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作物学报 ›› 2026, Vol. 52 ›› Issue (3): 665-676.doi: 10.3724/SP.J.1006.2026.54111

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

马铃薯UDP-糖基转移酶基因StUGT52的克隆及功能验证

汪玲(), 胡好(), 宋家凤, 程洁蓝, 陈颖, 郑婷婷, 吕钊彦, 朱晓彪, 侯华兰()   

  1. 安徽农业大学园艺学院 / 安徽省园艺作物育种工程中心, 安徽合肥 230036
  • 收稿日期:2025-09-20 接受日期:2025-11-18 出版日期:2026-03-12 网络出版日期:2025-11-25
  • 通讯作者: *侯华兰, E-mail: hhl@ahau.edu.cn
  • 作者简介:汪玲, E-mail: 2658813298@qq.com;
    胡好, E-mail: 2410509089@qq.com

    **同等贡献

  • 基金资助:
    国家自然科学基金项目(32201853);安徽省自然科学基金项目(2108085QC122);安徽省高校自然科学基金项目(KJ2020A0098);安徽省大学生创新创业训练计划项目(S202410364070)

Cloning and functional validation of UDP-glycosyltransferase gene StUGT52 in potato

Wang Ling(), Hu Hao(), Song Jia-Feng, Cheng Jie-Lan, Chen Ying, Zheng Ting-Ting, Lyu Zhao-Yan, Zhu Xiao-Biao, Hou Hua-Lan()   

  1. College of Horticulture, Anhui Agricultural University / Anhui Provincial Engineering Center of Horticultural Crop Breeding, Hefei 230036, Anhui, China
  • Received:2025-09-20 Accepted:2025-11-18 Published:2026-03-12 Published online:2025-11-25
  • Contact: *侯华兰, E-mail: hhl@ahau.edu.cn
  • About author:

    **Contributed equally to this work

  • Supported by:
    National Natural Science Foundation of China(32201853);Anhui Provincial Natural Science Foundation(2108085QC122);Natural Science Research Projects of Anhui Province University(KJ2020A0098);Anhui Provincial College Student Innovation and Entrepreneurship Training Program(S202410364070)

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摘要：

UDP-糖基转移酶基因广泛参与植物生长和抗逆性的调节, 是糖基转移酶中最大的一个家族。马铃薯作为我国重要的菜粮兼用型作物, 其生长和产量受到盐胁迫的严重影响。目前, 参与马铃薯盐胁迫响应的UGT基因及其机制尚不明晰。本研究通过分析前期的马铃薯盐胁迫转录组数据, 从马铃薯中克隆得到StUGT52基因, 该基因CDS全长1488 bp, 编码495个氨基酸。蛋白序列分析显示, StUGT52为不稳定的亲水性蛋白, 与番茄SlLS1-like和茄子SpLS1-like的亲缘关系最近。实时荧光定量PCR (RT-qPCR)分析表明, StUGT52基因受盐胁迫显著诱导。StUGT52蛋白主要定位于细胞质和细胞核中。利用农杆菌蘸花法转化拟南芥, 获得7个异源表达StUGT52基因的转基因株系。在盐胁迫处理条件下, 转基因株系种子发芽率和根长均显著高于野生型, Fv/Fm值和可溶性糖含量也显著高于野生型株系, 而离子泄漏率、MDA和O2?累积均显著低于野生型株系, 表明StUGT52基因通过促进渗透调节物质累积, 并抑制活性氧产生, 减轻膜脂氧化损伤, 进而提升转基因拟南芥的耐盐性。研究结果为进一步探究StUGT52基因在马铃薯盐胁迫应答中的功能奠定了理论基础, 为耐盐性作物遗传改良提供了新的基因资源。

关键词: 马铃薯, StUGT52基因, 异源过表达, 耐盐性, 功能分析

Abstract:

UDP-glycosyltransferase (UGT) genes, the largest family of glycosyltransferases, play diverse roles in regulating plant growth and enhancing stress tolerance. Potato (Solanum tuberosum), a vital dual-purpose crop (used both as food and vegetable) in China, suffers significant yield losses under salinity stress. However, the specific UGT genes involved in salt stress responses in potato and their underlying mechanisms remain poorly understood. In this study, StUGT52 was identified through transcriptomic analysis of salt-stressed potato and subsequently cloned. The StUGT52 gene contains a 1488 bp coding sequence encoding 495 amino acids. Protein sequence analysis indicated that StUGT52 is an unstable, hydrophilic protein, and phylogenetic analysis revealed its closest homologs to be SlLS1-like from tomato and SpLS1-like from eggplant. RT-qPCR analysis confirmed that StUGT52 expression is significantly upregulated under salt stress. Subcellular localization analysis showed that the protein predominantly localizes to the cytoplasm and nucleus. Seven independent Arabidopsis thaliana transgenic lines overexpressing StUGT52 were generated via Agrobacterium-mediated floral dip transformation. Under salt stress conditions, transgenic lines exhibited significantly higher seed germination rates and longer root lengths compared to wild-type plants. Additionally, transgenic lines showed increased Fv/Fm ratios and soluble sugar contents, while exhibiting reduced ion leakage, malondialdehyde (MDA) levels, and superoxide anion (O2?) accumulation. These findings demonstrate that StUGT52 enhances salt tolerance in transgenic Arabidopsis by promoting the accumulation of osmoregulatory substances and reducing reactive oxygen species (ROS) production, thereby mitigating membrane lipid peroxidation damage. This study provides novel insights into the role of StUGT52 in potato salt stress adaptation and offers a promising genetic resource for improving salinity tolerance in crops through molecular breeding.

Key words: potato, StUGT52 gene, ectopic expression, salt tolerance, functional analysis

图1

盐胁迫处理下StUGT52的表达模式分析 A: 盐胁迫转录组数据中StUGT52基因表达量; B: RT-qPCR分析盐胁迫处理下StUGT52基因表达量。误差线代表标准误(n = 3)。"

图2

StUGT52系统发育分析及蛋白序列比对 A: StUGT52与不同物种同源蛋白进化关系分析; B: StUGT52与不同物种同源蛋白序列比对; 红色框中序列为保守的UDP-糖基转移酶结构域(PSPG box)。C: PCR扩增StUGT52基因CDS序列。"

图3

StUGT52蛋白的生物信息学分析 A: StUGT52蛋白二级结构; B: StUGT52蛋白三级结构; C: StUGT52蛋白信号肽分析; D: StUGT52蛋白跨膜结构域; E: StUGT52蛋白糖基化位点; F: StUGT52蛋白磷酸化位点。"

图4

StUGT52蛋白亚细胞定位 A: 35S::StUGT52-GFP重组载体结构图; GFP: 绿色荧光蛋白, NOS: 胭脂碱合成酶终止子, CaMV 35S: 花椰菜花叶病毒35S启动子, RB: 右边界, LB: 左边界。B: StUGT52-GFP蛋白亚细胞定位观察。标尺为50 μm。"

图5

StUGT52在不同组织中的表达模式"

图6

StUGT52过表达株系的定量PCR鉴定 WT: 野生型拟南芥; OE1~OE7: StUGT52过表达株系。"

图7

StUGT52过表达植株在盐胁迫条件下的发芽率统计 A: 野生型(WT)和StUGT52过表达株系(OE1和OE2)在0、100、150和200 mmol L-1 NaCl处理下萌发7 d后的表型; B: 野生型(WT)和StUGT52过表达株系(OE1和OE2)在0、100、150和200 mmol L-1 NaCl处理下的发芽率统计。"

图8

盐胁迫条件下StUGT52过表达植株根长分析 野生型(WT)和StUGT52过表达株系(OE1和OE2)在0、100和150 mmol L-1 NaCl处理7 d后的表型(A)和根长测定(B)。*表示显著性差异(P < 0.05, t检验)。"

图9

StUGT52过表达株系在盐胁迫下的生理指标测定 A: 野生型(WT)和StUGT52过表达株系(OE1和OE2)在200 mmol L-1 NaCl处理后的表型; B, C: Fv/Fm值分析; D: 离子泄漏率测定; E: 丙二醛含量; F: 可溶性糖含量; G: NBT染色。**表示在0.01水平差异显著。"

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