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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (07): 1296-1303.doi: 10.3724/SP.J.1006.2014.01296

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Improvement of Hybridization Success Rate in Soybean from Northeast China during Winter Nursing in Hainan Island

ZHANG Yong1,2,**,SUN Shi1,**,YANG Xing-Yong2,SUN Xue-Gang1,WU Cun-Xiang1,HAN Tian-Fu1,*   

  1. Agricultural Sciences, Beijing 100081, China; 2 Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Keshan 161606, China
  • Received:2013-12-19 Revised:2014-04-16 Online:2014-07-12 Published:2014-05-19
  • Contact: 韩天富, E-mail: hantianfu@caas.cn, Tel: 010-82105875

Abstract:

 

To accelerate the soybean breeding progress and to broaden the genetic basis of new varieties, it is necessary to improve the hybrid seed-setting rate of soybean during winter nursing. Seven early-maturing soybean varieties from northeast China were selected in hybridization experiments with photoperiod treatments in Sanya, Hainan, China. Morphological traits, growth and development periods, floral organ characteristics and pollen fertility were observed or documented under long day (LD, 18 h) treatment and normal short day (NSD) conditions, respectively. At the same time, the effects of light treatment, emasculation of the female parent, and flower bud size on the hybridization were investigated. The results showed that under the NSD environment in Sanya, the flowering period and plant height shortened, the flower number and single floral weight decreased, the flower and its constituents became smaller, and the pollen sterility rate increased (P<0.01) as compared with those under LD treatment (P<0.01), Taking the plants with big flower bud under NSD as the female parent, and the plants under LD treatment as the male parent, we made the crosses and pollinated without emasculation, with the hybrid seed-setting rate of 64.36%, which is high enough for breeding purpose. According to the results, we propose the solution for improving the hybrid success rate of soybean in winter nursery.

Key words: Soybean, Winter nursery, Normal short day, Long day treatment, Hybridization technique

[1]周雪松, 刘荣志, 陈冠铭, 李劲松. 南繁: 现状与问题——南繁单位调查报告. 中国农学通报, 2012, 28(24): 161–165



Zhou X S, Liu R Z, Chen G M, Li J S. Breeding in Hainan: present situation and problems—investigation report of breeding in Hainan. Chin Agric Sci Bull, 2012, 28(24): 161–165 (in Chinese with English abstract)



[2]Chen G, Yang J, Lin Y, Wang L, Li J. Study on designing standards system frame work of Hainan national breeding and multiplication. Agric Sci & Technol, 2012, 13: 1437–1442



[3]吉林省农业科学院, 中国大豆育种与栽培. 北京: 农业出版社, 1987. pp 304–306



Jilin Academy of Agricultural Sciences. Soybean Breeding and Cultivation in China. Beijing: Agriculture Press, 1987. pp 304–306 (in Chinese)



[4]吴俊强, 杨兆顺, 楼辰军, 钱芳, 李秀萍. 浅谈海南岛玉米南繁的技术措施. 天津农业科学, 2009, 15(1): 33–34



W J Q, Yang Z S, Lou C J, Qian F, Li X P. Measures on corn breeding in Hainan province. Tianjin Agric Sci, 2009, 15(1): 33–34 (in Chinese with English abstract)



[5]安伟, 南繁管理工作的几点体会与建议. 山西农业科学, 2007, 35(1): 86–88



An W. Suggestions and experiences for crop winter nursery management in Hainan Island. J Shanxi Agric Sci, 2007, 35(1): 86–88 (in Chinese with English abstract)



[6]于伟. 南繁大豆生长特点及丰产栽培技术措施. 大豆科技, 2012, (5): 13–16



Yu W. Growth characteristics and high yield cultivation practices of soybean breeding lines propagated in Hainan province. Soybean Sci Technol, 2012, (5): 13–16 (in Chinese with English abstract)



[7]李磊, 李智, 时和斌. 大豆海南加代的实践与体会. 作物杂志, 2003, (4): 52–53



Li L, Li Z, Shi H B. Practice and experience on soybean southern propagation in Hainan province. Crops, 2003, (4): 52–53 (in Chinese)



