外源褪黑素通过协调光反应与暗反应增强玉米苗期的耐热性

doi:10.3724/SP.J.1006.2026.53044

作物学报 ›› 2026, Vol. 52 ›› Issue (3): 839-856.doi: 10.3724/SP.J.1006.2026.53044

所属专题：玉米：耕作栽培·生理生化

外源褪黑素通过协调光反应与暗反应增强玉米苗期的耐热性

李新浩¹(), 邢梦柯¹, 周梓惠¹, 李思烨², 任昊², 王洪章², 赖华江²^,^*()

¹山东农业大学未来技术学院, 山东泰安 271018
²山东农业大学农学院, 山东泰安 271018

收稿日期:2025-07-01 接受日期:2025-11-18 出版日期:2026-03-12 网络出版日期:2025-12-01
通讯作者: *赖华江, E-mail: lhj955297@126.com
作者简介:E-mail: 2369034411@qq.com
基金资助:
山东省重点研发计划项目(2022CXPT014-02);优良品种产效协同提升关键技术研究课题项目

Exogenous melatonin enhances heat tolerance of maize at the seedling stage by coordinating light and dark reactions

Li Xin-Hao¹(), Xing Meng-Ke¹, Zhou Zi-Hui¹, Li Si-Ye², Ren Hao², Wang Hong-Zhang², Lai Hua-Jiang²^,^*()

¹College of Future Technology, Shandong Agricultural University, Tai’an 271018, Shandong, China
²College of Agronomy, Shandong Agricultural University, Tai’an 271018, Shandong, China

Received:2025-07-01 Accepted:2025-11-18 Published:2026-03-12 Published online:2025-12-01
Contact: *赖华江, E-mail: lhj955297@126.com
Supported by:
Key Research and Development Program of Shandong Province(2022CXPT014-02);Project of Key Technologies for Synergistic Improvement of Yield and Efficiency of Elite Varieties

摘要/Abstract

摘要：

近年来, 频发的极端高温天气严重制约了我国玉米生产。外源褪黑素(Met)通过提高抗氧化能力来增强作物的抗逆性, 但其对热胁迫下玉米叶片碳同化能力及原初光化学效率的影响机制鲜有报道。本研究以热敏感型玉米品种先玉335 (XY)和耐热型玉米品种登海605 (DH)为材料, 于玉米6叶期进行热胁迫(38℃/28℃)处理, 探究外源Met对光合响应特征和荧光动力学曲线的影响。结果显示, 与清水处理相比, 外源Met促进了热胁迫下玉米叶片中可溶性糖和脯氨酸的积累, 提高了植株含水量。Met还提高了超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性, 减少了氧化胁迫和丙二醛(MDA)含量。这些改善效应降低了K相(300 μs)和L相(200 μs)的相对荧光, 缓解了热胁迫对放氧复合体的损伤和电子传递阻碍, 并提高了单位面积PSII反应中心数量。XY和DH的最大光化学效率(?_Po)在Met处理下分别提高29.1%和17.2%。此外, 与清水处理相比, 外源Met改善了热胁迫下玉米叶片核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)和磷酸烯醇式丙酮酸羧化酶(PEPC)的活性, 尤其是在敏感品种XY中。光合响应参数显示, Met处理下, XY和DH的最大光合效率(P_nmax)分别提高了38.6%和30.8%, 光合能力(P_c)分别提高了30.2%和19.2%, 并降低了光呼吸速率(R_p), 提高了光饱和点(I_sat)、CO₂饱和点(C_isat)和最大羧化效率(V_cmax)。研究表明, 外源Met增强了叶片的渗透调节能力和抗氧化能力, 协同改善了光反应和暗反应, 缓解了热胁迫对玉米苗期净光合速率(P_n)的影响和对植株生长的损伤。

关键词: 玉米, 高温胁迫, 褪黑素, 叶绿素a荧光动力学, 光响应曲线, CO₂响应曲线

Abstract:

