欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2026, Vol. 52 ›› Issue (6): 1817-1829.doi: 10.3724/SP.J.1006.2026.55068

• 耕作栽培·生理生化 • 上一篇    下一篇

外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响

金昱何1(), 王雪菲1, 徐张一娃1, 缪怡宁1, 蒋云杰2, 伊莹1, 缪德麟1, 朱静仪1, 钟一帆1, 陈铭亨1, 方芳1, 刘鹏1,*()   

  1. 1 浙江师范大学生命科学学院, 浙江金华 321004
    2 浙江师范大学教育学院, 浙江金华 321004
  • 收稿日期:2025-11-08 接受日期:2026-03-16 出版日期:2026-06-12 网络出版日期:2026-03-20
  • 通讯作者: * 刘鹏, E-mail: sky79@zjnu.cn
  • 作者简介:金昱何, E-mail: 1498942439@qq.com
  • 基金资助:
    国家自然科学基金项目(30540056);国家自然科学基金项目(31971426);国家自然科学基金项目(32001224)

Effects of exogenous hormones on chlorophyll fluorescence parameters and the antioxidant enzyme system in soybean leaves under low-temperature stress

Jin Yu-He1(), Wang Xue-Fei1, Xu Zhang-Yi-Wa1, Miao Yi-Ning1, Jiang Yun-Jie2, Yi Ying1, Miao De-Lin1, Zhu Jing-Yi1, Zhong Yi-Fan1, Chen Ming-Heng1, Fang Fang1, Liu Peng1,*()   

  1. 1 College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
    2 College of Education, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
  • Received:2025-11-08 Accepted:2026-03-16 Published:2026-06-12 Published online:2026-03-20
  • Contact: * Liu Peng, E-mail: sky79@zjnu.cn
  • Supported by:
    National Natural Science Foundation of China(30540056);National Natural Science Foundation of China(31971426);National Natural Science Foundation of China(32001224)

RichHTML

12

PDF

104

摘要：

低温胁迫是制约大豆生产的关键非生物逆境, 为探究外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响, 本试验以耐冷性差异较大的蒙豆16 (Mengdou 16)和黑河18 (Heihe 18)为试验材料, 在4℃低温胁迫下分别喷施最适浓度的脱落酸(ABA)、水杨酸(SA)、褪黑素(MT)、油菜素内酯(BR)和茉莉酸(JA), 探究其对大豆各时期(2、4和6 d)形态形状、光合特性、渗透调节系统和抗氧化酶系统的影响。同时通过隶属函数分析法筛选出高效抗寒激素, 在此基础上采用响应面优化法探究激素最佳配比。结果表明, 大豆在低温胁迫下损伤严重, 蒙豆16的抗寒能力强于黑河18, 生理响应更为稳定。各外源激素处理均能缓解低温对大豆生长的抑制作用, ABA、SA和MT浓度分别为13.93、200.62和30.30 mg L-1时效果最佳, 喷施外源激素对大豆株高、鲜重和叶面积均呈积极作用, 但2个品种的响应程度存在差异。蒙豆16中叶绿素总量的最大增幅为97.60%, 黑河18为108.28%; 2个品种的初始荧光(F0)和非光化学猝灭系数(qN)下降, 蒙豆16的光化学猝灭系数(qP)和光合相对电子传递速率(ETR)上升, 黑河18的qP部分下降, F0最大降幅分别可达34.38%和24.86%, 光合转化效率显著增强。各处理下丙二醛(MDA)含量均显著降低, 蒙豆16的最大降幅为66.50%, 黑河18为62.57%; 脯氨酸含量则有所上升, 最大增幅分别为24.47%和19.97%。此外, 超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均显著增加, SOD与CAT活性在第4天达到最大升幅, POD则在第6天达到最大。综上, 施加不同外源激素可以通过调节光化学系统、抗氧化酶系统和渗透调节系统等途径来缓解低温对大豆造成的损伤, 也为大豆的低温种植提供了理论依据和实践经验。

关键词: 低温胁迫, 外源激素, 大豆, 叶绿素荧光, 抗氧化酶系统

Abstract:

Low-temperature stress is a major abiotic constraint on soybean production. To evaluate how exogenous hormones affect chlorophyll fluorescence and the antioxidant defense system in soybean leaves under low-temperature stress, we used two cultivars with contrasting cold tolerance, Mengdou 16 and Heihe 18. Plants were exposed to 4℃ and treated separately with optimal concentrations of abscisic acid (ABA), salicylic acid (SA), melatonin (MT), brassinolide (BR), and jasmonic acid (JA). Their effects on morphology, photosynthetic performance, osmotic adjustment, and antioxidant enzyme activities were assessed after 2, 4, and 6 days. Membership function analysis was used to identify the most effective hormones for enhancing cold tolerance, and response surface methodology was applied to determine an optimal hormone combination. Low-temperature stress caused substantial injury, with Mengdou 16 showing greater cold tolerance and more stable physiological responses than Heihe 18. All hormone treatments alleviated growth inhibition, and the most effective concentrations were 13.93 mg L-1 ABA, 200.62 mg L-1 SA, and 30.30 mg L-1 JA. Hormone application increased plant height, fresh weight, and leaf area, although responses differed between cultivars. Total chlorophyll content increased by up to 97.60% in Mengdou 16 and 108.28% in Heihe 18. In both cultivars, initial fluorescence (F0) and non-photochemical quenching (qN) decreased; photochemical quenching (qP) and electron transport rate (ETR) increased in Mengdou 16, whereas qP in Heihe 18 decreased in some treatments. The maximum reductions in F0 were 34.38% and 24.86% in Mengdou 16 and Heihe 18, respectively, indicating improved photosynthetic energy conversion. Malondialdehyde (MDA) content decreased significantly under all treatments, with maximum reductions of 66.50% (Mengdou 16) and 62.57% (Heihe 18), while proline content increased by up to 24.47% and 19.97%, respectively. Activities of SOD, POD, and CAT were also enhanced, with SOD and CAT peaking at day 4 and POD showing the greatest increase at day 6. Overall, exogenous hormones mitigated low-temperature damage in soybean by improving photochemical performance and strengthening antioxidant and osmotic adjustment systems, providing a theoretical basis and practical guidance for soybean production under low-temperature conditions.

Key words: low-temperature stress, exogenous hormones, Glycine max (Linn.) Merr, chlorophyll fluorescence, antioxidant enzyme system

表1

不同植物激素对大豆表观性状的影响"

指标
Index		 品种
Variety		 天数
Days (d)		 CK LT SA200 MT30
株高
PH (cm)		 蒙豆16
Mengdou 16 (MD)		 2 23.11±0.11 a 18.07±0.12 d 21.15±0.21 b 20.78±0.20 b
4 25.99±0.10 a 19.56±0.16 e 23.77±0.10 b 22.74±0.22 c
6 28.12±0.05 a 19.78±0.02 f 24.20±0.06 b 23.72±0.02 c
黑河18
Heihe 18 (HH)		 2 22.01±0.10 a 16.74±0.21 e 19.37±0.19 b 18.56±0.20 c
4 24.64±0.25 a 18.47±0.09 f 21.68±0.19 c 20.46±0.16 d
6 26.18±0.04 a 18.77±0.04 g 22.11±0.02 d 22.65±0.01 c
鲜重
FW (g)		 蒙豆16
Mengdou 16 (MD)		 2 5.44±0.03 a 3.33±0.10 g 5.07±0.03 b 4.68±0.02 d
4 6.46±0.06 a 3.60±0.03 f 5.62±0.02 b 5.40±0.01 c
6 7.24±0.04 a 3.82±0.02 g 5.97±0.03 c 5.77±0.03 d
黑河18
Heihe 18 (HH)		 2 7.27±0.10 a 3.78±0.02 f 5.97±0.04 c 5.75±0.04 d
4 8.18±0.08 a 4.21±0.08 e 6.75±0.07 bc 6.56±0.04 c
6 9.33±0.10 a 4.33±0.06 e 7.32±0.04 b 7.08±0.03 c
叶面积
LA (cm2)
 蒙豆16
Mengdou 16 (MD)		 2 31.94±0.09 a 28.56±0.17 c 31.02±0.18 a 30.99±0.09 ab
4 32.63±0.15 a 28.05±0.11 c 30.77±0.14 a 30.07±0.11 ab
6 33.36±0.11 a 27.87±0.03 d 30.27±0.24 bc 29.71±0.22 c
黑河18
Heihe 18 (HH)		 2 35.17±0.22 a 29.08±0.23 c 34.33±0.18 ab 33.69±0.13 b
4 36.17±0.16 a 28.39±0.18 c 33.15±0.13 ab 32.52±0.18 b
6 37.16±0.20 a 27.43±0.22 c 32.19±0.16 ab 31.57±0.22 b
指标
Index		 品种
Variety		 天数
Days (d)		 ABA14 JA30 BR0.12
株高
PH (cm)		 蒙豆16
Mengdou 16 (MD)		 2 21.33±0.11 b 19.52±0.15 c 20.60±0.16 b
4 23.98±0.20 b 20.93±0.26 d 22.48±0.13 c
6 24.63±0.20 b 21.65±0.22 e 22.93±0.17 d
株高
PH (cm)		 黑河18
Heihe 18 (HH)		 2 19.86±0.14 b 17.94±0.09 d 18.38±0.26 cd
4 22.32±0.16 b 19.86±0.16 e 20.15±0.19 de
6 22.81±0.12 b 20.62±0.11 f 21.03±0.03 e
鲜重
FW (g)		 蒙豆16
Mengdou 16 (MD)		 2 4.88±0.05 c 4.08±0.02 f 4.30±0.03 e
4 5.51±0.04 bc 4.65±0.05 e 5.09±0.04 d
6 6.09±0.04 b 5.17±0.03 f 5.31±0.02 e
黑河18
Heihe 18 (HH)		 2 6.37±0.03 b 5.34±0.05 e 5.45±0.03 e
4 6.93±0.03 b 6.05±0.04 d 6.23±0.03 d
6 7.21±0.02 bc 6.38±0.03 d 6.47±0.03 d
叶面积
LA (cm2)		 蒙豆16
Mengdou 16 (MD)		 2 31.03±0.13 a 30.57±0.20 b 30.44±0.25 b
4 30.11±0.15 a 29.70±0.20 b 29.91±0.20 b
6 29.90±0.21 ab 29.44±0.16 c 29.73±0.17 c
黑河18
Heihe 18 (HH)		 2 34.23±0.25 ab 33.74±0.30 b 33.24±0.19 b
4 33.00±0.11 b 32.65±0.22 b 32.21±0.20 b
6 32.03±0.32 b 31.68±0.21 b 31.24±0.20 b

