割手密萜烯合成酶(TPS)基因家族分析及其在生物胁迫下的表达分析

doi:10.3724/SP.J.1006.2022.14237

Abstract

Abstract:

Terpenoids produced by the action of terpene synthase (TPS) enzymes play important roles in plant biotic and abiotic stress. Saccharum spontaneum, an important parent material of modern sugarcane cultivars, contains a large number of stress resistance genes. To investigate the characteristics and functions of the TPS gene family in S. spontaneum, 39 SsTPS genes were identified in S. spontaneum genome that encoded proteins with two conserved domains (PF01397 and PF03936) by using an HMMER search. The SsTPS proteins were divided into TPS-a, b, e/f, and g subfamilies. The SsTPS gene family had mainly expanded through segmental duplications, and a total of 12 SsTPS genes involved in segmental duplication events. In addition, qRT-PCR showed that the expression patterns of some SsTPS genes differed in S. spontaneum between Spodoptera frugiperda-stressed and Xanthomonas albilineans-infected plants, whereas the relative expression levels of seven SsTPS genes were strongly up-regulated. Notably, SsTPS15 were up-regulated in response to Spodoptera frugiperda-stressed but were down-regulated by X. albilineans infection, while SsTPS26, SsTPS37, and SsTPS39 had the opposite results. These results will be of great significance to further understanding the biological roles of terpene synthases and to develop resistant breeding in S. spontaneum.

Key words: Saccharum spontaneum, terpene synthase gene, biotic stresses, expression analysis, resistance breeding

LIN Huan-Tai, ZHANG Tian-Jie, SHI Meng-Ting, GUO Yan-Fang, GAO San-Ji, WANG Jin-Da. Genome-wide analysis of terpene synthase (TPS) gene family and its expression under biological stress in Saccharum spontaneum[J].Acta Agronomica Sinica, 2022, 48(12): 3029-3044.

Figures/Tables 10

Table 1

Table 2

Basin information and physiochemical properties of SsTPS genes family in S. spontaneum"