In recent years, frequent extreme high-temperature events have severely limited maize production in China. Exogenous melatonin (Met) has been shown to enhance crop stress tolerance by improving antioxidant capacity; however, its role in regulating carbon assimilation and primary photochemical efficiency in maize leaves under heat stress remains poorly understood. In this study, the heat-sensitive maize cultivar Xianyu 335 (XY) and heat-tolerant cultivar Denghai 605 (DH) were used to investigate the effects of exogenous Met on photosynthetic responses and chlorophyll fluorescence kinetic curves under heat stress conditions (38℃/28℃) at the six-leaf stage. Compared with water treatment, exogenous Met significantly promoted the accumulation of soluble sugars and proline in maize leaves under heat stress, enhanced plant water content, and increased the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), thereby reducing oxidative damage and lowering malondialdehyde (MDA) levels. These improvements alleviated the rise in relative fluorescence at the K phase (300 μs) and L phase (200 μs), mitigated heat-induced damage to the oxygen-evolving complex, relieved electron transport blockages, and increased the number of PSII reaction centers per unit area. Under Met treatment, the maximum photochemical efficiency (?_Po) increased by 29.1% in XY and 17.2% in DH. Furthermore, Met enhanced the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC), especially in the heat-sensitive XY. Photosynthetic response analysis showed that under Met treatment, the maximum net photosynthetic rate (P_nmax) increased by 38.6% in XY and 30.8% in DH, while photosynthetic capacity (P_c) increased by 30.2% and 19.2%, respectively. In addition, Met reduced the photorespiration rate (R_p) and increased the light saturation point (I_sat), CO₂ saturation point (C_isat), and maximum carboxylation efficiency (V_cmax). Overall, these findings indicate that exogenous Met enhances osmotic adjustment and antioxidant defense in maize leaves, synergistically improves both light and dark reactions of photosynthesis, and effectively mitigates heat stress-induced damage to net photosynthesis (P_n) and early-stage plant growth.

Key words: maize, heat stress, melatonin, chlorophyll a fluorescence kinetics, light response curve, CO₂ response curve

李新浩, 邢梦柯, 周梓惠, 李思烨, 任昊, 王洪章, 赖华江. 外源褪黑素通过协调光反应与暗反应增强玉米苗期的耐热性[J]. 作物学报, 2026, 52(3): 839-856.

Li Xin-Hao, Xing Meng-Ke, Zhou Zi-Hui, Li Si-Ye, Ren Hao, Wang Hong-Zhang, Lai Hua-Jiang. Exogenous melatonin enhances heat tolerance of maize at the seedling stage by coordinating light and dark reactions[J]. Acta Agronomica Sinica, 2026, 52(3): 839-856.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2026.53044

https://zwxb.chinacrops.org/CN/Y2026/V52/I3/839

图/表 14

表1

荧光瞬态OJIP曲线的JIP-test相关术语的说明和计算公式"