[8]Jia H, Wu C, Jiang B, Lu W, Hou W, Sun S, Yan H, Han T, Maturity group classification and maturity locus genotyping of early-maturing soybean varieties from high-latitude cold regions. PLoS One, 2014, 9(4): e94139



[9]吴存祥, 李继存, 沙爱华, 曾海燕, 孙石, 杨光明, 周新安, 常汝镇, 年海, 韩天富. 国家大豆品种区域试验对照品种的生育期组归属. 作物学报, 2012, 38: 1977–1987



Wu C X, Li J C, Sha A H, Zeng H Y, Sun S, Yang G M, Zhou X A, Chang R Z, Nian H, Han T F. Maturity group classification of check varieties in national soybean uniform trials of China. Acta Agron Sin, 2012, 38: 1977−1987 (in Chinese with English abstract)



[10]Fehr W R, Caviness C E. Stages of Soybean Development. Special Report 80, Cooperative Extension Service, Agriculture and Home Economic Experiment Station. Ames, Iowa: Iowa State University, 1977. pp 1–11



[11]Yan J, Wu C, Zhang L, Hu P, Hou W, Zu W, Han T. Long-day effects on the terminal inflorescence development of a photoperiod-sensitive soybean [Glycine max (L.) Merr.] variety. Plant Sci, 2011, 180: 504–510



[12]张勇, 杨兴勇, 董全中, 薛红, 张明明, 刘发, 万培蔚, 宋继玲, 刘长臣. 不去雄蕊杂交技术在海南南繁基地应用的研究. 作物杂志, 2009, (5): 87–88



Zhang Y, Yang X Y, Dong Q Z, Xue H, Zhang M M, Liu F, Wan P W, Song J L, Liu C C. Application of not-removing stamen hybridization technique in soybean in Hainan base. Crops, 2009, (5): 87–88 (in Chinese with English abstract)



[13]许海涛, 王友华, 许波. 夏大豆有性杂交技术与实践. 陕西农业科学, 2006, (4): 153–155



Xu H T, Wang Y H, Xu B. Technique and practice of sexual hybridization of summer soybean. Shaanxi J Agric Sci, 2006, (4): 153–155 (in Chinese)



[14]赵丽梅, 孙寰, 黄梅, 王曙明, 王跃强, 大豆结实率与花粉败育率之间的关系. 大豆科学, 2004, 23: 249–252



Zhao L M, Sun H, Huang M, Wang S M, Wang Y Q. The relationship between seed setting rate and pollen sterility rate for soybean. Soybean Sci, 2004, 23: 249–252 (in Chinese with English abstract)



[15]唐启义, 冯明光. DPS数据处理系统——实验设计、统计分析及模型优化. 北京: 科学出版社, 2006



Tang Q Y, Feng M G. DPS DATA Processing System—Experimental Design, Statistical Analysis and Modeling. Beijing: Science Press, 2006 (in Chinese)



[16]Talukdar A, Shivakumar M. Pollination without emasculation: an efficient method of hybridization in soybean (Glycine max (L.) Merrill). Curr Sci, 2012, 103: 628–630



[17]陈怡. 怎样提高大豆杂交成活率. 黑龙江农业科学, 1985, (3): 40–42



Chen Y. How to increase the survival rate of the artificially-pollinated flowers and young pods in soybean. Heilongjiang Agric Sci, 1985, (3): 40–42 (in Chinese)



[18]Agrawal A P, Ravikumar R L, Salimath P M, Patil S A. Improved method for increasing the efficiency of hybridization in soybean (Glycine max (L.) Merill). Indian J Genet, 2001, 61: 76–77



[19]韩冬伟. 大豆整体去雄杂交技术的研究与实践. 黑龙江农业科学, 2010, (6): 29–31



Han D W. Study and practice of the whole emasculation hybridization technology in soybean. Heilongjiang Agric Sci, 2010, (6): 29–31 (in Chinese with English abstract)



[20]王敏. 影响大豆杂交成活率因素初探. 安徽农学通报, 2009, 15(4): 61–62



Wang M. Preliminary study on factors affecting the survival rate of the artificially-pollinated flowers and young pods in soybean. Anhui Agric Sci Bull, 2009, 15(4): 61–62 (in Chinese)