基因名称 Gene name	基因编号 Gene ID	氨基酸数 Number of amino acids	等电点 pI	分子量 Molecular weight (kD)	负电荷残基 Asp+Glu	正电荷残基Arg+Lys	不稳定系数Coefficient of instability	平均疏水性 GRAVY	脂肪系数 AI	α-螺旋 α-helix(%)	延伸链 Extended chain (%)	无规则卷曲 Random curl (%)
基因名称 Gene name	基因编号 Gene ID	氨基酸数 Number of amino acids	等电点 pI	分子量 Molecular weight (kD)	负电荷残基 Asp+Glu	正电荷残基Arg+Lys	不稳定系数Coefficient of instability	平均疏水性 GRAVY	脂肪系数 AI	α-螺旋 α-helix(%)	延伸链 Extended chain (%)	无规则卷曲 Random curl (%)	SsTPS1	Sspon.01G0009730-1A	514	5.90	59,392.66	72	62	36.28	-0.306	88.29	57.39	11.67	30.93
SsTPS2	Sspon.01G0010010-1A	496	5.29	57,214.41	78	57	37.65	-0.288	94.92	54.03	14.72	31.25
SsTPS3	Sspon.01G0044880-1B	497	5.75	56,761.45	69	54	40.04	-0.312	83.84	49.90	17.51	32.60
SsTPS4	Sspon.04G0011840-1A	735	9.77	81,962.81	74	104	60.66	-0.460	75.17	43.67	8.03	48.30
SsTPS5	Sspon.04G0011840-2B	353	5.78	41,253.51	51	43	47.03	-0.333	87.00	64.31	3.68	32.01
SsTPS6	Sspon.04G0011840-3C	501	6.43	57,711.12	62	58	45.18	-0.311	87.96	55.09	6.99	37.92
SsTPS7	Sspon.05G0015000-1A	581	5.53	66,604.19	78	61	39.07	-0.216	91.02	51.29	12.22	36.49
SsTPS8	Sspon.06G0010490-1P	528	5.33	61,308.56	76	59	39.80	-0.173	98.26	52.65	12.12	35.23
SsTPS9	Sspon.06G0010500-1A	376	5.10	43,465.54	59	41	52.71	-0.328	87.95	52.39	11.17	36.44
SsTPS10	Sspon.06G0011520-1A	433	5.84	49,561.84	62	51	40.69	-0.242	83.44	55.43	9.70	34.87
SsTPS11	Sspon.06G0011680-1A	468	5.32	53,911.93	71	58	49.94	-0.247	97.67	60.47	7.69	31.84
SsTPS12	Sspon.06G0010490-1A	538	5.34	62,470.81	78	61	40.28	-0.201	96.43	50.74	12.64	36.62
SsTPS13	Sspon.06G0021690-1B	325	5.52	37,701.75	51	39	46.74	-0.380	89.14	55.38	12.62	32.00
SsTPS14	Sspon.06G0023410-1B	397	5.47	46,236.32	64	52	38.72	-0.254	96.93	50.13	12.85	37.03
SsTPS15	Sspon.06G0010490-2B	538	5.34	62,468.83	78	61	40.74	-0.192	97.16	50.93	12.64	36.43
SsTPS16	Sspon.06G0011680-2B	607	5.96	70,631.16	81	70	49.39	-0.222	94.23	50.74	13.67	35.58
SsTPS17	Sspon.06G0024130-1B	606	5.97	70,317.44	86	74	53.41	-0.363	83.83	55.61	13.53	30.86
SsTPS18	Sspon.06G0015570-2B	533	5.29	61,439.38	76	57	45.23	-0.220	83.49	50.66	14.82	34.52
SsTPS19	Sspon.06G0023520-2C	491	5.08	57,333.63	76	54	49.64	-0.302	84.83	46.84	13.65	39.51
SsTPS20	Sspon.06G0011660-2C	482	5.04	55,885.74	75	53	48.91	-0.274	86.72	47.51	18.67	33.82
SsTPS21	Sspon.06G0011680-1P	414	5.47	47,542.37	62	48	48.39	-0.301	93.16	53.86	10.63	35.51
SsTPS22	Sspon.06G0030650-1C	358	9.40	41,189.25	38	51	38.63	-0.391	79.55	46.37	13.69	39.94
SsTPS23	Sspon.06G0011680-3C	468	5.15	53,761.70	72	55	51.78	-0.219	98.93	58.55	9.83	31.62
SsTPS24	Sspon.06G0034360-1D	317	4.93	36,898.43	50	32	39.19	-0.134	91.29	48.26	17.98	33.75
SsTPS25	Sspon.06G0011680-2P	333	8.65	38,083.58	38	42	35.22	-0.328	83.75	38.44	21.32	40.24
SsTPS26	Sspon.06G0024130-2D	545	5.56	63,780.15	81	68	48.69	-0.286	90.11	53.39	13.58	33.03
SsTPS27	Sspon.06G0024130-1P	497	5.71	57,610.39	75	65	43.03	-0.181	93.00	51.71	17.30	30.99
SsTPS28	Sspon.06G0011680-4D	328	8.47	37,598.00	37	40	33.44	-0.324	82.65	42.38	17.38	40.24
SsTPS29	Sspon.07G0027750-1B	509	5.55	59,706.37	75	59	55.08	-0.414	90.84	52.65	9.23	38.11
SsTPS30	Sspon.08G0029890-1D	629	5.83	71,361.82	80	67	49.40	-0.160	86.38	46.26	13.67	40.06
SsTPS31	Sspon.01G0039100-1B	348	5.62	39,581.24	49	39	48.50	-0.288	84.74	47.14	12.93	39.66
SsTPS32	Sspon.01G0039100-2C	550	8.25	62,827.75	67	70	54.38	-0.376	82.91	50.36	9.45	40.18
SsTPS33	Sspon.04G0000830-1A	388	5.42	44,015.32	47	38	54.09	-0.088	93.84	55.41	7.47	37.11
SsTPS34	Sspon.05G0030980-1C	545	6.04	62,692.00	73	67	49.04	-0.309	84.02	41.47	12.29	46.24
SsTPS35	Sspon.05G0002480-3D	783	6.03	87,936.45	90	18	46.73	-0.168	89.13	47.51	11.11	41.38
SsTPS36	Sspon.04G0020000-1A	550	5.33	62,580.79	74	53	52.94	-0.116	92.62	49.27	16.55	34.18
SsTPS37	Sspon.04G0019960-2C	515	5.28	59,084.06	71	52	47.41	-0.090	96.37	53.59	14.17	32.23
SsTPS38	Sspon.04G0020000-4P	563	6.13	63,167.56	68	58	50.45	-0.145	89.68	52.75	11.90	35.35
SsTPS39	Sspon.04G0020000-3D	552	5.25	63,021.29	78	56	51.02	-0.123	95.27	54.53	11.78	33.70

