水稻咪草烟抗性的遗传分析及其紧密连锁分子标记的筛选与应用

doi:10.3724/SP.J.1006.2018.00716

Abstract

Abstract:

Cultivation and extension of herbicide-resistant rice can increase the efficiency of weed control and obtain great economic benefits. Here, Jinjing 818 was identified as an imazethapyr-resistant line, F₂ population from the cross between Jinjing 818 and the conventional japonica rice Suken 118 was used for genetic analysis and gene mapping of the imazethapyr resistance in rice. The imazethapyr resistance in Jinjing 818 was controlled by a single dominant nuclear gene located between SSR markers RM7413 and RM7426 on the chromosome 2. Through gene-predicting and sequencing in this region, we found that the target gene of imazethapyr, acetolactate synthase gene (ALS), had a nucleotide mutation (G mutated into A) in its coding region, leading to the mutation of serine into asparagine. It could be preliminarily deduced that ALS is the candidate gene for imazethapyr resistance. The physical distances between RM7413, RM7426 and ALS were 165 kb, 1612 kb, respectively. Jinjing 818 and Nanjing 9108 were used as parents to detect the application potential of RM7413 in molecular marker assisted selection breeding (MAS). On the basis of selecting phenotype and RM7413 in the hybrid and its selfing generation, F₇ population showed stable imazethapyr resistance, indicating that the great application potential of RM7413 in MAS and breeding for imazethapyr resistant rice. Our results will lay a foundation for breeding herbicide-resistant japonica rice by MAS.

Key words: Oryza sativa subsp. Keng, Molecular marker assisted selection, RM7413, Jinjing 818, Imazethapyr

Yun-Yan FEI, Jie YANG, Fang-Jun FAN, Fang-Quan WANG, Wen-Qi LI, Jun WANG, Jin-Yan ZHU, Wei-Gong ZHONG. Genetic Analysis of Imazethapyr Resistance in Rice and the Closely Linked Marker Selection and Application[J].Acta Agronomica Sinica, 2018, 44(05): 716-722.

Figures/Tables 7

Table 1

Ninety-one commercial japonica rice varieties in the middle and lower reaches of Yangtze River"