参数和计算公式 Parameter and formulae	参数说明 Parameter illustration
V_t = (F_t-F_o)/(F_m-F_o)	O相到P相之间的相对可变荧光。F_t为脉冲光诱导t时刻的荧光强度; F_o为最小荧光(所有PSII反应中心(RC)开放时, t = 0时刻); F_m为最大荧光(所有PSII反应中心关闭时)。V_j和V_i分别表示在J相(2 ms)和I相(30 ms)的相对可变荧光 Relative variable fluorescence between phase O and phase P. F_t, fluorescence at time t after onset of actinic illumination; F_o, minimal fluorescence, when all PSII reaction centers (RC) are open (at t = 0); F_m, maximal fluorescence, when all PSII RCs are closed. V_j and V_i represent the relatively variable fluorescence in the J phase (2 ms) and I phase (30 ms), respectively
W_k = (F_t-F_o)/(F_j-F_o)	O相到J相之间的相对可变荧光。F_j为2 ms时的荧光强度 Relative variable fluorescence between phase O and phase J. F_j, fluorescence at 2 ms after onset of actinic illumination
L_band= (F_t-F_o)/(F_k-F_o)	O相和K相之间的相对可变荧光。F_k为300 μs时的荧光强度 Relative variable fluorescence between phase O and phase K. F_k, fluorescence at 300 μs after onset of actinic illumination
PI_abs= (ϕ_Po/(1-ϕ_Po)) (ψ_o/(1-ψ_o)) (1-ABS/RC)	基于吸收光能为基础的性能指数 Performance index on absorption basis
ϕ_Po = TR_o/ABS = [1- (F_o/F_m)]	原初光化学的最大量子产额(在t = 0时) Maximum quantum yield of primary photochemistry (at t = 0)
Ψ_o = ET_o/TR_o = 1-V_j	将电子传递至Q_A^-之后的电子传递链的概率(在t = 0时) Probability that a trapped exciton moves an electron into the electron transport chain beyond Q_A^- (at t = 0)
ϕ_Eo = ET_o/ABS = (1-F_o/F_m) ψ_o	用于电子传递的量子产额(在t = 0时) Quantum yield for electron transport (at t = 0)
ϕ_Ro = RE_o/ABS = TR_o/ABS (1-V_i)	光系统I (PSI)末端电子受体还原的量子产额 The quantum yield of reduction of end electron acceptors of PSI
ABS/RC = M_o(1/V_j) (1/ϕ_Po)	单位反应中心吸收的光能。M_o, OJIP荧光诱导曲线的初始斜率 Absorption flux per RC. M_o, the initial slope of the OJIP fluorescence induction curve
TR_o/RC = M_o(1/V_j)	单位反应中心捕获的用于还原Q_A的能量(在t = 0时) Energy trapped per RC for Q_A reduction (at t = 0)
ET_o/RC = M_o(1/V_j) ψ_o	单位反应中心捕获的用于电子传递的能量(在t = 0时) Energy trapped per RC for electron transport (at t = 0)
DI_o/RC = ABS/RC-TR_o/RC	单位反应中心耗散掉的能量(在t = 0时) Energy dissipated per RC (at t = 0)
RE_o/RC = ϕ_Ro(ET_o/RC)	RC为基础的电子传递到PSI受体侧末端电子受体的能量(在t = 0时) RC-based energy for electron transport to the terminal electron acceptors at the PSI acceptor side (at t = 0)
RC/CS_o = ϕ_Po(V_j/M_o) (ABS/CS_o)	单位面积有活性反应中心的数量 Density of active reaction centers

