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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (09): 1557-1562.doi: 10.3724/SP.J.1006.2008.01557

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Production and Characterization of Intergeneric Hybrids between Bras-sica Species (B. juncea, B. nigra) and Orychophragmus violaceus by Ovary Culture

WANG Ai-Yun1,LI Xun2,HU Da-You3   

  1. 1 College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, Hunan; 2 Oilseed Research Insti-tute, Hunan Agricultural University, Changsha 410128, Hunan; 3 Yiyang Vocational and Technical College, Yiyang 413039, Hunan, China
  • Received:2008-01-11 Revised:1900-01-01 Online:2008-09-12 Published:2008-09-12
  • Contact: WANG Ai-Yun

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Abstract: Intergeneric hybridization is an available way to create new germplasm and enrich breeding materials. The reciprocal crosses have been made between Brassica species and Orychophragmus violaceus with the objective of carrying out desirable genes transfer from O. violaceus to B. juncea by ovary culture technique. The ovaries were cultured on 6 different culture media, and great differences on the intergeneric crossability were found among cross combinations, media and female parents. Hybrid plants were obtained through ovary culture, 9 of which were similar to female parent B. juncea or the intermediate type between male and female parents in morphology. Only one plant from Younensi × O. violaceus was completely male sterile, and showed significant variation for branching pattern and leaf morphology. Morphological and SSR molecular identifications showed these hybrids were truly intergeneric hybrids.

Key words: Brassica juncea, Orychophragmus violaceus, Intergeneric hybrid, Ovary culture

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