Table 2

Fig. 1

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Fig. 8

References 60

[1]	Tholl D. Biosynthesis and biological functions of terpenoids in plants. Adv Biochem Eng Biotechnol, 2015, 148: 63-106.
[2]	韩娟娟, 李喜旺, 刘丰静, 辛肇军, 张瑾, 张新, 孙晓玲. 茶丽纹象甲对茶树品种的取食选择及其诱导的4种萜烯类化合物. 茶叶科学, 2017, 37: 220-227.
	Han J J, Li X W, Liu F J, Xin Z J, Zhang J, Zhang X, Sun X L. Feeding selection of tea cultivars by the tea weevil and the four induced terpenoids. J Tea Sci, 2017, 37: 220-227. (in Chinese with English abstract)
[3]	Hunsigi G, Yekkeli N R, Perumal L, Thippannavar M B. Antibiosis in sugarcane genotypes against woolly aphid Ceratavacuna lanigera Zehntner. Curr Sci India, 2006, 90: 771-772.
[4]	赵善欢, 曹毅, 彭中健, 黄家总. 应用天然植物产品川楝素防治菜青虫试验. 植物保护学报, 1985, 12: 125-132.
	Zhao S H, Cao Y, Peng Z J, Huang J Z. Experiments on the control of Pieris rapae with Toosendanin. J Plant Prot, 1985, 12: 125-132. (in Chinese)
[5]	Irmisch S, Jiang Y, Chen F, Gershenzon J, Köllner T G. Terpene synthases and their contribution to herbivore-induced volatile emission in western balsam poplar (Populus trichocarpa). BMC Plant Biol, 2014, 14: 270. doi: 10.1186/s12870-014-0270-y pmid: 25303804
[6]	De Moraes C M, Mescher M C, Tumlinson J H. Caterpillar- induced nocturnal plant volatiles repel conspecific females. Nature, 2001, 410: 577-580. doi: 10.1038/35069058
[7]	Cheniclet C. Effects of wounding and fungus inoculation on terpene producing systems of maritime pine. J Exp Bot, 1987, 38: 1557-1572. doi: 10.1093/jxb/38.9.1557
[8]	孟雪, 王志英, 吕慧. 绿萝和常春藤主要挥发性成分及其对5种真菌的抑制活性. 园艺学报. 2010, 37: 971-976.
	Meng X, Wang Z Y, Lyu H. The volatile constituents analysis of Scindapsus aureum and Hedera nepalensis var. sinensis and their inhibition against five fungi. Acta Hortic Sin, 2010, 37: 971-976. (in Chinese with English abstract)
[9]	Cui J, Liang J. Research progress of antibacterial effects of citrus peel essential oils. Sci Technol Cereals, 2018, 26: 35-39.
[10]	Lu K, Li X, Zhou J, Xie X, Qi S, Zhou Q. Influence of the herbivore-induced rice volatiles on fungal disease. Chin Sci Bull, 2010, 55: 47-52.
[11]	Liu F, Zuo Z J, Xu G P, Wu X B, Zheng J, Gao R F, Zhang R M, Gao Y. Physiological responses to drought stress and the emission of induced volatile organic compounds in Rosmarinus officinalis. J Plant Ecol, 2013, 37: 454-463.
[12]	Blanch J S, Peñuelas J, Llusià J. Sensitivity of terpene emissions to drought and fertilization in terpene-storing Pinus halepensis and non-storing Quercus ilex. Physiol Plant, 2007, 131: 211-225.
[13]	Kainulainen P, Oksanen J, Palomäki V, Holopainen J K, Holopainen T. Effect of drought and waterlogging stress on needle monoterpenes of Picea abies. Can J Bot, 1992, 70: 1613-1616. doi: 10.1139/b92-203
[14]	Finn R D, Coggill P, Eberhardt R Y, Eddy S R, Mistry J, Mitchell A L, Potter S C, Punta M, Quewshi M, Sangrador-Vegas A, Salazar G A, John Tate S, Bereman A. The Pfam protein families database: towards a more sustainable future. Nucleic Acids Res, 2015, 44: D279-D285.
[15]	Starks C M, Back K, Chappell J, Novel J P. Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase. Science, 1997, 277: 1815-1820. pmid: 9295271
[16]	Falara V, Akhtar T A, Nguyen T H, Spyropoulou E A, Bleeker P M, Schauvinhold I, Matsuba Y, Bonini M E, Schilmiller A L, Last R L. The tomato terpene synthase gene family. Plant Physiol, 2011, 157: 770-789. doi: 10.1104/pp.111.179648 pmid: 21813655