表1

图1

图2

图3

图4

表2

表3

图5

图6

图7

图8

图9

图10

图11

参考文献 53

[1]	Ai Y F, Jane J L. Macronutrients in corn and human nutrition. Compr Rev Food Sci Food Saf, 2016, 15: 581-598. doi: 10.1111/crf3.2016.15.issue-3
[2]	国家统计局.国家统计局关于2024年粮食产量数据的公告(2024-12-13).https://www.stats.gov.cn/sj/zxfb/202412/t20241213_1957744.html
	National Bureau of Statistics. Announcement of the National Bureau of Statistics on the Grain Output Data for 2024 (2024-12-13).https://www.stats.gov.cn/sj/zxfb/202412/t20241213_1957744.html (in Chinese).
[3]	刘水苗, 关小康, 赵志恒, 等. 冬麦播前耕作方式对麦玉轮作体系中玉米季土壤水分、籽粒灌浆特征及产量的影响. 中国农业科学, 2024, 57: 3568-3585. doi: 10.3864/j.issn.0578-1752.2024.18.005
	Liu S M, Guan X K, Zhao Z H, et al. Residual effects of tillage regime on soil moisture dynamics, grain filling characteristics and yield of summer maize in wheat-maize double cropping system. Sci Agric Sin, 2024, 57: 3568-3585 (in Chinese with English abstract). doi: 10.3864/j.issn.0578-1752.2024.18.005
[4]	任寒, 刘鹏, 董树亭, 等. 高温胁迫影响玉米生长发育的生理机制研究进展. 玉米科学, 2019, 27(5): 109-115.
	Ren H, Liu P, Dong S T, et al. Research advancements of effect of high temperature stress on growth and development of maize. J Maize Sci, 2019, 27(5): 109-115 (in Chinese with English abstract).
[5]	张川, 刘栋, 王洪章, 等. 不同时期高温胁迫对夏玉米物质生产性能及籽粒产量的影响. 中国农业科学, 2022, 55: 3710-3722. doi: 10.3864/j.issn.0578-1752.2022.19.003
	Zhang C, Liu D, Wang H Z, et al. Effects of high temperature stress in different periods on dry matter production and grain yield of summer maize. Sci Agric Sin, 2022, 55: 3710-3722 (in Chinese with English abstract). doi: 10.3864/j.issn.0578-1752.2022.19.003
[6]	陈志英, 胡健, 韩梦琳, 等. 花后高温对玉米叶片光合特性、差异表达基因及关键通路的影响. 玉米科学, 2025, 33(4): 56-64.
	Chen Z Y, Hu J, Han M L, et al. Effects of post-anthesis high temperature on photosynthetic characteristics, differentially expressed genes and key pathways in maize leaves. J Maize Sci, 2025, 33(4): 56-64 (in Chinese with English abstract).
[7]	Lai H J, Zhang C, Ren H, et al. The continuous damage to nitrogen metabolism caused by heat stress limited the young ear growth and yield of maize (Zea mays L.). Eur J Agron, 2025, 168: 127627. doi: 10.1016/j.eja.2025.127627
[8]	吕梦薇, 胡笑涛, 范晓懂, 等. 拔节期高温干旱复合胁迫对夏玉米生长发育的影响. 干旱地区农业研究, 2022, 40(6): 82-89.
	Lyu M W, Hu X T, Fan X D, et al. Effects of combined stress of high temperature and drought at jointing stage on summer maize growth and development. Agric Res Arid Areas, 2022, 40(6): 82-89 (in Chinese with English abstract).
[9]	Sharkey T D. Effects of moderate heat stress on photosynthesis: importance of thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene. Plant Cell Environ, 2005, 28: 269-277. doi: 10.1111/pce.2005.28.issue-3
[10]	高冠龙, 冯起, 张小由, 等. 植物叶片光合作用的气孔与非气孔限制研究综述. 干旱区研究, 2018, 35: 929-937. doi: 10.13866/j.azr.2018.04.22
	Gao G L, Feng Q, Zhang X Y, et al. An overview of stomatal and non-stomatal limitations to photosynthesis of plants. Arid Zone Res, 2018, 35: 929-937 (in Chinese with English abstract). doi: 10.13866/j.azr.2018.04.22
[11]	Li Y T, Xu W W, Ren B Z, et al. High temperature reduces photosynthesis in maize leaves by damaging chloroplast ultrastructure and photosystem II. J Agron Crop Sci, 2020, 206: 548-564. doi: 10.1111/jac.v206.5
[12]	Lai H J, Li X D, Chen Y L, et al. Mitigating heat-induced yield loss in peanut: insights into 24-epibrassinolide-mediated improvement in antioxidant capacity, photosynthesis, and kernel weight. Field Crops Res, 2024, 316: 109521. doi: 10.1016/j.fcr.2024.109521
[13]	陈岩, 岳丽杰, 刘永红, 等. 营养生长期持续高温处理对玉米叶片转录组及生化指标的影响. 玉米科学, 2022, 30(4): 48-55.
	Chen Y, Yue L J, Liu Y H, et al. Effects of continuous high temperature treatment during vegetative stages on maize leaf transcriptome and biochemical indicators. J Maize Sci, 2022, 30(4): 48-55 (in Chinese with English abstract).
[14]	牛丽, 刘源, 于康珂, 等. 玉米杂交种苗期耐热性评价. 玉米科学, 2015, 23(1): 107-114.
	Niu L, Liu Y, Yu K K, et al. Evaluation of heat-tolerance of maize hybrids at seedling stage. J Maize Sci, 2015, 23(1): 107-114 (in Chinese with English abstract).
[15]	王蕊, 杨小龙, 须晖, 等. 高等植物褪黑素的合成和代谢研究进展. 植物生理学报, 2016, 52: 615-627.
	Wang R, Yang X L, Xu H, et al. Research progress of melatonin biosynthesis and metabolism in higher plants. Plant Physiol J, 2016, 52: 615-627 (in Chinese with English abstract).