生育特性 Maturing type	品种 Cultivar
中熟中粳 Medium-maturing medium japonica rice	连粳4号, 连粳7号, 连粳9号, 宁粳4号, 徐稻3号, 镇稻88, 镇稻99, 连粳10号, 连粳11, 华粳5号, 华粳6号, 淮稻8号, 淮稻11, 淮优粳2号, 盐稻11, 盐粳11, 武运粳21, 武运粳27, 华瑞稻1号, 淮糯11, 淮粳096, 扬中稻1号, 扬辐粳1号, 扬粳4308, 泗稻12 Lianjing 4, Lianjing 7, Lianjing 9, Ningjing 4, Xudao 3, Zhendao 88, Zhendao 99, Lianjing 10, Lianjing 11, Huajing 5, Huajing 6, Huaidao 8, Huaidao 11, Huaiyoujing 2, Yandao 11, Yanjing 11, Wuyunjing 21, Wuyunjing 27, Huaruidao 1, Huainuo 11, Huaijing 096, Yangzhongjing 1, Yangfujing 1, Yangjing 4308, Sidao 12
迟熟中粳 Late-maturing medium japonica rice	淮稻5号, 盐稻8号, 盐稻9号, 南粳49, 南粳40, 南粳41, 南粳45, 扬育粳2号, 扬辐粳8号, 苏沪香粳, 武陵粳1号, 武育粳3号, 武运粳24, 华粳3号, 华粳4号, 华粳7号, 淮稻7号, 淮稻9号, 淮稻10号, 淮稻13, 盐粳9号, 盐粳10号, 宁粳5号, 武运粳4号, 武运粳11 Huaidao 5, Yandao 8, Yandao 9, Nanjing 49, Nanjing 40, Nanjing 41, Nanjing 45, Yangyujing 2, Yangfujing 8, Suluxiangjing, Wulingjing 1, Wuyunjing 3, Wuyunjing 24, Huajing 3, Huajing 4, Huajing 7, Huaidao 7, Huaidao 9, Huaidao 10, Huaidao 13, Yanjing 9, Yanjing 10, Ningjing 5, Wuyunjing 4, Wuyunjing 11
早熟晚粳 Early-maturing late japonica rice	宁粳1号, 南粳5055, 南粳44, 武香粳14, 南粳47, 武运粳7号, 镇稻18, 淮香稻15, 宁粳3号, 南粳42, 通粳981, 武粳13, 武粳15, 武香粳9号, 武运粳19, 武运粳23, 武运粳29, 常农粳4号, 常农粳5号, 常农粳7号, 镇稻7号, 镇稻10号, 镇稻15, 镇稻16, 镇糯19, 宁粳2号, 常粳144, 苏粳5号, 镇稻1号, 镇稻9424 Ningjing 1, Nanjing 5055, Nanjing 44, Wuxiangjing 14, Nanjing 47, Wuyunjing 7, Zhendao 18, Huaixiangdao 15, Ningjing 3, Nanjing 42, Tongjing 981, Wujing 13, Wujing 15, Wuxiangjing 9, Wuyunjing 19, Wuyunjing 23, Wuyunjing 29, Changnongjing 4, Changnongjing 5, Changnongjing 7, Zhendao 7, Zhendao 10, Zhendao 15, Zhendao 16, Zhennuo 19, Ningjing 2, Changjing 144, Sujing 5, Zhendao 1, Zhendao 9424
中熟晚粳 Medium-maturing late japonica rice	南粳46, 扬粳4227, 武育粳18, 常农粳3号, 常农粳6号, 苏粳8号, 苏香粳2号, 镇稻12, 镇稻13, 镇稻17, 苏粳9号 Nanjing 46, Yangjing 4227, Wuyujing 18, Changnongjing 3, Changnongjing 6, Sujing 8, Suxiangjing 2, Zhendao 12, Zhendao 13, Zhendao 17, Sujing 9