[17]	Jiang S Y, Jin J, Sarojam R, Ramachandran S. A comprehensive survey on the terpene synthase gene family provides new insight into its evolutionary patterns. Genome Biol Evol, 2019, 11: 2078-2098. doi: 10.1093/gbe/evz142
[18]	Chen F, Tholl D, Bohlmann J R, Pichersky E. The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom. Plant J, 2011, 66: 212-229. doi: 10.1111/j.1365-313X.2011.04520.x
[19]	Nieuwenhuizen N J, Green S A, Chen X, Bailleul E J D, Matich A J, Wang M Y, Atkinson R G. Functional genomics reveals that a compact terpene synthase gene family can account for terpene volatile production in apple. Plant Physiol, 2013, 161: 787-804. doi: 10.1104/pp.112.208249 pmid: 23256150
[20]	Tuskan G A, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science, 2006, 313: 1596-1604. pmid: 16973872
[21]	Li G, Koellner T G, Yin Y, Jiang Y, Chen H, Xu Y, Gershenzon J, Pichersky E, Chen F. Nonseed plant Selaginella moellendorfii has both seed plant and microbial types of terpene synthases. Proc Natl Acad Sci USA, 2012, 109: 14711-14715. doi: 10.1073/pnas.1204300109
[22]	Bohlmann J, Meyer-Gauen G, Croteau R. Plant terpenoid synthases: molecular biology and phylogenetic analysis. Proc Natl Acad Sci USA, 1998, 95: 4126-4133. doi: 10.1073/pnas.95.8.4126
[23]	Degenhardt J, Kllner T G, Gershenzon J. Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants. Phytochemistry, 2009, 70: 1621-1637. doi: 10.1016/j.phytochem.2009.07.030 pmid: 19793600
[24]	Külheim C, Padovan A, Hefer C, Krause S T, Köllner T G, Myburg A A, Degenhardt J, Foley W J. The eucalyptus terpene synthase gene family. BMC Genomics, 2015, 16: 450. doi: 10.1186/s12864-015-1598-x pmid: 26062733
[25]	Whittington D A, Wise M L, Urbansky M, Coates R M, Croteau R B, Christianson D W. Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase. Proc Natl Acad Sci USA, 2002, 99: 15375-15380. doi: 10.1073/pnas.232591099
[26]	Christianson D W. Structural and chemical biology of terpenoid cyclases. Chem Rev, 2017, 117: 11570-11648. doi: 10.1021/acs.chemrev.7b00287 pmid: 28841019
[27]	Ali A, Khan M, Sharif R, Mujtaba M, Gao S J. Sugarcane omics: an update on the current status of research and crop improvement. Plants (Basel), 2019, 8: 344.
[28]	Diniz A L, Ferreira S S, Ten-Caten F, Margarido G R A, Santos J M, S Barbosa G V, Carneiro M S, Souza G M. Genomic resources for energy cane breeding in the post genomics era. Comput Struct Biotechnol, 2019, 17: 1404-1414.
[29]	Olivier G, Gaetan D, Rudie A, Jane G, Bernard P, Karen A, Jerry J, Guillaume M, Carine C, Catherine H. A mosaic monoploid reference sequence for the highly complex genome of sugarcane. Nat Commun, 2018, 9: 2638. doi: 10.1038/s41467-018-05051-5 pmid: 29980662
[30]	Zhang J, Zhang X, Tang H, Zhang Q, Hua X, Ma X, Zhu F, Jones T, Zhu X, Bowers J, Wai C, Zheng C, Shi Y, Chen S, Xu X, Yue J, Nelson D, Huang L, Li Z, Ming R. Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L. Nat Genet, 2018, 50: 1565-1573. doi: 10.1038/s41588-018-0237-2
[31]	Xu F, He L, Gao S, Su Y C, Li F, Xu L. Comparative analysis of two sugarcane ancestors Saccharum officinarum and S. spontaneum based on complete chloroplast genome sequences and photosynthetic ability in cold stress. Int J Mol Sci, 2019, 20: 3828. doi: 10.3390/ijms20153828
