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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (02): 313-319.doi: 10.3724/SP.J.1006.2014.00313

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Regeneration Capacity and Some Affecting Factors of Different Parts of Young Seedlings of Maize (Zea mays L.)

MA Hai-Zhen,ZHU Wei-Wei,WANG Qi-Bai,WANG Guo-Liang,LI Xin-Zhen*,QI Bao-Xiu*   

  1. State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2013-04-11 Revised:2013-08-16 Online:2014-02-12 Published:2013-12-03
  • Contact: 李新征,E-mail: lxz@sdau.edu.cn; 亓宝秀,E-mail: qbx126@sdau.edu.cn

Abstract:

Currently, immature embryo is the most widely used explant for maize regeneration and transformation. However, this material source is restricted by geographical condition, developmental stages and the growing season. Here, we isolated different parts the of three-day-old seedlings to study their regeneration capacity, aiming to establish an alternative efficient regeneration system for maize. Using the shoot tips and primary root of three-day-old seedlings of maize germinated in dark and light as explants, the effects of illumination, genotype, and different concentration of Ca2+ in the induction medium on the primary callus induction rate and seedling differentiation rate were analysed. The result showed that shoot tips and the root tips derived from seedlings germinated in the dark were better source materials. Root materials isolated from the mature zone of the primary root could not be weed to induce primary callus. The primary callus induction rate was the highest when 5 mmol L-1 of Ca2+ was added in the induction medium. The callus induction and differentiation rates of both explants from the six different genotypes were very different. However, the effect of the genotype on primary callus induction and differentiation rates from the two explants was consistent. The differentiation rate and the morphology of the callus from the two explants of the same genotype were also similar. From above, we propose that the 3-day-old seedlings of Qi319 and Luyuan 92 germinated in the dark have the potential to replace the immature embryos for maize tissue culture in the future.

Key words: Maize (Zea mays L.) seedling, Shoot tips, Root tips, Callus induction, Differentiation

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