Table 1

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References 24

[1]	Camargo E R, Senseman S A, Mccauley G N, Guice J B.Rice (Oryza sativa L.) response and weed control from tank-mix applications of saflufenacil and imazethapyr. Crop Prot, 2012, 31: 94-98 doi: 10.1016/j.cropro.2011.09.015
[2]	Norsworthy J K, Scott R C, Bangarwa S K, Griffith G M, Wilson M J, Mccelland M.Weed management in a furrow-irrigated imidazolinone-resistant hybrid rice production system.Weed Technol, 2011, 25: 25-29 doi: 10.1614/WT-D-10-00088.1
[3]	Kumar V, Bellinder R R, Gupta R K, Malik R K, Brainard D C.Role of herbicide-resistant rice in promoting resource conservation technologies in rice-wheat cropping systems of India: a review.Crop Prot, 2008, 27: 290-301 doi: 10.1016/j.cropro.2007.05.016
[4]	苏少泉. 抗除草剂水稻品种的创制与发展. 农药研究与应用, 2010, (5): 6-8
	Su S Q.The herbicide-resistant rice varieties expected and development.Agrochem Res Appl, 2010, (5): 6-8 (in Chinese with English abstract)
[5]	Tan S, Dahmer M L, Singh B K, Shaner D L.Imidazolinone- tolerant crops: history, current status and future. Pest Manag Sci, 2010, 61: 246-257 doi: 10.1002/ps.993 pmid: 15627242
[6]	Kawai K, Kaku K, Izawa N, Shimizu T, Fukuda A, Tanaka Y.A novel mutant acetolactate synthase gene from rice cells, which confers resistance to ALS-inhibiting herbicides.J Pestic Sci, 2007, 32: 89-98 doi: 10.1584/jpestics.G06-40
[7]	Masson J A, Webster E P.Use of imazethapyr in water-seeded imidazolinone-tolerant rice (Oryza sativa L.). Weed Technol, 2001, 15: 103-106 doi: 10.1614/0890-037X(2001)015[0103:UOIIWS]2.0.CO;2
[8]	肖国樱, 陈芬, 孟秋成, 周浩, 李锦江, 于江辉, 邓力华, 翁绿水. 我国转基因抗除草剂水稻的生态风险与控制. 农业生物技术学报, 2015, 23: 1-11 doi: 10.3969/j.issn.1674-7968.2015.01.001
	Xiao G Y, Chen F, Meng Q C, Zhou H, Li J J, Yu J H, Deng L H, Weng L S.Ecological risk and management of herbicide-resistant transgenic rice (Oryza sativa L.) in China. J Agric Biotechnol, 2015, 23: 1-11 (in Chinese with English abstract) doi: 10.3969/j.issn.1674-7968.2015.01.001
[9]	李余生, 陈涛, 虞秋成, 黄宝才, 王才林. 水稻抗条纹叶枯病基因Stv-bi连锁分子标记的鉴定及利用. 江苏农业学报, 2009, 25: 459-463
	Li Y S, Chen T, Yu Q C, Huang B C, Wang C L.Identification and application of SSR markers linked to resistant gene Stv-bi for Rice strip virus. Jiangsu J Agric Sci, 2009, 25: 459-463 (in Chinese with English abstract)
[10]	胡茂龙, 龙卫华, 高建芹, 付三雄, 陈锋, 周晓婴, 彭琦, 张维, 浦惠明, 戚存扣. 油菜抗咪唑啉酮类除草剂基因BnALS1R等位基因特异PCR标记的开发与应用. 作物学报, 2013, 39: 1711-1719
	Hu M L, Long W H, Gao J Q, Fu S X, Chen F, Zhou X Y, Peng Q, Zhang W, Pu H M, Qi C K.Development and application of allele-specific PCR markers for imidazolinone-resistant gene BnALS1R in Brassica napus. Acta Agron Sin, 2013, 39: 1711-1719 (in Chinese with English abstract)
[11]	李志江, 李延东, 马金丰, 李祥羽, 赵丽娟, 王绍滨. 谷子抗“拿捕净”基因的SSR标记. 黑龙江农业科学, 2013, (7): 5-7 doi: 10.3969/j.issn.1002-2767.2013.07.002
	Li Z J, Li Y D, Ma J F, Li X Y, Zhao L J, Wang S B.SSR marker for “Napujing”-resistant gene in Setaria italica. Heilongjiang Agric Sci, 2013, (7): 5-7 (in Chinese) doi: 10.3969/j.issn.1002-2767.2013.07.002
[12]	Bulos M, Sala C A, Altieri E, Ramos M L.Marker assisted selection for herbicide resistance in sunflower.Helia, 2013, 36(59): 1-16 doi: 10.2298/hel1359001b