[32]	Souza G, Van Sluys M A, Lembke C G, Lee H, Margarido G R A, Hotta C, Gaiarsa J, Lima Diniz A, Oliveira M M, Ferreira S S, Nishiyama Jr M Y, Caten F, Ragagnin G T, Andrade P M, De Souza R F, Nicastro G, Pandya R, Kim C, Guo H, Durham A M, Carnerio M S, Zhang J S, Zhang X T, Zhang Q, Ming R, Schatz M C, Davidson B, Paterson M C, Heckerman D. Assembly of the 373k gene space of the polyploid sugarcane genome reveals reservoirs of functional diversity in the world's leading biomass crop. Gigascience, 2019, 8: giz129. doi: 10.1093/gigascience/giz129
[33]	Royer M, Pieretti I, Cociancich S, Rott P. Recent progress in understanding three major bacterial diseases of sugarcane: gumming, leaf scald and ratoon stunting. Burleigh Dodds, 2018, 26: 311-336.
[34]	李傲梅, 谭宏伟, 魏吉利, 商显坤, 黄东亮, 何为中. 草地贪夜蛾在甘蔗上的发生及防治措施. 植物保护学报, 2020, 47: 735-739.
	Li A M, Tan H W, Wei J L, Shang X K, Huang D L, He W Z. Advances in outbreak and control of fall armyworm Spodoptera frugiperda on sugarcane. J Plant Prot, 2020, 47: 735-739. (in Chinese with English abstract)
[35]	Chen C J, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant, 2020, 13: 1194-1202. doi: S1674-2052(20)30187-8 pmid: 32585190
[36]	Wang D, Tang H, Debarry J D, Tan X, Li J P, Wang X Y, Lee T H, Jin H Z, Marler B, Guo H, Kissinger J C, Paterson A H. MCScanX: a toolkit for detection and evolutionary analysis of gene synteny and collinearity. Nucleic Acids Res, 2010, 40: e49. doi: 10.1093/nar/gkr1293
[37]	Lin L H, Ntambo M S, Rott P C, Wang Q N, Lin Y H, Fu H Y, Gao S J. Molecular detection and prevalence of Xanthomonas albilineans, the causal agent of sugarcane leaf scald, in China. Crop Prot, 2018, 109: 17-23. doi: 10.1016/j.cropro.2018.02.027
[38]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 2001, 25: 402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[39]	Dudareva N, Cseke L, Blanc V M, Pichersky E. Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell, 1996, 8: 1137-1148. pmid: 8768373
[40]	Cannon S B, Mitra A, Baumgarten A, Young N D, May G. The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana. BMC Plant Biol, 2004, 4: 10. doi: 10.1186/1471-2229-4-10
[41]	Olsen J L, Rouze P, Verhelst B, Lin Y C, Bayer T, Collen J, Dattolo E, De Paoli E, Dittami S, Maumus F, Michel G, Kersting A, Lauritano C, Lohaus R, Topel M, Tonon T, Vanneste K, Amirebrahimi M, Brakel J, Bostrom C, Chovatia M, Grimwood J, Jenkins J W, Jueterbock A, Mraz A, Stam W T, Tice H, Bornberg-Bauer E, Green P J, Pearson G A, Procaccini G, Duarte C M, Schmutz J, Reusch T B, Van de Peer Y. The genome of the seagrass Zostera marina reveals angiosperm adaptation to the sea. Nature, 2016, 530: 331-335. doi: 10.1038/nature16548
[42]	Jiao Y, Liu X, Jiang H, Chen R. Research advances of plant tissue specific promoters. J Agric Sci Technol (Iran), 2019, 21: 18-28.
[43]	Aubourg S, Lecharny A, Bohlmann J. Genomic analysis of the terpenoid synthase (AtTPS) gene family of Arabidopsis thaliana. Mol Genet Genomics, 2002, 267: 730-745. pmid: 12207221
[44]	Martin D M, Aubourg S, Schouwey M B, Daviet L, Schalk M, Toub O, Lund S T, Bohlmann J. Functional annotation, genome organization and phylogeny of the grapevine (Vitis vinifera) terpene synthase gene family based on genome assembly, flcdna cloning, and enzyme assays. BMC Plant Biol, 2012, 10: 226. doi: 10.1186/1471-2229-10-226
[45]	Kumar Y, Khan F, Rastogi S. Genome-wide detection of terpene synthase genes in holy basil (Ocimum sanctum L.). PLoS One, 2018, 13: e0207097.
[46]	Liu J Y, Huang F, Wang X. Genome-wide analysis of terpene synthases in soybean: functional characterization of GmTPS3. Gene, 2014, 544: 83-92. doi: 10.1016/j.gene.2014.04.046
