Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (07): 1075-1083.doi: 10.3724/SP.J.1006.2010.01075

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Expression Analysis of ZmERD16,a Ubiquitin Extension Protein Gene in Maize (Zea mays L.)

LU Yue-Shang1,2,LIU Ying-Hui2,3,**, ZHANG Deng-Feng2, SHI Yun-Su2, SONG Yan-Chun2, WANG Tian-Yu2,*, YANG De-Guang1,*, LI Yu2   

  1. 1College of Agronomy,Northeast Agricultural University,Harbin 150030,China;2 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3Hebei North University,Zhangjiakou 075000,China
  • Received:2010-01-05 Revised:2010-03-04 Online:2010-07-12 Published:2010-04-28
  • Contact: WANG Tian-Yu,E-mail: wangtianyu@263.net;YANG De-Guang,E-mail: ydgl@tom.com

Abstract: Ubiquitin extension protein is a fusion prot0ein of an ubiquitin monomer followed by a ribosomal protein with 52–53 animo acids or 76–78 amino acids. Ubiquitin extension proteins have been paid attention that they play important roles in responding to stresses and regulating certain developmental processes in plants. We describe here the isolation, sequence characteristics and expression analysis of ZmERD16 gene encoding a homologue of ubiquitin extension protein. ZmERD16 includes a 390 bp open reading frame (ORF) encoding an ubiquitin monomer followed by 53 animo acids, with a predicted molecular mass of 14.7582 kD and pI of 9.94. The genomic DNA and the promoter region of ZmERD16 were obtained by PCR method. The genomic DNA was composed of four exons and three introns. Promoter had some motifs that were related to light, stress, defense, development, auxin and other stresses. The tissue-specific expression analysis suggested that ZmERD16 was constitutively expressed in maize different tissues. Quantitative real-time RT-PCR results showed ZmERD16 was a multiple stresses inducible gene, induced by various stresses, such as salt, dehydration, cold, heat, PEG, methy jasmonat (MJ) and salicylic acid (SA), but not by ABA and 2,4-D. These results suggested that ZmERD16 might play an important role in various signal transduction pathways of stresses in plant.

Key words: Maize, Ubiquitin extension protein, Clone, Stress, ZmERD16

    [1]      Jones A M, Vierstra R D, Daniels S M, Quail P. The role of separate molecular domains in the structure of phytochrome from etiolated Avena sativa L. Planta, 1985, 164: 501–506    
[2]      Dong F-C(董发才), Song C-P(宋纯鹏). The ubiquitin and its physiological functions in plants. Plant Physiol Commun (植物生理学通讯), 1999, 35(1): 54–59 (in Chinese)    
[3]      Wang G-H(王高鸿), Huang J-C(黄久常). Selected degradation of proteins. Chin Bull Life Sci (生命科学), 1999, 11(1): 24–26 (in Chinese with English abstract)    
[4]      Monia B P, Ecker D J, Crooke S T. New perspectives on the structure and function of ubiquitin. Nat Biotechnol, 1990, 8: 209–215    
[5]      Ozkaynak E, Finley D, Solomon M J, Varshavsky A. The yeast ubiquitin genes: a family of natural gene fusions. EMBO J, 1987, 6: 1429–1439    
[6]      Baker R T, Board P G. The human ubiquitin-52 amino acid fusion protein gene shares several structural features with mammalian ribosomal protein genes. Nucl Acids Res, 1991, 19: 1035–1040    
[7]      Callis J, Raasch J A, Vierstra R D. Ubiquitin extension protein of Arabidopsis thealiana. J Biol Chem, 1990, 26: 12486–12493    
[8]      Finley D, Bartel B, Varshavsky A. The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis. Nature, 1989, 338: 394–401    
[9]      Tytgat T, Vanholme B, Meutter J D, Claeys M, Couvreur M, Vanhoutte I, Gheysen G, Criekinge W V, Borgonie G, Coomans A, Gheysen G. A new class of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in plant parasitic cyst nematodes. Mol Plant-Microbe Interact, 2004, 17: 846–852  
[10]      Belknap W R, Garbarino J E. The role of ubiquitin in plant senescence and stress responses. Trends Plant Sci, 1996, 1: 331–335  
[11]      Dreher K, Callis J. Ubiqutin, hormones and biotic stress in plants. Ann Bot(Lond), 2007, 99: 787–822  
[12]      Kiyosue T, Kazuko Y S, Shinozaki K. Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) in Arabidopsis thaliana L.: identification of three ERDs. Plant Mol Biol, 1994, 25: 791–798  
[13]      Li H Y, Wang T Y, Shi Y S, Fu J J, Song Y C, Wang G Y, Li Y. Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). DNA Sequence, 2007, 18: 445–460  
[14]      Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCTmethod. Methods, 2001, 25: 402–408  
[15]      Xu H-T(涂洪涛), An S-H(安世恒), Guo X-R(郭线茹), Luo M-H(罗梅浩), Wu S-Y(吴少英), Yuan G-H(原国辉). Cloning of ubiquitin extension protein gene from Helicoverpa assulta and its expression in Escherichia coli. J Agric Biotechnol (农业生物技术学报), 2006, 14(6): 884–888 (in Chinese with English abstract)  
[16]      Chen W(陈文), Zheng P-P(郑萍萍), Nie L-W(聂刘旺). Construction of testis cDNA library and sequence analysis of ubiquitin/L40e extension gene in Bufobufo gargarizans. Chin J Zoology (动物学杂志), 2007, 42(1): 20–28 (in Chinese with English abstract)  
[17]      Berg J M. Potential metal-binding domains in nucleic acid binding proteins. Science, 1986, 232: 485–487  
[18]      Wei S-S(韦双双), Zhang Y-X(张英霞), Li W-H(李文辉), Zhang Y(张云). Molecular cloning and comparison of ubiquitin fusion protein and ribosomal protein L30 from Ophiophagus Hannah. Zoological Research (动物学研究), 2005, 26 (4): 397–403 (in Chinese with English abstract)  
[19]      Gausiong K, Barkardottir R. Structure and expression of ubiquitin genes in higher plants. Eur J Biochem, 1986, 258: 57–62 Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol, 2006, 141: 391–396
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