[16]	Pan Y, Xu X S, Li L, et al. Melatonin-mediated development and abiotic stress tolerance in plants. Front Plant Sci, 2023, 14: 1100827. doi: 10.3389/fpls.2023.1100827
[17]	Colombage R, Singh M B, Bhalla P L. Melatonin and abiotic stress tolerance in crop plants. Int J Mol Sci, 2023, 24: 7447. doi: 10.3390/ijms24087447
[18]	Alam M N, Zhang L H, Yang L, et al. Transcriptomic profiling of tall fescue in response to heat stress and improved thermotolerance by melatonin and 24-epibrassinolide. BMC Genomics, 2018, 19: 224. doi: 10.1186/s12864-018-4588-y pmid: 29587632
[19]	余梦奇, 路梦莉, 张雅婷, 等. 褪黑素对高温胁迫玉米叶片光合特性与抗氧化酶活性的调控. 玉米科学, 2024, 32(1): 90-99.
	Yu M Q, Lu M L, Zhang Y T, et al. Regulation of melatonin on photosynthetic characteristics and antioxidant enzyme activities in maize leaves under high temperature stress condition. J Maize Sci, 2024, 32(1): 90-99 (in Chinese with English abstract).
[20]	Wang H Q, Sun J, Ren H, et al. Inhibiting reactive oxygen species production mitigates endoplasmic reticulum damage in florets of developing maize ears under heat stress. Plant J, 2025, 122: e70243.
[21]	Li Z G, Xu Y, Bai L K, et al. Melatonin enhances thermotolerance of maize seedlings (Zea mays L.) by modulating antioxidant defense, methylglyoxal detoxification, and osmoregulation systems. Protoplasma, 2019, 256: 471-490. doi: 10.1007/s00709-018-1311-4
[22]	Cao L R, Fahim A M, Liang X H, et al. Melatonin enhances heat tolerance via increasing antioxidant enzyme activities and osmotic regulatory substances by upregulating zmeno 1 expression in maize (Zea mays L.). Antioxidants, 2024, 13: 1144. doi: 10.3390/antiox13091144
[23]	Ye Z P, Suggett D J, Robakowski P, et al. A mechanistic model for the photosynthesis-light response based on the photosynthetic electron transport of photosystem II in C₃ and C₄ species. New Phytol, 2013, 199: 110-120. doi: 10.1111/nph.2013.199.issue-1
[24]	叶子飘. 光合作用对光和CO₂响应模型的研究进展. 植物生态学报, 2010, 34: 727-740. doi: 10.3773/j.issn.1005-264x.2010.06.012
	Ye Z P. A review on modeling of responses of photosynthesis to light and CO₂. Chin J Plant Ecol, 2010, 34: 727-740 (in Chinese with English abstract).
[25]	叶子飘, 于强. 光合作用对胞间和大气CO₂响应曲线的比较. 生态学杂志, 2009, 28: 2233-2238.
	Ye Z P, Yu Q. A comparison of response curves of winter wheat photosynthesis to flag leaf intercellular and air CO₂ concentrations. Chin J Ecol, 2009, 28: 2233-2238 (in Chinese with English abstract).
[26]	Strasser R J, Tsimilli-Michael M, Srivastava A. Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou G C, Govindjee G, eds. Chlorophyll a Fluorescence: a Signature of Photosynthesis. Dordrecht: Springer Press, 2004. pp 321-362.
[27]	Giannopolitis C N, Ries S K. Superoxide dismutases: I. occurrence in higher plants. Plant Physiol, 1977, 59: 309-314.
[28]	Rao M V, Hale B A, Ormrod D P. Amelioration of ozone-induced oxidative damage in wheat plants grown under high carbon dioxide (role of antioxidant enzymes). Plant Physiol, 1995, 109: 421-432. pmid: 12228603
[29]	Aebi H. In: Bergmeyer H U, eds. Methods of Enzymatic Analysis. Weinheim: Verlag Chemie Press, 1974. pp 673-684.
[30]	赵世杰, 苍晶. 植物生理学实验指导. 北京: 中国农业出版社, 2015. pp 234-236.
	Zhao S J, Cang J. Experimental Guidance in Plant Physiology. Beijing: China Agriculture Press, 2015. pp 234-236 (in Chinese).
[31]	李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
	Li H S. Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2000 (in Chinese).
[32]	魏爱丽, 张英华, 黄琴, 等. 小麦不同绿色器官光合速率与碳同化酶活性及其基因型差异研究. 作物学报, 2007, 33: 1426-1431.
	Wei A L, Zhang Y H, Huang Q, et al. Dynamic characteristics of photosynthetic rate and carbon assimilation enzyme activities of different green organs in different genotypes of wheat. Acta Agron Sin, 2007, 33: 1426-1431 (in Chinese with English abstract).
[33]	Fahad S, Bajwa A A, Nazir U, et al. Crop production under drought and heat stress: plant responses and management options. Front Plant Sci, 2017, 8: 1147. doi: 10.3389/fpls.2017.01147 pmid: 28706531
[34]	Moore C E, Meacham-Hensold K, Lemonnier P, et al. The effect of increasing temperature on crop photosynthesis: from enzymes to ecosystems. J Exp Bot, 2021, 72: 2822-2844. doi: 10.1093/jxb/erab090 pmid: 33619527
[35]	张玲玲. 5种植物光响应曲线最适拟合模型筛选. 西南林业大学学报, 2017, 37(1): 20-25.