图1

不同植物激素对大豆叶绿素含量的影响 处理和缩写同表1。SPAD value: 叶绿素含量值。不同小写字母表示同一时间点各处理间在P < 0.05水平差异显著。"

表2

不同激素在最适浓度下对低温胁迫下大豆叶绿素荧光参数的影响"

品种
Variety		 指标
Index		 天数
Days (d)		 CK LT SA200 MT30
蒙豆16
Mengdou 16 (MD)		 F0 2 0.281±0.002 d 0.445±0.002 a 0.292±0.002 cd 0.303±0.003 bc
4 0.255±0.002 e 0.414±0.002 a 0.322±0.002 d 0.319±0.001 b
6 0.284±0.002 d 0.470±0.002 a 0.406±0.002 b 0.397±0.002 cd
Fv/Fm 2 0.815±0.002 a 0.792±0.002 cd 0.803±0.002 b 0.796±0.003 bc
4 0.818±0.002 a 0.702±0.002 d 0.767±0.002 b 0.703±0.003 d
6 0.818±0.002 a 0.732±0.002 b 0.646±0.002 e 0.651±0.002 e
qP 2 0.684±0.002 a 0.318±0.002 f 0.412±0.003 c 0.498±0.003 b
4 0.687±0.002 a 0.318±0.002 g 0.406±0.002 d 0.492±0.003 b
6 0.687±0.002 a 0.320±0.003 f 0.398±0.002 d 0.486±0.003 b
qN 2 0.433±0.002 e 0.660±0.002 a 0.457±0.001 c 0.448±0.001 d
4 0.426±0.002 f 0.701±0.002 a 0.478±0.001 c 0.461±0.001 e
6 0.431±0.002 f 0.756±0.001 a 0.492±0.001 d 0.480±0.001 e
ETR 2 23.22±0.06 a 20.04±0.06 e 21.53±0.09 d 21.74±0.15 cd
4 22.81±0.07 a 16.72±0.12 e 19.22±0.12 c 17.41±0.09 d
6 23.03±0.07 a 14.41±0.12 e 16.62±0.12 c 15.72±0.06 d
黑河18
Heihe 18 (HH)		 F0 2 0.261±0.002 e 0.370±0.003 a 0.278±0.002 d 0.281±0.001 cd
4 0.261±0.002 c 0.255±0.002 a 0.414±0.002 b 0.322±0.002 c
6 0.257±0.003 e 0.447±0.001 a 0.372±0.002 bc 0.349±0.001 d
Fv/Fm 2 0.828±0.001 a 0.773±0.002 b 0.778±0.002 b 0.737±0.002 c
4 0.825±0.001 a 0.697±0.001 b 0.689±0.001 b 0.675±0.002 c
6 0.828±0.002 a 0.640±0.002 c 0.638±0.001 c 0.628±0.002 d
qP 2 0.582±0.003 a 0.313±0.003 c 0.393±0.002 b 0.290±0.002 de
4 0.583±0.003 a 0.316±0.003 d 0.410±0.002 c 0.299±0.002 d
6 0.579±0.003 a 0.307±0.002 c 0.377±0.002 b 0.271±0.002 e
qN 2 0.537±0.002 f 0.867±0.002 a 0.592±0.002 e 0.642±0.003 c
4 0.553±0.002 g 0.902±0.002 a 0.618±0.002 f 0.665±0.003 d
6 0.535±0.002 g 0.966±0.002 a 0.633±0.002 f 0.672±0.002 d
ETR 2 20.31±0.10 a 17.51±0.17 d 18.40±0.12 c 19.11±0.10 b
4 20.20±0.09 a 15.12±0.09 d 16.21±0.09 c 17.41±0.12 b
6 20.81±0.09 a 12.92±0.09 e 13.52±0.10 d 13.80±0.09 d
品种
Variety		 指标
Index		 天数
Days (d)		 ABA14 JA30 BR0.12
蒙豆16
Mengdou 16 (MD)		 F0 2 0.308±0.002 bc 0.298±0.002 bc 0.312±0.015 b
4 0.327±0.002 b 0.329±0.001 c 0.319±0.010 b
6 0.410±0.002 b 0.408±0.002 b 0.411±0.002 b
蒙豆16
Mengdou 16 (MD)		 Fv/Fm 2 0.805±0.002 b 0.783±0.002 d 0.760±0.007 e
4 0.742±0.002 c 0.701±0.003 d 0.698±0.007 d
6 0.659±0.002 d 0.626±0.002 f 0.670±0.002 c
qP 2 0.492±0.002 b 0.358±0.002 e 0.380±0.007 d
4 0.481±0.002 c 0.355±0.003 f 0.385±0.007 e
6 0.464±0.002 c 0.371±0.002 e 0.392±0.002 d
qN 2 0.456±0.002 c 0.469±0.002 b 0.473±0.004 b
4 0.472±0.002 d 0.483±0.001 c 0.503±0.003 b
6 0.508±0.002 c 0.519±0.002 b 0.519±0.002 b
ETR 2 21.60±0.15 d 22.52±0.09 b 22.22±0.43 bc
4 19.31±0.09 c 19.91±0.15 b 20.03±0.21 b
6 17.72±0.18 b 17.76±0.07 b 16.60±0.31 c
黑河18
Heihe 18 (HH)		 F0 2 0.286±0.002 c 0.297±0.002 b 0.287±0.004 c
4 0.319±0.001 b 0.327±0.002 b 0.329±0.001 b
6 0.366±0.002 c 0.373±0.002 b 0.348±0.002 d
Fv/Fm 2 0.779±0.001 b 0.748±0.001 c 0.751±0.015 c
4 0.697±0.001 b 0.677±0.002 c 0.676±0.007 c
6 0.669±0.001 b 0.631±0.001 d 0.632±0.001 d
qP 2 0.285±0.003 e 0.229±0.003 f 0.295±0.001 d
4 0.293±0.003 d 0.236±0.002 e 0.298±0.008 d
6 0.284±0.003 d 0.245±0.003 f 0.273±0.003 e
qN 2 0.613±0.002 d 0.690±0.002 b 0.647±0.001 c
4 0.631±0.002 e 0.723±0.003 b 0.675±0.004 c
6 0.641±0.002 e 0.752±0.002 b 0.694±0.002 c
ETR 2 19.20±0.12 b 18.43±0.12 c 19.23±0.47 b
4 17.05±0.09 b 16.53±0.15 c 16.30±0.31 c
6 14.91±0.09 c 14.71±0.12 c 15.21±0.09 b

