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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (09): 1411-1416.doi: 10.3724/SP.J.1006.2016.01411

• RESEARCH NOTES • Previous Articles    

Identification of Resistance to Brown Rust and Molecular Detection of Bru1 Gene in 101 Main Sugarcane Breeding Parents in China

LI Wen-Feng,WANG Xiao-Fan,HUANG Ying-Fun*,ZHANG Rong-Yue,SHAN Hong-Li,LUO Zhi-Ming,YIN Jiong   

  1. Sugarcane Research Institute, Yunnan Province Academy of Agricultural Sciences,, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
  • Received:2016-03-17 Revised:2016-06-20 Online:2016-09-12 Published:2016-06-27
  • Contact: 黄应昆, E-mail: huangyk64@163.com, Tel: 0873-7227017 E-mail:ynlwf@163.com
  • Supported by:

    This study was supported by China Agriculture Research System (CARS-20-2-2) and the Agriculture Research System of Yunnan Province.

Abstract:

Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Bru1 is a major brown rust resistance gene with a broad-spectrum resistance against various brown rust pathogens originating from different countries. In order to assess the resistance level of main sugarcane breeding parents to Puccinia melanocephala, and determine the distribution of the Bru1 gene in these breeding parents in China, 101 main sugarcane breeding parents collected from the Chinese National Nursery of Sugarcane Germplasm Resources were evaluated and identified for their brown rust resistance at the seedling stage and for the presence of the brown rust resistance gene Bru1. Bru1 was detected to present in 48 (47.5%) resistant clones out of the 101 tested parental lines, indicating that brown rust resistance in main sugarcane breeding parents in China is primarily controlled by Bru1. The absence of Bru1 in the other 29 resistant and 24 susceptible parental lines suggests that they may carry brown rust resistance-associated genes other than Bru1. These results may facilitate future efforts to breed varieties with improved resistance to brown rust and may provide resistant parental lines for selecting and planting elite resistant varieties to effectively control brown rust disease in sugarcane.

Key words: Sugarcane, Main breeding parents, Brown rust resistance gene Bru1, Molecular detection.

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