	Zhang L L. Light response curves of 5 plants and screening the optimal model. J Southwest For Univ (Nat Sci), 2017, 37(1): 20-25 (in Chinese with English abstract).
[36]	王欢利, 曹福亮, 刘新亮. 高温胁迫下不同叶色银杏嫁接苗光响应曲线的拟合. 南京林业大学学报(自然科学版), 2015, 39(2): 14-20. doi: 10.3969/j.issn.1000-2006.2015.02.003
	Wang H L, Cao F L, Liu X L. Fitting the light response curves of two Ginkgo variants under heating stress. J Nanjing For Univ (Nat Sci Edn), 2015, 39(2): 14-20 (in Chinese with English abstract).
[37]	蔡福, 米娜, 纪瑞鹏, 等. 关键生育阶段持续干旱对东北春玉米光合特性的影响. 生态学杂志, 2017, 36: 1578-1589.
	Cai F, Mi N, Ji R P, et al. Effects of continuous drought stress during key growth phases on photosynthetic characteristics of spring maize in Northeast China. Chin J Ecol, 2017, 36: 1578-1589 (in Chinese with English abstract).
[38]	张曦文, 刘铁东, 程国侦, 等. 不同光处理对玉米叶片光响应曲线和二氧化碳响应曲线的影响. 辽宁农业科学, 2018(1): 13-16.
	Zhang X W, Liu T D, Cheng G Z, et al. Effects of different light treatments on light response curve and carbon dioxide response curve of maize leaves. Liaoning Agric Sci, 2018(1): 13-16 (in Chinese with English abstract).
[39]	姚斌, 徐晨, 王俊鹏, 等. 不同父本血缘玉米杂交种对光、CO₂响应特性. 分子植物育种, 2020, 18: 1297-1306.
	Yao B, Xu C, Wang J P, et al. Light and CO₂ response characteristics of different parental bloodline maize hybrids. Mol Plant Breed, 2020, 18: 1297-1306 (in Chinese with English abstract).
[40]	Betti M, Bauwe H, Busch F A, et al. Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement. J Exp Bot, 2016, 67: 2977-2988. doi: 10.1093/jxb/erw076 pmid: 26951371
[41]	钟孝芬, 李波娣, 李敏姬, 等. 光呼吸研究进展. 热带亚热带植物学报, 2022, 30: 782-790.
	Zhong X F, Li B D, Li M J, et al. Research advances in photorespiration. J Trop Subtrop Bot, 2022, 30: 782-790 (in Chinese with English abstract).
[42]	Kutschera U, Pieruschka R, Berry J A. Leaf development, gas exchange characteristics, and photorespiratory activity in maize seedlings. Photosynthetica, 2010, 48: 617-622. doi: 10.1007/s11099-010-0079-3
[43]	Cavanagh A P, South P F, Bernacchi C J, et al. Alternative pathway to photorespiration protects growth and productivity at elevated temperatures in a model crop. Plant Biotechnol J, 2022, 20: 711-721. doi: 10.1111/pbi.v20.4
[44]	Hasanuzzaman M, Nahar K, Alam M M, et al. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J Mol Sci, 2013, 14: 9643-9684. doi: 10.3390/ijms14059643 pmid: 23644891
[45]	佟莉蓉, 倪顺刚, 任星远, 等. 褪黑素对干旱胁迫下达乌里胡枝子幼苗生长及叶片水分生理的影响. 草地学报, 2021, 29: 1682-1688. doi: 10.11733/j.issn.1007-0435.2021.08.010
	Tong L R, Ni S G, Ren X Y, et al. Effects of melatonin on seedling growth and leaf water physiology of Lespedeza davurica under drought stress. Acta Agrest Sin, 2021, 29: 1682-1688 (in Chinese with English abstract).
[46]	Urban J, Ingwers M W, McGuire M A, et al. Increase in leaf temperature opens stomata and decouples net photosynthesis from stomatal conductance in Pinus taeda and Populus deltoides × nigra. J Exp Bot, 2017, 68: 1757-1767. doi: 10.1093/jxb/erx052
[47]	Liu Y N, Xu Q Z, Li W C, et al. Long-term high light stress induces leaf senescence in wheat (Triticum aestivum L.). Photosynthetica, 2019, 57: 830-840. doi: 10.32615/ps.2019.086
[48]	Ma Y D, Wang B, Zhang R M, et al. Initial simulated acid rain impacts reactive oxygen species metabolism and photosynthetic abilities in Cinnamonum Camphora undergoing high temperature. Ind Crops Prod, 2019, 135: 352-361. doi: 10.1016/j.indcrop.2019.04.050
[49]	Fahad S, Bajwa A A, Nazir U, et al. Crop production under drought and heat stress: plant responses and management options. Front Plant Sci, 2017, 8: 1147. doi: 10.3389/fpls.2017.01147 pmid: 28706531
[50]	Jarvis P. Targeting of nucleus-encoded proteins to chloroplasts in plants. New Phytol, 2008, 179: 257-285. doi: 10.1111/j.1469-8137.2008.02452.x pmid: 19086173
[51]	Kikuchi S, Bédard J, Hirano M, et al. Uncovering the protein translocon at the chloroplast inner envelope membrane. Science, 2013, 339: 571-574. doi: 10.1126/science.1229262 pmid: 23372012
[52]	Zhang Z S, Liu M J, Scheibe R, et al. Contribution of the alternative respiratory pathway to PSII photoprotection in C₃ and C₄ plants. Mol Plant, 2017, 10: 131-142. doi: 10.1016/j.molp.2016.10.004
[53]	Nunes-Nesi A, Araújo W L, Fernie A R. Targeting mitochondrial metabolism and machinery as a means to enhance photosynthesis. Plant Physiol, 2011, 155: 101-107. doi: 10.1104/pp.110.163816 pmid: 20966153