[21]于伟, 李磊, 李智, 王敏. 大豆的杂交方法与技巧. 作物杂志, 2005, (6): 51–52



Yu W, Li L, Li Z, Wang M. Methods and techniques about soybean hybridization. Crops, 2005, (6): 51–52 (in Chinese)



[22]于文来, 金鑫. 怎样提高大豆杂交成活率. 种子, 1994, 69(1): 55–56



Yu W L, Jin X. How to increase the survival rate of the artificially-pollinated flowers and young pods in soybean. Seed, 1994, 69(1): 55–56 (in Chinese)



[23]张桂茹. 大豆杂交技术. 黑龙江农业科学, 1999, (2): 28–29



Zhang G R. Soybean crossing technique. Heilongjiang Agric Sci, 1999, (2): 28–29 (in Chinese)



[24]Walker A K, Cianzio S R, Bravo J A, Fehr W R. Comparison of emasculation and nonemasculation for hybridization of soybean. Crop Sci, 1979, 19: 285–286



[25]申家恒, 严国忠. 大豆自花受粉时花蕾形态特征的观察. 中国油料, 1981, (3): 16–20



Shen J H, Yan G Z. Morphological observation of flower buds during self-pollination in Glycine max. Oil Crops China, 1981, (3): 16–20 (in Chinese)



[26]申家恒. 大豆受精作用的研究. 植物学报, 1983, 25: 213–221



Shen J H. Studies on fertilization in Glycine max. Acta Bot Sin, 1983, 25: 213–221 (in Chinese with English abstract)



[27]Garner W W, Allard H A. Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plants. J Agric Res, 1920, 18: 553–606



[28]Garner W W, Allard H A. Further studies in photoperiodism, the response of the plant relative length of day and night. J Agric Res, 1923, 23: 871–920



[29]盖钧镒, 赵团结, 崔章林, 邱家训. 中国1923–1995年育成的651个大豆品种的遗传基础. 中国农业科技导报, 1999, (1): 26–30



Gai J Y, Zhao T J, Cui Z L, Qiu J X. The Genetic base for 651 soybean cultivars released during 1923–1995 in China. Rev Chin Agric Sci Technol, 1999, (1): 26–30 (in Chinese with English abstract)



[30]盖钧镒, 赵团结, 崔章林, 邱家驯. 中国大豆育成品种中不同地理来源种质的遗传贡献. 中国农业科学, 1998, 31(5): 35–43



Gai J Y, Zhao T J, Cui Z L, Qiu J X. Nuclear and cytoplasmic contributions of germplasm from distinct areas to the soybean cultivars released during 1923–1995 in China. Sci Agric Sin, 1998, 31(5): 35–43 (in Chinese with English abstract)



[31]熊冬金, 赵团结, 盖钧镒. 中国大豆育成品种亲本分析. 中国农业科学, 2008, 41: 2589–2599



Xiong D J, Zhang T J, Gai J Y. Parental analysis of soybean cultivars released in China. Sci Agric Sin, 2008, 41: 2589–2598 (in Chinese with English abstract)



[32]王彩洁, 孙石, 金素娟, 李伟, 吴存祥, 侯文胜, 韩天富. 中国大豆主产区不同年代大面积种植品种的遗传多样性分析. 作物学报, 2013, 39: 1917–1926



Wang C J, Sun S, Jing S J, Li W, Wu C X, Hou W S, Han T F. Genetic diversity analysis of widely-planted soybean varieties from different decades and major production regions in China. Acta Agron Sin, 2013, 39: 1917–1926 (in Chinese with English abstract)



[33]王彩洁, 孙石, 吴宝美, 常汝镇, 韩天富. 20世纪40年代以来中国大面积种植大豆品种的系谱分析. 中国油料作物学报, 2013, 35: 246–252



Wang C J, Sun S, Wu B M, Chang R Z, Han T F. Pedigree analysis of widely-planting soybean varieties in China since 1940s. Chin J Oil Crop Sci, 2013, 35: 246–252 (in Chinese with English abstract)

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