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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (10): 1909-1915.doi: 10.3724/SP.J.1006.2009.01909

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Forms and Accumulation of Aluminum in Tea Plant(Camellia sinensis)

SUN Ting1,LIU Peng1,*,ZHENG Ren-Wei1,2,XIE Zhong-Lei3,LUO Hong1   

  1. 1Key Laboratory of Botany,Zhejiang Normal University,Jinhua 321004,China;2Department of Chemistry,Zhejiang Normal University,Jinhua321004,China;3College of Entironment and Resource,Jilin University,Changchun 130026,China
  • Received:2009-02-18 Revised:2009-07-23 Online:2009-10-12 Published:2009-09-10
  • Contact: LIU Peng, E-mail: sky79@zjnu.cn; Tel: 13566780990

Abstract:

Aluminum toxicity is a major limiting factor affecting yield and quality of crops in acid soil. Camellia sinensis is an Al-accumulating plant grown healthily even in strong acid soil with high aluminum content. The study of the forms and accumulation of aluminum will be helpful to reveal the Al-toleranting mechanism of tea plant. In this paper, the content of Al3+ in tea plants was determined after Camellia sinensis wastreated for 50 d with different concentrations of Al3+ in culture solution. Besides, the forms of Al were analyzed in the roots, stems and leaves of tea plants by the nondestructive 27Al NMR with high accuracy. The old leaves of tea plant were the major Al-accumulating organ, whereas, roots would take the position of it and accumulate more aluminum when the plant was treated with 2 mmol L-1 Al3+. 27Al NMR test showed that the dissoluble aluminum in tea root, stem and leaf existed mainly in the form of [AlO4Al12(OH)24(H2O)12]7+(Al13), whose chemical shift was 63×10-6, and it was the first discovery in tea plant. Besides of Al13, there were two weak absorption peaks of 0.38×10-6 and 0.17×10-6 in three organs tested, which wereundetected aluminum complexes. Other three complexes appeared in old leaf when treated by 5 mmol L-1 Al3+, which were Al-oxalate (1:1), Al-oxalate (1:2), and Al-phosphate, and the chemical shifts were respectively 6.4×10-6, 12.7×10-6 and –6.2×10-6. In summary, most of Al3+in tea plant exist in chelate complexes or organic matter, which can be considered as one of Al-accumulating and Al-tolerance mechanism of tea plant.

Key words: 27aluminum NMR, Camellia sinensis, Aluminum accumulation


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