图2

6 d时不同激素处理下大豆叶片中MDA含量和Pro含量 处理和缩写同表1。MDA: 丙二醛; Pro: 脯氨酸。不同小写字母表示同一品种同一时间点各处理间在P < 0.05水平差异显著。"

图3

外源激素对低温胁迫下大豆叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响 处理和缩写同表1。SOD: 超氧化物歧化酶; POD: 过氧化物酶; CAT: 过氧化氢酶。不同小写字母表示同一品种同一时间点各处理间在P < 0.05水平差异显著。"

表3

低温胁迫下各植物激素对大豆各抗寒指标影响的隶属函数值综合评价"

处理
Treatment		 隶属函数值
Subordinate function value
株高
PH		 鲜重
FW		 叶面积
LA		 叶绿素含量
Chlorophyll content		 F0 Fv/Fm qP qN
SA200 0.86 0.75 0.87 1.00 1.00 0.88 0.49 0.95
MT30 0.71 0.66 0.78 0.77 0.75 0.96 1.00 1.00
ABA14 1.00 0.84 1.00 0.81 0.76 0.82 0.94 0.90
JA30 0.07 1.00 0.76 0.59 0.52 1.00 0.37 0.84
BR0.12 0.50 0.89 0.76 0.76 0.62 0.98 0.40 0.86
处理
Treatment		 隶属函数值
Subordinate function value		 平均值
Average		 排名
Rank
ETR Pro MDA SOD POD CAT
SA200 0.81 0.98 0.89 1.00 0.99 1.00 0.89 2
MT30 0.25 0.81 0.98 0.82 1.00 0.95 0.82 3
ABA14 0.66 1.00 1.00 0.83 0.99 0.98 0.89 1
JA30 1.00 0.88 0.89 0.81 0.99 0.99 0.76 5
BR0.12 0.69 0.83 0.88 0.88 0.99 0.99 0.79 4

图4

复合激素配比响应面优化曲面图 ABA: 脱落酸; SA: 水杨酸; MT: 褪黑素。"

