com;
    蒋昆炜, E-mail: jiangkunweii@163.com
    **同等贡献
  • 基金资助:
    国家重点研发计划项目(2023YFF1000400);辽宁省教育厅基本科研项目(LJ212410157119);国家自然科学基金项目(32072035)

Identification and cloning of SVN7 controlling small vascular bundle number in the rice flag leaf

Yan An1,2(), Jiang Kun-Wei1,4(), Wang Rong-Yuan1, Tian Lin1, Zhang Lu1,2, Wang Yun1,*(), Xu Jian-Long2,3,*()   

  1. 1 Agronomy College of Shenyang Agricultural University, Shenyang 110866, Liaoning, China
    2 State Key Laboratory of Crop Gene Resources and Breeding / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3 Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture / Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, Guangdong, China
    4 Tieling Academy of Agricultural Sciences, Tieling 112616, Liaoning, China
  • Received:2025-12-31 Accepted:2026-02-27 Published:2026-05-12 Published online:2026-03-05
  • Contact: *Wang Yun, E-mail: wangyun1981@syau.edu.cn; Xu Jian-Long, E-mail: xujianlong@caas.cn
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    National Key Research and Development Program of China(2023YFF1000400);Basic Research Project of the Department of Education of Liaoning Province(LJ212410157119);National Natural Science Foundation of China(32072035)

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摘要：

叶片维管束在光合产物的运输过程中起着重要作用, 维管束的数量、大小直接影响着叶片光合产物的运输效率。本研究通过对307份水稻品种的剑叶小维管束数性状进行全基因组关联分析(GWAS), 鉴定到一个控制剑叶小维管束数的基因SVN7, 该基因与油菜素内酯信号传导的下游信号分子基因OsBZR1等位。利用CRISPR/Cas9敲除技术获得了SVN7基因的突变体。与野生型中花11相比, 突变体的剑叶小维管束数显著增加, 表明SVN7基因负调控剑叶小维管束数。除此以外, snv7突变体的穗颈大、小维管束数、剑叶宽度和穗粒数均显著增加, 而株高和粒重显著降低, 说明SVN7基因具有一因多效性, 同时调控源、库、流相关性状。挖掘到了SVN7基因的优异单倍型Hap1, 该单倍型具有较多的剑叶小维管束数、较宽的剑叶、较多的穗粒数和较高的单株产量, 可用于分子设计育种培育高产的水稻新品种。

关键词: 水稻, 剑叶小维管束数, 全基因组关联分析, SVN7基因, 产量

Abstract:

Leaf vascular bundles play a crucial role in the transport of photosynthates, and their number, size, and capacity directly affect transport efficiency. Here, we performed a genome-wide association study (GWAS) of small vascular bundle number (SVN) in the flag leaf using 307 rice cultivars and identified SVN7, an allele of OsBZR1. A SVN7 knockout mutant was generated using CRISPR/Cas9. The flag-leaf SVN of svn7 mutants was significantly higher than that of the wild-type Zhonghua 11 (ZH11), indicating that SVN7 negatively regulates small vascular bundle number. Compared with ZH11, svn7 mutants also showed significantly increased numbers of large and small vascular bundles in the panicle neck, increased flag-leaf width, and increased grain number per panicle, whereas plant height and grain weight were significantly reduced. These results indicate that SVN7 has pleiotropic effects and coordinately regulates source-, sink-, and flow-related traits. Among the 307 accessions, the superior haplotype Hap1 was associated with higher flag-leaf SVN, wider flag leaves, more grains per panicle, and higher yield per plant. This haplotype may be useful for molecular design breeding to develop new high-yield rice varieties.

Key words: rice, small vascular bundle in flag leaf, genome-wide association analysis, SVN7 gene, yield

图1

通过全基因组关联分析鉴定位于第7染色体剑叶小维管束数的候选基因 A: 剑叶小维管束数性状的箱线图; B: 剑叶小维管束数与叶型性状的相关性分析; C: 307份种质资源剑叶小维管束数性状全基因组关联分析的曼哈顿图; D: 7号染色体峰值周围的局部曼哈顿图(上)和连锁不平衡块图(下), 蓝色虚线间表示qSVN7的区间范围; E: LOC_Os07g39220的基因结构; F: LOC_Os07g39220基因单倍型剑叶小维管束数性状的小提琴图。SVN: 小维管束数; FLL: 剑叶长度; FLW: 剑叶宽度; FLA: 剑叶面积; xian: 籼稻; geng: 粳稻。星号表示t检验差异显著。箱线图上的不同字母表示Duncan检验差异显著(P < 0.05)。"

图2

SVN7基因的CRISPR/Cas9敲除突变体的维管束数相关性状分析 A: SVN7的基因编辑靶点和突变体序列; B: 中花11和svn7突变体的株型, 标尺= 10 cm; C: 中花11和svn7突变体的剑叶横截面, 标尺= 200 μm; D: 中花11和svn7突变体的剑叶维管束数; E: 中花11和svn7突变体的穗颈横截面, 标尺= 50 μm; F: 中花11和svn7突变体的穗颈维管束数。SVN: 小维管束数; LVN: 大维管束数; ZH11: 中花11。星号表示t检验差异显著; ns表示差异不显著。"

图3

中花11和svn7突变体的产量及产量相关性状的表型 A: 中花11和svn7突变体的叶型, 标尺= 5 cm; B: 中花11和svn7突变体的剑叶相关性状的表型; C: 中花11和svn7突变体的穗型, 标尺= 1 cm; D: 中花11和svn7突变体的产量及产量相关性状的表型。星号表示t检验差异显著; ns表示差异不显著。"

图4

SVN7的单倍型分析 A: SVN7的单倍型; B: 携带Hap1、Hap2和Hap3的种质资源(个体数> 20)产量及产量相关性状比较。小提琴上的不同字母表示Duncan检验差异显著。"

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