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

年份 Year	品种 Cultivar	处理 Treatment	暗呼吸速率 R_d (μmol m^-2 s^-1)	最大净光合速率P_nmax(μmol m^-2 s^-1)	饱和光强 I_sat(μmol m^-2 s^-1)	光补偿点 I_c (μmol m^-2 s^-1)	表观量子效率 AQY (mmol m^-2 s^-1)
2024	XY	Met	2.77±0.60 a	14.96±0.60 a	1044.03±45.50 a	59.69±5.56 a	0.038±0.005 a
		Water	2.58±0.41 a	11.56±0.64 b	910.61±27.79 b	68.40±12.45 a	0.032±0.006 a
2025	XY	Met	3.36±0.73 a	24.00±0.47 a	1460.49±8.75 b	65.67±12.15 a	0.040±0.003 a
		Water	4.04±0.50 a	17.31±1.52 c	1219.31±107.46 c	68.15±7.49 a	0.050±0.008 a
	DH	Met	3.53±0.42 a	19.85±0.48 b	1917.19±64.26 a	72.36±3.84 a	0.040±0.005 a
		Water	3.66±0.15 a	15.18±0.43 d	1428.02±165.9 bc	73.20±12.65 a	0.040±0.003 a
方差分析 ANOVA		品种 Cultivar (C)	ns	**	**	ns	ns
		处理Treatment (T)	ns	**	**	ns	ns
		C × T	ns	ns	ns	ns	ns

