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作物学报 ›› 2025, Vol. 51 ›› Issue (5): 1400-1408.doi: 10.3724/SP.J.1006.2025.44145
李文佳1,2(
), 廖泳俊2, 黄璐2, 鲁清2, 李少雄2, 陈小平2, 金晶炜1,*(), 王润风2,*()
LI Wen-Jia1,2(), LIAO Yong-Jun2, HUANG Lu2, LU Qing2, LI Shao-Xiong2, CHEN Xiao-Ping2, JIN Jing-Wei1,*(), WANG Run-Feng2,*()
摘要：
开花时间是评价花生早熟品种的重要指标之一, 对产量亦有重要影响。挖掘花生开花时间相关遗传位点并筛选候选基因, 对于花生早熟育种至关重要。本文以390份花生栽培种自然群体为材料, 统计了在5种环境下的开花时间。表型统计结果显示, 5种环境下, 花生开花时间的变异系数介于3.21%~8.54%之间, 存在较丰富的表型变异。花生的开花时间基本呈现正态分布, 且受环境影响较大。通过全基因组关联分析, 共得到259个与开花时间显著关联的SNP位点, 其中有29个位点在2个以上环境中重复出现, 分别位于A01、A02、A03、A04、A05、A07、A10、B01、B02、B03、B04、B06、B07、B09染色体上, 可解释3.47%~8.58%的表型变异。在29个重复出现位点上下游100 kb的置信区间寻找候选基因, 共发现159个有功能注释的基因。在重复检测的位点的候选区间中预测到7个与花生开花时间相关的候选基因, 分别编码R2R3-MYB转录因子、叶绿素a-b结合蛋白、bHLH转录因子、WRKY转录因子、FAR1转录因子。研究结果可为解析花生开花调控遗传机制及花生早熟育种奠定基础。
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