[47]	Chen X, Yang W, Zhang L Q. Genome-wide identification, functional and evolutionary analysis of terpene synthases in pineapple. Comp Biol Chem, 2017, 70: 40-48. doi: 10.1016/j.compbiolchem.2017.05.010
[48]	Xiong W D, Wu P Z, Jia Y X. Genome-wide analysis of the terpene synthase gene family in physic nut (Jatropha curcas L.) and functional identification of six terpene synthases. Tree Genet Genom, 2016, 12: 97. doi: 10.1007/s11295-016-1054-3
[49]	Martin H. Herbivore-induced plant volatiles: targets, perception and unanswered questions. New Phytol, 2014, 204: 297-306. doi: 10.1111/nph.12977
[50]	Jiang Z, Jacob J A, Loganathachetti D S. β-elemene: mechanistic studies on cancer cell interaction and its chemosensitization effect. Front Pharmacol, 2017, 8: 105. doi: 10.3389/fphar.2017.00105 pmid: 28337141
[51]	Paddon C J, Keasling J D. Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development. Nat Rev Microbiol, 2014, 12: 355-367. doi: 10.1038/nrmicro3240 pmid: 24686413
[52]	Cheng A X, Xiang C Y, Li J X. The rice (E)-beta-caryophyllene synthase (OsTPS3) accounts for the major inducible volatile sesquiterpenes. Phytochemistry, 2007, 68: 1632-1641. doi: 10.1016/j.phytochem.2007.04.008
[53]	Xiao Y, Wang Q, Erb M. Specific herbivore-induced volatiles defend plants and determine insect community composition in the field. Ecol Lett, 2012, 15: 1130-1139. doi: 10.1111/j.1461-0248.2012.01835.x pmid: 22804824
[54]	Chen X, Chen H, Yuan J S, Köllner T G, Chen Y, Guo Y, Zhuang X, Chen X, Zhang Y, Fu J, Nebenführ A, Guo Z, Chen F. The rice terpene synthase gene OsTPS19 functions as an (S)-limonene synthase in planta and its overexpression leads to enhanced resistance to the blast fungus Magnaporthe oryzae. Plant Biotechnol J, 2018, 16: 1778-1787. doi: 10.1111/pbi.12914
[55]	Rodríguez A, Andrés V S, Cervera M, Redondo A, Leandro P. The monoterpene limonene in orange peels attracts pests and microorganisms. Plant Signal Behav, 2011, 6: 1820-1823. doi: 10.4161/psb.6.11.16980 pmid: 22212123
[56]	Huang M, Sanchez-Moreiras A M, Abel C. Sohrabi R, Lee S, Gershenzon J, Tholl D. The major volatile organic compound emitted from Arabidopsis thaliana flowers, the sesquiterpene (E)-β-caryophyllene, is a defense against a bacterial pathogen. New Phytol, 2012, 193: 997-1008. doi: 10.1111/j.1469-8137.2011.04001.x
[57]	Chiriboga M X, Campos-Herrera R, Jaffuel G, RóDer G, Turlings T C J. Diffusion of the maize root signal (E)-β-caryophyllene in soils of different textures and the effects on the migration of the entomopathogenic nematode Heterorhabditis megidis. Rhizosphere, 2017, 3: 53-59. doi: 10.1016/j.rhisph.2016.12.006
[58]	Ding Y, Huffaker A, KöLlner T G, Weckwerth P, Robert C A M, Spencer J L, Lipka A E, Schmelz E A. Selinene volatiles are essential precursors for maize defense promoting fungal pathogen resistance. Plant Physiol, 2017, 175: 1455-1468. doi: 10.1104/pp.17.00879 pmid: 28931629
[59]	卢凯, 李欣, 周嘉良, 解晓军, 戚舒, 周强. 虫害诱导的水稻挥发物抑制水稻病原菌的生长. 科学通报, 2010, 55: 2927-2932.
	Lu K, Li X, Zhou J L, Xie X J, Qi S, Zhou Q. Insect-induced rice volatiles inhibit the growth of rice pathogenic bacteria. Sci Bull, 2010, 55: 2927-2932. (in Chinese with English abstract)
[60]	刘小香, 陈秋波, 王真辉, 谢龙莲, 徐志. 巨尾桉挥发油对真菌和昆虫的化感作用. 生态学杂志, 2007, 26: 835-839.
	Liu X X, Chen Q B, Wang Z H, Xie L L, Xu Z. Allelopathy of volatile oil from Eucalyptus grandis on fungi and insects. Chin J Ecol, 2007, 26: 835-839. (in Chinese with English abstract)

