全基因组分析PEG胁迫下水稻根系转录因子表达变化

doi:10.3724/SP.J.1006.2009.01030

摘要/Abstract

摘要：

转录因子在植物抗逆境中起重要的调节作用。本文使用Affymetrix水稻60K芯片全基因组研究PEG胁迫时2个耐旱性不同的水稻品种的转录因子及转录因子家族的变化，结果表明，在PEG胁迫下，耐旱品种湘丰早119根系共有95个转录因子转录本与对照处理比较表达发生变化(24个为转录水平下调表达, 71个为转录水平上调表达)；干旱敏感品种爱华5号根系有129个转录因子转录本表达发生变化(转录水平上调的转录因子转录本60个，转录水平下调的转录因子转录本69个)；2个品种PEG胁迫响应转录因子隶属的转录因子家族都为30个，但各转录因子所属的30个家族并不完全相同；PEG胁迫逆境中，PEG胁迫响应转录因子转录本表现出品种特异性，湘丰早119有72个为特异响应转录本，爱华5号有106个特异响应的转录因子转录本；2个品种在干旱胁迫下响应的转录因子有23个为重叠转录本，其中有16个在转录水平上调表达，7个在转录水平下调表达；2个品种的PEG胁迫响应转录因子基因在染色体上的分布不同，重叠转录因子基因主要位于第2染色体0.432~26.139 Mb和第5染色体0.076~20.597 Mb之间。

关键词: 水稻, 根系, 基因芯片, 转录因子, 耐旱性差异

Abstract:

Transcription factors play an important role in plant stress tolerance. The Affymetrix rice genome arrays were used to research the expression change of transcription factors and their families in two rice cultivars with different levels of drought tolerance under PEG osmotic stress. Results showed that a total of 95 transcripts encoding transcription factors (24 transcripts were down-regulated and 71 transcripts were up-regulated at the transcription level) in Xingfengzao 119 and 129 transcripts (69 transcripts were down-regulated and 60 transcripts were up-regulated at the transcription level) in Aihua 5 were affected by PEG osmotic stress. Transcription factors transcripts in response to PEG osmotic stresses were belonged to 30 transcription factors families for each of two cultivars, but there was a difference between the two 30 families. The transcription factors transcripts for expressed cultivar specific-response to PEG osmotic stress, with 72 transcripts for Xingfengzao 119 and 106 for Aihua 5. There was an overlap of transcripts in response to PEG osmotic stress between Xingfengzao 119 and Aihua 5, with the 16 and 7 up-regulated and the down-regulated at the transcription level, respectively. Distribution of PEG osmotic stress regulating genes of two cultivars on rice chromosome was different and their transcripts overlapped were located in 0.432 to 26.139 Mb of chromosome 2 and 0.076 to 20.597 Mb of chromosome 5.

Key words: Rice, Root system, Microarray, Transcription factors, Different drought tolerance

马廷臣，陈荣军，余蓉蓉，曾汉来，张端品. 全基因组分析PEG胁迫下水稻根系转录因子表达变化[J]. 作物学报, 2009, 35(6): 1030-1037.

MA Ting-Chen,CHEN Rong-Jun,YU Rong-Rong,ZENG Han-Lai,ZHANG Duan-Pin. Global Genome Expression Analysis of Transcription Factors under PEG Osmotic Stress in Rice Root System[J]. Acta Agron Sin, 2009, 35(6): 1030-1037.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2009.01030

https://zwxb.chinacrops.org/CN/Y2009/V35/I6/1030

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