[1] 程爽, ULAANDUU Namuun, 李卓琳, 等. 植物光系统II (PSII)应答非生物胁迫机理研究进展. 生物技术通报, 2023, 39(12): 33-42.
doi: 10.13560/j.cnki.biotech.bull.1985.2023-0658
Cheng S, Namuun U, Li Z L, et al. Research progress in the mechanism of plant photosystem II (PSII) responsing to abiotic stress. Biotechnol Bull, 2023, 39(12): 33-42 (in Chinese with English abstract).
[2] 王芳, 王淇, 赵曦阳. 低温胁迫下植物的表型及生理响应机制研究进展. 分子植物育种, 2019, 17: 5144-5153.
Wang F, Wang Q, Zhao X Y. Research progress of phenotype and physiological response mechanism of plants under low temperature stress. Mol Plant Breed, 2019, 17: 5144-5153 (in Chinese with English abstract).
[3] Kang M, Wang S Y, Xu Z K, et al. Simulating the effects of low-temperature stress during flowering stage on leaf-level photosynthesis with current rice models. Agric For Meteor, 2024, 354: 110087.
doi: 10.1016/j.agrformet.2024.110087
[4] 施文华, 严茂林, 刘昌勇, 等. 我国油料进口贸易的结构特征及对策分析. 中国油脂, 2023, 48(8): 1-8.
Shi W H, Yan M L, Liu C Y, et al. Structural characteristics and countermeasures analysis of Chinese oilseeds import trade. China Oils Fats, 2023, 48(8): 1-8 (in Chinese with English abstract).
[5] 于水, 张晓龙, 王妍, 等. 气候变化对中国东北三省主要粮食作物影响研究综述. 中国生态农业学报(中英文), 2024, 32: 970-985.
Yu S, Zhang X L, Wang Y, et al. Review of research on the impacts of climate change on staple grain crops in the three provinces of Northeast China. Chin J Eco-Agric, 2024, 32: 970-985 (in Chinese with English abstract).
[6] 高红秀, 朱琳, 刘天奇, 等. 水稻植物激素响应低温胁迫反应的转录组分析. 分子植物育种, 2021, 19: 4188-4197.
Gao H X, Zhu L, Liu T Q, et al. Transcriptomic analysis of plant hormone response to low temperature stress in rice. Mol Plant Breed, 2021, 19: 4188-4197 (in Chinese with English abstract).
[7] 盖志佳, 张敬涛, 刘婧琦, 等. 耐低温大豆品种(系)的筛选与研究. 中国种业, 2018(6): 57-60.
Gai Z J, Zhang J T, Liu J Q, et al. Screening and study of soybean varieties (lines) with low temperature tolerance. China Seed Ind, 2018(6): 57-60 (in Chinese).
[8] 许基磊, 汪兴中, 范吉标. 盐胁迫诱导野大豆生理和光合作用的变化. 植物科学学报, 2022, 40: 829-838.
Xu J L, Wang X Z, Fan J B. Changes in physiology and photosynthesis of Glycine soja Sieb. et Zucc. induced by salt stress. Plant Sci J, 2022, 40: 829-838 (in Chinese with English abstract).
[9] 陈金梅, 褚一凡, 谭启洋, 等. 外源脱落酸对湿地植物美人蕉抗寒性的影响. 水生生物学报, 2021, 45: 1264-1272.
Chen J M, Chu Y F, Tan Q Y, et al. Study on increase of the cold resistance and overwintering effect of Canna indica linn in constructed wetland by abscisic acid. Acta Hydrobiol Sin, 2021, 45: 1264-1272 (in Chinese with English abstract).
[10] 周丽霞, 曹红星, 肖勇. 外源水杨酸对低温胁迫椰子幼苗生理特性的影响. 南方农业学报, 2017, 48: 2039-2045.
Zhou L X, Cao H X, Xiao Y. Effects of exogenous salicylic acid on physiological characteristics of Cocos nucifera L. young seedlings under cold stress. J South Agric, 2017, 48: 2039-2045 (in Chinese with English abstract).
[11] Ding F, Liu B, Zhang S X. Exogenous melatonin ameliorates cold-induced damage in tomato plants. Sci Hortic, 2017, 219: 264-271.
doi: 10.1016/j.scienta.2017.03.029
[12] 陈奕可, 代星苗, 田义, 等. 低温胁迫下外源油菜素内酯对核桃抗寒性的影响. 北方园艺, 2025(6): 72-81.
Chen Y K, Dai X M, Tian Y, et al. Effects of exogenous brassinolide on cold resistance of walnut under low-temperature stress. North Hortic, 2025(6): 72-81 (in Chinese with English abstract).
