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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (01): 17-21.doi: 10.3724/SP.J.1006.2014.00017

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Association of puroindoline b-2 Alleles with Yield-Related Traits in Bread Wheat

CHEN Feng,LI Xiang-Nan,CAO Ying-Ying,SUN Jian-Xi,ZHANG Fu-Yan,DONG Zhong-Dong,CUI Dang-Qun*   

  1. Agronomy College / Collaborative Innovation Center of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2013-06-13 Revised:2013-09-16 Online:2014-01-12 Published:2013-10-22
  • Contact: 崔党群, E-mail: cdq62@sohu.com, Tel: 0371-63558537

Abstract:

puroindoline b-2 (Pinb-2) genes were recently discovered and possess high similarity with puroindoline b gene. We have identified several Pinb-2 alleles in B genome of bread wheat (Triticum aestivum L.) and found association between allelic variation on Pinb-2 and grain texture in soft wheat. In this study, we analyzed the association between the Pinb-2 alleles and yield-related traits and flag leaf size, and mapped the Pinb-2 locus using a recombinant inbred line (F8) population. Among the four Pinb-2 genotypes, Pinb-2v3b genotype possessed the highest 1000-grain weight, grain diameter, grain number per spike, grain weight per spike, and grain weight per plant. Therefore, Pinb-2v3b genotype possibly has more preferable yield-related traits than Pinb-2v2, Pinb-2v3a, and Pinb-2v3c genotypes. Additionally, Pinb-2v3a genotype possessed the highest flag leaf width, length, and area. Genetic mapping indicated that Pinb-2 was located on the long arm of chromosome 7B close to SSR marker Barc315. This result provides basic information for use of puroindoline b-2 genes in molecular genetics research and breeding.

Key words: Bread wheat, puroindoline b-2 genes, Yield-related traits, Flag leaf size, Genetic mapping

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