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作物学报 ›› 2026, Vol. 52 ›› Issue (6): 1682-1697.doi: 10.3724/SP.J.1006.2026.54138
彭佳泺1(
), 李颖1, 李丹丹1, 杨军宁1, 郭学峰1, 张文姣1, 俞晓雪1, 周亚荣1, 王振玉2, 王彩香1, 马雄风2,*(), 宿俊吉1,*()
Peng Jia-Luo1(), Li Ying1, Li Dan-Dan1, Yang Jun-Ning1, Guo Xue-Feng1, Zhang Wen-Jiao1, Yu Xiao-Xue1, Zhou Ya-Rong1, Wang Zhen-Yu2, Wang Cai-Xiang1, Ma Xiong-Feng2,*(), Su Jun-Ji1,*()
摘要：
侧生器官边界域(lateral organ boundaries domain, LBD)家族基因在植物多种生物学过程中发挥重要作用。前人研究表明, I类LBD基因参与调控植物开花时间, 然而陆地棉I类LBD基因是否具有类似功能尚不清楚。本研究在全基因组水平鉴定并分析陆地棉I类LBD家族成员; 通过单倍型分析筛选调控开花相关候选基因; 利用RNA-seq及RT-qPCR确定候选基因在不同组织和早晚花品种中的表达模式; 通过病毒诱导的基因沉默(virus-induced gene silencing, VIGS)验证目标基因的功能, 并对其进行育种演化分析及分子标记开发。结果显示, 在陆地棉中鉴定出102个I类GhLBDs基因, 这些基因不均匀分布在26条染色体上, 按照进化关系分为6个组别, 同一组别内的LBD成员具有相似的基序组成与排列模式。通过GhLBDs的单倍型与表达分析, 筛选出开花期相关候选基因GhLBD6。通过VIGS技术结合石蜡切片、荧光定量等, 发现抑制GhLBD6表达会导致植株花芽分化提前, 现蕾期和开花期分别显著提前6.57 d和6.86 d。另外, GhLBD6编码区存在2种单倍型: GhLBD6-Hap 1和GhLBD6-Hap 2。其中, GhLBD6-Hap 1为早花的优异等位变异, 在陆地棉育种过程中受到人工选择。最后开发了可用于区分GhLBD6两种单倍型的竞争性等位基因特异性PCR (kompetitive allele-specific PCR, KASP)分子标记。本研究结合正反向遗传学试验结果, 证明了GhLBD6参与陆地棉开花期调控, 为棉花早熟分子育种奠定了基础。
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