Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (03): 386-393.doi: 10.3724/SP.J.1006.2015.00386

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Bacterial Blight Resistance Gene in Yunnan Wild Rice

LI Ding-Qin,CHEN Ling,LI Wei-Jiao,KE Xue,YU Teng-Qiong,LI E-Xian,HUANG Xing-Qi,CHENG Zai-Quan*   

  1. 1Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences / Yunnan Provincial Key Laboratory of Agricultural Biotechnology / Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650223, China
  • Received:2014-09-23 Revised:2014-12-19 Online:2015-03-12 Published:2015-01-12
  • Contact: 程在全,E-mail: czquan-99@163.com E-mail:lidingqin37@aliyun.com

Abstract:

Bacterial blight (BB) is one of the most destructive diseases in rice production. The most efficient and economical way of controlling the disease is developing disease resistance varieties. Owing to the limited number of resistance genes from cultivated rice and the narrow spectrum of some bacterial blight resistance genes, exploring new resistance genes from wild rice will be conducive to development of new resistance varieties with wider spectrum and higher resistance. Yunnan is rich in resources of wild rice. However, till now there are few reports concerning whether they contain seven cloned BB resistance genes. In this study, seven cloned BB genes were detected in Yunnan wild rice by resistance identification and PCR analysis. The results showed that Yunnan wild rice had different levels of resistance to BB strains Y8 and PXO99, and O. granulata was immune to Y8 and PXO99. The resistance genes xa5, xa13 and Xa21 were not found in all three wild rice materials. Xa1 was detected in O. rufipogon type from Jinghong. Xa3/Xa26 was identified in O. rufipogon types from Yuanjiang and Jinghong, respectively. Xa3/Xa26 was alsoidentified in O. officinalis, Xa23 was found in O. rufipogon type from Yuanjiang, Xa27 in O. rufipogon type from Jinghong, and Xa27 in O. granulata. The results provided a theoretical reference for further exploring and cloning new BB resistance genes in Yunnan wild rice to improve the disease resistance of cultivated rice.

Key words: Wild rice, Bacterial blight, Resistance gene, PCR identification

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