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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2000-2007.doi: 10.3724/SP.J.1006.2009.02000

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Application of a Functional Marker for Wide Compatibility Gene S5-n of Rice

YANG Jie,WANG Jun,CAO Qing,CHEN Zhi-De,ZHONG Wei-Gong*   

  1. Institute of Food Crops，Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center，Nanjing 210014，Jiangsu，China
  • Received:2009-01-20 Revised:2009-04-27 Online:2009-11-12 Published:2009-08-07
  • Contact: ZHONG Wei-Gong,E-mail: wgzhong0503@yahoo.com.cn; Tel: 025-84390320

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Abstract:

Wide compatibility varieties (WCVs) with S5-n are able to produce highly fertile hybrids when crossed to both indica and japonica varieties. The conventional breeding strategy of pyramiding S5-n is to test the fertility of F1 hybrid. However, this method is labour-consuming, time-consuming and. difficult in operation. The technically simplified approach of marker-assisted selection(MAS) has directly and practically used in rice breeding. Therefore, it is urgent to find a convenient and efficient way in WCV breeding with MAS. The wide compatibility gene (S5-n) has 136 bp deletion compared with the indica and japonica S-5 alleles. In order to improve the precision of marker-assisted selection for S5-n, we developed the functional marker S5136 based on the deletion in the DNA sequence of S5-n. The differentiation for S5-n, S5-i and S5-j in 24 accessions of weedy rice from the district of Yangtze and Huaihe Rivers, 27 accessions of common wild rice (O. rufipogon Griff.) from China, 554 cultivars and genetic resources (O. sativa L.) and 20 breeding lines was investigated by S5136．The results indicated that this marker was low-cost, robust technique that can be utilized to identify the S5-n in WCVs like 02428, Dular and CPSLO17. The S5136 could distinguish the homozygous S5-n/S5-n, homozygous S5-i/S5-i, and heterozygous S5-n/S5-i individuals in F2 population derived from 3037 and 02428. From the tested materials, 13 germplasms with S5-n were identified and conformed by sequencing the S5136 PCR products, and 2 restore lines with S5-n developed from the offspring of 02428 were identified. Also, the compatibility of Kasalath was tested with some indica-japonica hybrids. Therefore, S5136 is a powerful molecular marker in S5-n germplasm screening, perfect marker-assisted selection breeding and seed purity testing for the two line hybrids with Pei’ai 64S as maternal parent.

Key words: Wide compatibility gene S5-n, Functional marker, Oryza sativa L., O.rufipogen Griff., O.sativa f.spontanea

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