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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (03): 315-323.doi: 10.3724/SP.J.1006.2017.00315

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Influence of OsWR2-RNAi on Rice Cuticle Biosynthesis and Drought Resistance

WANG Sha1,HE Yong1,LUO Guang-Yu1,YAO Min1,ZHANG Xu1,CHEN Xin-Bo1,2,ZHOU Xiao-Yun1,2,*   

  1. 1 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; 2 Crop Gene Engineering Key Laboratory of Hunan Province, Changsha 410128, China
  • Received:2016-07-20 Revised:2016-11-02 Online:2017-03-12 Published:2016-11-11
  • Contact: 周小云, E-mail: xyzhou71@hotmail.com E-mail:236816019@qq.com
  • Supported by:

    This study was supported by the Key Research Projects of Hunan (2015JC3102), the College Students Innovation Project of Hunan Agricultural University (XCX1504), and the National Natural Science Foundation of China (31300216).

Abstract:

Plant cuticle plays an important role on decreasing plant non-stomatal water loss under drought stress. Overexpression of wax related transcription factor gene OsWR2 in rice can increase total cuticular wax and cutin amounts, affect cuticular wax and cutin monomer composition, and alter cuticular wax crystallization patterns and cuticle membrane ultrastructure, implying important roles of OsWR2 in rice cuticle biosynthesis and non-stomatal water loss regulation. In this report, we constructed OsWR2 RNA inhibition vector (OsWR2-RNAi), and transformed it into rice callus to obtain OsWR2 knockout rice mutants using Agrobacterium-mediated transforming method. OsWR2-RNAi transgenic rice exhibited reduced total cuticular wax amounts by 14.8% mainly due to the decrease of aldehydes, alkanes and alcohols, and lessened full cutin monomer level by 36.2% due primarily to the decrease of C16:0, C18:1 ω-OH and di-OH fatty acid components. OsWR2-RNAi transgenic rice also showed enriched leaf chlorophyll leaching, water loss rates and MDA contents, whereas reduced tolerance to water deficit . All these physiologic and biochemical index to support the previous hypothesis ofOsWR2 acting as a transcriptional regulator of both cuticle biosynthetic pathways and non-stomatal water loss, providing evidence of OsWR2 exerting direct influence over rice dehydration, and a potential application resource in genetic improvement of crop drought tolerance.

Key words: OsWR2, RNAi, Plant cuticle, Drought stress, Non-stomatal water loss

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