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基因名称 Gene name	正向引物 Forward primer (5'-3')	反向引物 Reverse primer (5'-3')
SsTPS5	AGTACCGCATGCCCTACCTA	CACCGCTTCTTGCTGAAACC
SsTPS6	AGTACCGCATGCCCTACCTA	CGCTGCAAGTGTTCCTCAAC
SsTPS8	ATGATGTTCAGGGGCTGCAA	CATGTATGCACGCTTGGGTG
SsTPS10	AGGGGAAAGTGCGTCAGATG	AGTGTGGATACGGCTTAGCG
SsTPS15	AAAAGACCGGACCTTCCACC	CATGTATGCACGCTTGGGTG
SsTPS18	ACCAAGGAGGCATTCGAGTG	CGCGTTCTTCCTCCCTTTCT
SsTPS26	GTCCCATGCACTCCATCACA	GAGGTGGCAACGGTTCCTAA
SsTPS27	AATGGGCTCTCACCTTTCCG	AAGCTTGTTCACGCTCATGC
SsTPS35	TGTTCCTAGGCCATGCAAGG	TTTTGGACTCCGAGTGGCTC
SsTPS36	TGCTGCTGCGCAATGATTTT	GGTCGCTCTTCTGACGTTGA
SsTPS37	GAGCCAAGCCTTGCAGAGTA	ATGGGCAGGCTCTGTAGGTA
SsTPS39	GAAATACCTGTGGTGCGGGA	CCACAACATAGACAAGCGCG
GAPDH	CACGGCCACTGGAAGCA	TCCTCAGGGTTCCTGATGCC

Genome-wide analysis of terpene synthase (TPS) gene family and its expression under biological stress in Saccharum spontaneum

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