[13] Iqbal A, Bao H N, Wang J, et al. Role of jasmonates in plant response to temperature stress. Plant Sci, 2025, 355: 112477.
doi: 10.1016/j.plantsci.2025.112477
[14] 张雪悦, 左师宇, 田礼欣, 等. 不同密度下越冬型黑麦产量形成的光合特性差异. 草业学报, 2019, 28(3): 131-141.
doi: 10.11686/cyxb2018199
Zhang X Y, Zuo S Y, Tian L X, et al. Effect of planting density on photosynthetic performance and yield of winter rye. Acta Pratac Sin, 2019, 28(3): 131-141 (in Chinese with English abstract).
[15] Liu C, Yu Y C, Liu H Z, et al. Effect of different copper oxide particles on cell division and related genes of soybean roots. Plant Physiol Biochem, 2021, 163: 205-214.
doi: 10.1016/j.plaphy.2021.03.051
[16] 肖强, 郑海雷, 陈瑶, 等. 盐度对互花米草生长及脯氨酸、可溶性糖和蛋白质含量的影响. 生态学杂志, 2005, 24: 373-376.
Xiao Q, Zheng H L, Chen Y, et al. Effects of salinity on the growth and proline, soluble sugar and protein contents of Spartina alterniflora. Chin J Ecol, 2005, 24: 373-376 (in Chinese with English abstract).
[17] 王继庆, 任毅, 时晓磊, 等. 小麦籽粒超氧化物歧化酶(SOD)活性全基因组关联分析. 中国农业科学, 2021, 54: 2249-2260.
doi: 10.3864/j.issn.0578-1752.2021.11.001
Wang J Q, Ren Y, Shi X L, et al. Genome-wide association analysis of superoxide dismutase (SOD) activity in wheat grain. Sci Agric Sin, 2021, 54: 2249-2260 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2021.11.001
[18] Jday A, Ben Rejeb K, Slama I, et al. Effects of exogenous nitric oxide on growth, proline accumulation and antioxidant capacity in Cakile maritima seedlings subjected to water deficit stress. Funct Plant Biol, 2016, 43: 939-948.
doi: 10.1071/FP15363
[19] Wang P J, Xu Z J, Zhang Y, et al. Over-expression of spermidine synthase 2 (SlSPDS2) in tomato plants improves saline-alkali stress tolerance by increasing endogenous polyamines content to regulate antioxidant enzyme system and ionic homeostasis. Plant Physiol Biochem, 2022, 192: 172-185.
doi: 10.1016/j.plaphy.2022.09.025
[20] Wang Z J, Zhang X J, Yang S H, et al. Optimization of the ultrasound-assisted aqueous enzymatic extraction of Pinus koraiensis nuts oil by response surface methodology. Nat Prod Res, 2023, 37: 2602-2607.
doi: 10.1080/14786419.2022.2053969
[21] 孔维平, 毕银丽, 李少朋, 等. 利用高光谱估测干旱胁迫下接菌根菌大豆叶绿素含量. 农业工程学报, 2014, 30(12): 123-131.
Kong W P, Bi Y L, Li S P, et al. Hyperspectral estimation of leaf chlorophyll content in mycorrhizal inoculated soybean under drought stress. Trans CSAE, 2014, 30(12): 123-131 (in Chinese with English abstract).
[22] Nesterenko T V, Tikhomirov A A, Shikhov V N. Physiological (integral) approach to the use of chlorophyll fluorescence parameters of plant leaves. Russ J Plant Physiol, 2022, 69: 53.
doi: 10.1134/S1021443722030116
[23] Zheng S, Su M, Wang L, et al. Small signaling molecules in plant response to cold stress. J Plant Physiol, 2021, 266: 153534.
doi: 10.1016/j.jplph.2021.153534
[24] Guo M X, Zhang X T, Liu J J, et al. OsProDH negatively regulates thermotolerance in rice by modulating proline metabolism and reactive oxygen species scavenging. Rice, 2020, 13: 61.
doi: 10.1186/s12284-020-00422-3
[25] Miya S P, Modi A T, Tesfay S Z, et al. Maize grain soluble sugar and protein contents in response to simulated hail damage. S Afr J Plant Soil, 2018, 35: 377-383.
doi: 10.1080/02571862.2018.1446222
[26] 严锐, 韩延超, 吴伟杰, 等. 水杨酸处理对鲜莲采后品质及抗氧化酶活性的影响. 中国食品学报, 2022, 22(3): 235-245.