年份 Year	品种 Cultivar	处理 Treatment	光呼吸速率 R_p (μmol m^-2 s^-1)	光合能力 P_c (μmol m^-2 s^-1)	饱和胞间CO₂浓度 C_isat(μmol mol^-l)	CO₂补偿点 Γ (μmol mol^-l)	最大羧化速率 V_cmax(μmol m^-2 s^-1)
2024	XY	Met	14.73±2.15 b	14.86±0.54 a	558.11±37.48 a	32.73±0.48 a	17.63±0.45 a
		Water	35.48±2.31 a	10.71±0.05 b	470.25±12.26 b	35.73±2.06 a	13.30±0.37 b
2025	XY	Met	4.57±1.28 b	27.10±0.54 a	355.88±50.92 b	4.81±0.49 b	47.55±2.63 a
		Water	9.80±0.56 a	22.27±0.45 b	404.69±53.29 b	33.13±5.14 a	35.21±0.83 b
	DH	Met	1.18±0.37 c	19.61±0.86 c	760.57±174.45 a	3.51±0.55 b	26.29±4.17 c
		Water	1.88±0.55 c	16.45±0.45 d	481.59±62.78 b	4.11±0.38 b	21.67±1.55 c
方差分析 ANOVA		品种 Cultivar (C)	**	**	**	**	**
		处理 Treatment (T)	**	**	ns	**	**
		C × T	**	ns	*	**	ns

外源褪黑素通过协调光反应与暗反应增强玉米苗期的耐热性

Exogenous melatonin enhances heat tolerance of maize at the seedling stage by coordinating light and dark reactions

RichHTML

PDF

摘要/Abstract

引用本文

使用本文

图/表 14

参考文献 53

相关文章 15

Metrics

本文评价

推荐阅读 0

关于本刊

在线期刊

作者中心

相关单位

联系我们

[1]	刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[2]	梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[3]	孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[4]	张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[5]	杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[6]	张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[7]	杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[8]	韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[9]	刘昕萌, 任昊, 张继波, 张吉旺, 赵斌, 任佰朝, 刘鹏, 王洪章. 茉莉酸甲酯(MeJA)缓解高温影响玉米雌穗分化的生理机制[J]. 作物学报, 2026, 52(5): 1561-1572.
[10]	蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180.
[11]	田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005.
[12]	杨亚莉, 徐明睿, 马越飞, 海艺蕊, 刘凯栋, 刘万茂, 孙颖. 玉米根尖及整根响应缺铁的转录组比较研究[J]. 作物学报, 2026, 52(4): 1006-1021.
[13]	张超, 郭欢, 李忠玲, 岳淑宁, 赵娜. 基于BSA-seq技术定位玉米籽粒花青素关联基因[J]. 作物学报, 2026, 52(3): 780-789.
[14]	郭向阳, 涂亮, 王栋, 刘鹏飞, 王安贵, 易强, 任洪, 李刚, 祝云芳, 吴迅, 蒋喻林, 田丰, 陈泽辉. 热带Suwan种质在我国玉米种质改良中的创新与利用[J]. 作物学报, 2026, 52(3): 655-664.
[15]	马亮, 马璐, 张舒钰, 章慧敏, 王仁明, 宋旭东, 张振良, 冒宇翔, 陆虎华, 陈国清, 郝德荣, 周广飞. 玉米苞叶数目转录组分析及候选基因鉴定[J]. 作物学报, 2026, 52(3): 790-801.