[13]	Mithila J, McLean M D, Chen S, Christopher H J. Development of near-isogenic lines and identification of markers linked to auxinic herbicide resistance in wild mustard (Sinapis arvensis L.). Pest Manag Sci, 2012, 68: 548-556
[14]	Michelmore R W, Paran I, Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proc Natl Acad Sci USA, 1991, 88: 9828-9832 doi: 10.1073/pnas.88.21.9828
[15]	Murray M G, Thompson W F.Rapid isolation of high molecular weight plant DNA.Nucl Acids Res, 1980, 8: 4321-4325 doi: 10.1093/nar/8.19.4321
[16]	Luo Z, Yang Z, Zhong B, Li Y, Xie R, Zhao F, Ling Y, He G.Genetic analysis and fine mapping of a dynamic rolled leaf gene, RL10(t), in rice(Oryza sativa L.). Genome, 2007, 50: 811-817 doi: 10.1139/g07-064 pmid: 17893721
[17]	Givens W A, Shaw D R, Newman M E, Weller S C, Young B G, Wilson R G, Owen M D, Jordan D L.Benchmark study on glyphosate-resistant cropping systems in the United States.Pest Manag Sci, 2011, 67: 758-770 doi: 10.1002/ps.v67.7
[18]	王军, 宫丹妮, 范方军, 朱金燕, 李文奇, 王芳权, 仲维功, 杨杰. 不同类型江苏粳稻主推品种的遗传多样性分析. 中国农学通报, 2016, 32(36): 24-30
	Wang J, Gong D N, Fan F J, Zhu J Y, Li W Q, Wang F Q, Zhong W G, Yang J.Genetic diversity analysis of major japonica cultivars of different ecotypes in Jiangsu. Chin Agric Bull, 2016, 32(36): 24-30 (in Chinese with English abstract)
[19]	周振玲, 王宝祥, 樊继伟, 卢百关, 赵志刚, 江玲, 秦德荣, 万建民, 徐大勇. 江淮稻区不同生态型粳稻品种的籼粳分化度和遗传多样性. 中国水稻科学, 2012, 26: 431-437
	Zhou Z L, Wang B X, Fan J W, Lu B G, Zhao Z G, Jiang L, Qin D R, Wan J M, Xu D Y.Indica-japonica differentiation degree and genetic diversity of japonica cultivars belongs to different ecotypes from the Yangtza-Huaihe region. Chin J Rice Sci, 2012, 26: 431-437 (in Chinese with English abstract)
[20]	Wang C H, Zheng X M, Xu Q, Yuan X P, Huang L, Zhou H F, Wei X H, Ge S.Genetic diversity and classification of Oryza sativa with emphasis on Chinese rice germplasm. Heredity, 2014, 112: 489-496 doi: 10.1038/hdy.2013.130 pmid: 24326293
[21]	程芳艳, 李春光, 刘永巍, 孙翊轩, 王继亮, 孟昭河, 徐正进. 寒地部分粳稻的遗传多样性及遗传结构分析. 沈阳农业大学学报, 2014, 45: 649-654 doi: 10.3969/j.issn.1000-1700.2014.06.002
	Cheng F Y, Li C G, Liu Y W, Sun Y X, Wang J L, Meng Z H, Xu Z J.Analysis of genetic diversity and genetic structure about rice materials from cold region.J Shenyang Agric Univ, 2014, 45: 649-654 (in Chinese with English abstract) doi: 10.3969/j.issn.1000-1700.2014.06.002
[22]	甘晓燕, 李苗, 关雅静, 陈晓军, 宋玉霞. 宁夏89份粳稻种质遗传多样性的SSR分析. 西北植物学报, 2009, 29: 1772-1778 doi: 10.3321/j.issn:1000-4025.2009.09.008
	Gan X Y, Li M, Guan Y J, Chen X J, Song Y X.Genetic diversity of 89 japonica rice varieties in Ningxia province by using SSR. Acta Bot Boreal-Occident Sin, 2009, 29: 1772-1778 (in Chinese with English abstract) doi: 10.3321/j.issn:1000-4025.2009.09.008
[23]	Becerra V, Paredes M, Ferreira M E, Gutiérrez E, Díaz L M.Assessment of the genetic diversity and population structure in temperate japonica rice germplasm used in breeding in Chile, with SSR markers. Chil J Agric Res, 2017, 77: 15-26
[24]	Beckie H J, Hall L M.Genetically-modified herbicide-resistant (GMHR) crops a two-edged sword? An Americas perspective on development and effect on weed management.Crop Prot, 2014, 66: 40-45 doi: 10.1016/j.cropro.2014.08.014

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Genetic Analysis of Imazethapyr Resistance in Rice and the Closely Linked Marker Selection and Application

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