Yan R, Han Y C, Wu W J, et al. Effect of salicylic acid treatment on the postharvest quality and antioxidant enzyme activity of fresh Lotus. J Chin Inst Food Sci Technol, 2022, 22(3): 235-245 (in Chinese with English abstract).
[27] 鲍俊杰, 郭广振, 凡超杰, 等. 基于主成分分析和隶属函数分析研究收获期与青贮对甜玉米秸秆饲用品质的影响. 动物营养学报, 2023, 35: 2677-2689.
doi: 10.12418/CJAN2023.250
Bao J J, Guo G Z, Fan C J, et al. Effects of harvest time and silage on forage quality of sweet corn straw based on principal component analysis and membership function analysis. Chin J Anim Nutr, 2023, 35: 2677-2689 (in Chinese with English abstract).
[28] Wu Y Q, Cai X, Tang Y H. Outcomes of low-temperature stress on biological alterations within Pothos (Epipremnum aureum) leaves. Life, 2022, 12: 1432.
doi: 10.3390/life12091432
[29] 杨昊, 谷文文, 方淑梅, 等. 萘乙酸钠与吲哚丁酸钾对大豆苗期低温胁迫的缓解作用. 作物杂志, 2026(2): 180-187.
Yang H, Gu W W, Fang S M, et al. Alleviation effect of sodium naphthylacetate and potassium indolebutyrate on low temperature stress of soybean seedling stage. Crops, 2026(2): 180-187 (in Chinese with English abstract).
[30] Liu T, Han Y Q, Shi J L, et al. Abscisic acid involved in trehalose improved melon photosynthesis via regulating oxidative stress tolerance and cell morphology structure under cold stress. Environ Exp Bot, 2022, 202: 105042.
doi: 10.1016/j.envexpbot.2022.105042
[31] Dong Z F, Zhao J, Ji W W, et al. Classification of tomato seedling chilling injury based on chlorophyll fluorescence imaging and DBO-BiLSTM. Front Plant Sci, 2024, 15: 1409200.
doi: 10.3389/fpls.2024.1409200
[32] 赵丝雨, 王思宇, 王文艳, 等. 低温和荫蔽双重胁迫对大豆光合特性的影响. 西南大学学报(自然科学版), 2024, 46(8): 22-30.
Zhao S Y, Wang S Y, Wang W Y, et al. The effects of dual stresses of low temperature and shade on the photosynthetic characteristics of soybean. J Southwest Univ (Nat Sci Edn), 2024, 46(8): 22-30 (in Chinese with English abstract).
[33] Guan Y L, Hwarari D, Korboe H M, et al. Low temperature stress-induced perception and molecular signaling pathways in plants. Environ Exp Bot, 2023, 207: 105190.
doi: 10.1016/j.envexpbot.2022.105190
[34] Li X N, Tan D X, Jiang D, et al. Melatonin enhances cold tolerance in drought-primed wild-type and abscisic acid-deficient mutant barley. J Pineal Res, 2016, 61: 328-339.
doi: 10.1111/jpi.12350 pmid: 27299847
[35] Ding F, Ren L M, Xie F, et al. Jasmonate and melatonin act synergistically to potentiate cold tolerance in tomato plants. Front Plant Sci, 2022, 12: 763284.
doi: 10.3389/fpls.2021.763284
[36] 张慧君, 梁亚灵. 火龙果抗寒性研究. 基因组学与应用生物学, 2017, 36: 2088-2093.
Zhang H J, Liang Y L. Study on cold resistance of pitaya. Genom Appl Biol, 2017, 36: 2088-2093 (in Chinese with English abstract).
[37] 潘凌云, 马家冀, 李建民, 等. 植物盐胁迫应答转录因子的研究进展. 生物工程学报, 2022, 38: 50-65.
Pan L Y, Ma J J, Li J M, et al. Advances of salt stress-responsive transcription factors in plants. Chin J Biotechnol, 2022, 38: 50-65 (in Chinese with English abstract).
[38] Wang S Q, Dai H P, Skuza L, et al. Difference in Cd2+ flux around the root tips of different soybean (Glycine max L.) cultivars and physiological response under mild cadmium stress. Chemosphere, 2022, 297: 134120.
doi: 10.1016/j.chemosphere.2022.134120
