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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (09): 1605-1609.doi: 10.3724/SP.J.1006.2010.01605

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Cloning and Activity Analysis of Zea mays ZmPR4 Promoter in Wheat Immature Embryonic Cali

WANG Ai-Yun1,2, ZHUANG Hong-Tao2,3,**,ZHANG Zeng-Yan2,*,ZHANG Xue-Wen3,DU Li-Pu2,YE Xing-Guo2   

  1. 1 College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;     3 College of Life Science and Technology, Hunan Agricultural University, Changsha 410128, China
  • Received:2010-05-04 Revised:2010-07-19 Online:2010-09-12 Published:2010-08-25
  • Contact: ZHANG Zeng-Yan, E-mail: zhangzy@mail.caas.net.cn

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Abstract: The ZmPR4 promoter was cloned from genomic DNA of maize inbred line B37 through specific PCR amplification. The promoter cloned had the same sequence as the fragment registered in GenBank under the accession number of AJ969166. Expression vectors driven by ZmPR4 promoter or Ubiquitin promoterwere constructed, which harbor GUS gene. The vectors were bombarded into wheat immature embryogenic calli. Histochemical assays of GUS activity, the ZmPR4 promoter was obviously expressed in wheat immature embryonic calli, but the expression activity was lower than that of Ubi Promoter. The ZmPR4 promoter could be induced by infection of Rhizoctonia cerealis. PCR assay confirmed the expression of GUS gene driven by ZmPR4 promoter. These results suggest that ZmPR4promoter has a use potential in molecular breeding of resistance in wheat.

Key words: Inducible promoter, Cloning, Calli, Transient expression

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