[39] 贾景瑞, 褚霈宇, 裴福月, 等. 色氨酸对大豆幼苗低温胁迫抗性的影响. 分子植物育种, 网络首发[2025-05-20], https://link.cnki.net/urlid/46.1068.S.20250520.1503.004.
Jia J R, Zhu P Y, Pei F Y, et al. Effects of tryptophan on low antioxidant physiology of soybean seedlings under low temperature stress. Mol Plant Breed, Published online [2025-05-20], https://link.cnki.net/urlid/46.1068.S.20250520.1503.004 (in Chinese with English abstract).
[40] Li C, Wang J H, Yu C, et al. Growth-promoting effects of phytohormones on capillary-driven attached Chlorella sp. biofilm. Bioresour Technol, 2022, 364: 128117.
doi: 10.1016/j.biortech.2022.128117
[41] Ding Z H, Wu C L, Tie W W, et al. Strand-specific RNA-seq based identification and functional prediction of lncRNAs in response to melatonin and simulated drought stresses in cassava. Plant Physiol Biochem, 2019, 140: 96-104.
doi: 10.1016/j.plaphy.2019.05.008
[1] 陈檬瑶, 陈强, 史晓蕾, 索文英, 张孟臣, 杨春燕, 闫龙, 张锴, 孟庆民. 大豆籽粒油分含量QTL定位及KASP标记开发[J]. 作物学报, 2026, 52(7): 1985-1996.
[2] 马晨铭, 哈斯图亚, 常泓博, 梁擎天, 游乐, 吴尚蓉. 基于叶绿素荧光成像系统的马铃薯叶片叶绿素含量估测[J]. 作物学报, 2026, 52(7): 2193-2206.
[3] 刘峰, 董宏伟, 张越, 李冉, 孙若男, 刘佳城, 孙树杰, 张旭东, 韩清芳. 玉豆间作减氮稳产的光合机制：光合产物转化效率提升[J]. 作物学报, 2026, 52(7): 2095-2108.
[4] 唐宽强, 李公允, 宋美毅, 赵雪, 常春玲. 大豆株高性状全基因组关联分析及预测模型构建[J]. 作物学报, 2026, 52(6): 1743-1756.
[5] 姚术, 郭凯悦, 翟慧慧, 姚佳慧, 邓文琪, 闫玲, 黄驰, 高阳, 俞嫣然, 赵振邦, 李英慧, 王晓波, 李佳佳. 大豆苗期耐低铁综合评价及优异种质筛选[J]. 作物学报, 2026, 52(5): 1373-1387.
[6] 徐苗苗, 邸太妹, 王洁, 吴叶蝶, 刘恩贝, 王玉春, 王新超, 王璐. 外源槲皮素增强茶树抗寒性的分子机制[J]. 作物学报, 2026, 52(5): 1418-1429.
[7] 张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493.
[8] 马娟娥, 姚有华, 姚晓华, 吴昆仑, 崔永梅. 青稞HvERF039基因的克隆及功能研究[J]. 作物学报, 2025, 51(9): 2341-2357.
[9] 贺红利, 张雨涵, 杨静, 程云清, 赵杨, 李星诺, 司洪亮, 张兴政, 杨向东. 大豆e1-as基因突变体的创制及生理分析[J]. 作物学报, 2025, 51(8): 2228-2239.
[10] 王克晶, 李向华. 我国珍稀的大豆属多年生烟豆和短绒野大豆物种遗传资源濒危性评估分析[J]. 作物学报, 2025, 51(8): 2009-2019.
[11] 孟然, 李赵嘉, 冯薇, 陈悦, 刘路平, 杨春燕, 鲁雪林, 王秀萍. 大豆不同生育时期耐盐性综合评价及耐盐种质筛选[J]. 作物学报, 2025, 51(8): 1991-2008.
[12] 胡蒙, 沙丹, 张晟瑞, 谷勇哲, 张世碧, 李静, 孙君明, 邱丽娟, 李斌. 大豆分枝数QTL定位及候选基因筛选[J]. 作物学报, 2025, 51(7): 1747-1756.
[13] 王琼, 邹丹霞, 陈兴运, 张威, 张红梅, 刘晓庆, 贾倩茹, 魏利斌, 崔晓艳, 陈新, 王学军, 陈华涛. 大豆开花时间和成熟期性状全基因组关联分析与候选基因预测[J]. 作物学报, 2025, 51(6): 1558-1568.
[14] 殷丛丛, 李睿琦, 岳霈尧, 李晨, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 基于闭合哑铃介导等温扩增可视化检测大豆花叶病毒SC15方法的建立及应用[J]. 作物学报, 2025, 51(5): 1248-1260.
[15] 侯天钰, 杜孝敬, 赵志强, 热依木·艾尼瓦尔, 伊达耶图拉·阿不拉, 布哈丽且木·阿不力孜, 袁杰, 张燕红, 王奉斌. 粳稻品种芽期耐冷性评价及耐冷种质筛选[J]. 作物学报, 2025, 51(3): 812-822.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 李绍清, 李阳生, 吴福顺, 廖江林, 李达模. 水稻孕穗期在淹涝胁迫下施肥的优化选择及其作用机理[J]. 作物学报, 2002, 28(01): 115 -120 .
[2] 王兰珍;米国华;陈范骏;张福锁. 不同产量结构小麦品种对缺磷反应的分析[J]. 作物学报, 2003, 29(06): 867 -870 .
[3] 杨建昌;张亚洁;张建华;王志琴;朱庆森. 水分胁迫下水稻剑叶中多胺含量的变化及其与抗旱性的关系[J]. 作物学报, 2004, 30(11): 1069 -1075 .
[4] 袁美;杨光圣;傅廷栋;严红艳. 甘蓝型油菜生态型细胞质雄性不育两用系的研究Ⅲ. 8-8112AB的温度敏感性及其遗传[J]. 作物学报, 2003, 29(03): 330 -335 .
[5] 王永胜;王景;段静雅;王金发;刘良式. 水稻极度分蘖突变体的分离和遗传学初步研究[J]. 作物学报, 2002, 28(02): 235 -239 .
[6] 王丽燕;赵可夫. 玉米幼苗对盐胁迫的生理响应[J]. 作物学报, 2005, 31(02): 264 -268 .
[7] 田孟良;黄玉碧;谭功燮;刘永建;荣廷昭. 西南糯玉米地方品种waxy基因序列多态性分析[J]. 作物学报, 2008, 34(05): 729 -736 .
[8] 胡希远;李建平;宋喜芳. 空间统计分析在作物育种品系选择中的效果[J]. 作物学报, 2008, 34(03): 412 -417 .
[9] 王艳;邱立明;谢文娟;黄薇;叶锋;张富春;马纪. 昆虫抗冻蛋白基因转化烟草的抗寒性[J]. 作物学报, 2008, 34(03): 397 -402 .
[10] 郑希;吴建国;楼向阳;徐海明;石春海. 不同环境条件下稻米组氨酸和精氨酸的胚乳和母体植株QTL分析[J]. 作物学报, 2008, 34(03): 369 -375 .
京ICP备15008789号-2