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Acta Agronomica Sinica ›› 2023, Vol. 49 ›› Issue (6): 1715-1725.doi: 10.3724/SP.J.1006.2023.24142

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Genome-wide identification and expression analysis of PHB gene family in sweet potato

MEI Yu-Qin1,2,**(), LIU Yi1,**(), WANG Chong2,3, LEI Jian2, ZHU Guo-Peng1,*(), YANG Xin-Sun2,*()   

  1. 1Horticulture College, Hainan University/Key Laboratory for Quality Regulation of Tropical Horticultural Plants of Hainan Province, Haikou 570228, Hainan, China
    2Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China
    3College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
  • Received:2022-06-16 Accepted:2022-10-11 Online:2023-06-12 Published:2022-10-21
  • Contact: *E-mail: guopengzhu@163.com;E-mail: yangxins013@163.com
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    China Agriculture Research System of MOF and MARA(CARS-10-C13-2021);National Key Research and Development Program of China “Creation of Resistant Germplasm for Dicotyledonous Grains”(2019YFD1001303-4);Leading Talent Training Plan of Hubei Academy of Agricultural Sciences(L2018005);Hubei Province Key Research and Development Program Project “China and Benin Eshu Series New Varieties and New Technology Demonstration”(2020BHB024)

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Abstract:

The prohibitins (PHB) are SPFH domain-containing proteins found in the prokaryotes to eukaryotes. Plant PHB gene family is involved in many important functions in a variety of different biological processes, including growth and development and responses to biotic and abiotic stresses. At present, PHB proteins have been identified in Arabidopsis, rice, maize, soybean, tomato, upland cotton, and other plants. However, the systematic analysis of PHB family in sweet potato is still uncertain. In this study, 11 PHB genes in sweet potato were identified, and the analysis of these conserved protein motifs and gene structures showed that they were highly conserved in phylogenetic subgroups. In addition, the cis-acting elements related to various hormone regulation and stress were predicted in the promoter region and the relative expression patterns of IbPHB genes were different in different parts of plants and under different abiotic stresses. This study systematically analyzed the general characteristics of IbPHB gene in sweet potato, and provided a theoretical basis for the study of functional characteristics of PHB gene in sweet potato and other plants.

Key words: sweet potato, PHB gene family, phylogenetic evolution, bioinformatics, the relative expression level

Table 1

Primers used in this study"

引物用途
Primer function		 引物名称
Primer name		 引物序列Primer sequence
正向引物序列
Forward sequence (5°-3°)		 反向引物序列
Reverse sequence (5°-3°)
qRT-PCR 扩增
The qRT-PCR
amplification		 IbPHB1 CACGTGTCTGCTTTGGTTCG TGCTGAGCAACCTGCTTTTG
IbPHB2 CTTTGACATTCGCACCAGGC GCACCCGGAGAGTGAGATTC
IbPHB3 GTGGAGAAATGGGGTCGGTT ACGTCCAGAGAGCAGATCCT
IbPHB4 CCACCGCTTCTTCTCCCATT AGTCATGCCTGGAGCTAGGA
IbPHB5 GTCTTCGACGTTTCTCCGGT TGGCGTACTTCAAGAGGCTG
IbPHB6 TCTGGCGAAGAAGAAGGCTG CTCTGCATCTCTAAGCGCCA
IbPHB7 TGCTGCTGCAAGACTTAGGG TTTGATTCCGCTTCCCCCTC
IbPHB8 AGTATAACGCCAGCCAGCTC GTTGAAGTTAGCCGCCCTCT
IbPHB9 GGTGACTCCTTCGCTGTCAA CCTCCTCTCCAGTAGACCCC
IbPHB10 GGCAAGCTGCGAATGAGAAG CGCGAGGTACTTGGACTCTG
IbPHB11 ATGAAGGAAATTGGCGCTGC GATGGTCAGACTGGGAAGCC
内参基因
Internal reference genes		 β-Actin AGCAGCATGAAGATTAAGGTTGTAGCAC TGGAAAATTAGAAGCACTTCCTGTGAAC

Table 2

PHB gene family in sweet potato"

基因名称 Gene name 基因编号Gene ID 染色体定位
Chromosome
location		 氨基酸
长度
Amino acid length		 等电点Isoelectric
point		 相对分子量Molecular
weight (kD)		 不稳定指数Instability index 脂溶系数 Aliphatic index 亲水性
平均值
Grand average of
hydropathicity		 亚细胞定位
Subcellular
localization
IbPHB1 g1330.t1 LG1(+): 8004568-8006394 277 6.55 30,473.85 40.46 98.59 -0.072 线粒体Mitochondrion
IbPHB2 g1344.t1 LG1 (-): 8093622-8097050 276 8.77 30,402.86 45.33 98.95 -0.106 线粒体Mitochondrion
IbPHB3 g1611.t1 LG1 (+): 10011048-10014530 314 5.38 34,754.86 30.67 91.59 -0.129 线粒体Mitochondrion
基因名称 Gene name 基因编号Gene ID 染色体定位
Chromosome
location		 氨基酸
长度
Amino acid length		 等电点Isoelectric
point		 相对分子量Molecular
weight (kD)		 不稳定指数Instability index 脂溶系数 Aliphatic index 亲水性
平均值
Grand average of
hydropathicity		 亚细胞定位
Subcellular
localization
IbPHB4 g20708.t1 LG5 (+): 29636330-29640330 400 8.89 43,335.23 46.92 90.35 -0.314 线粒体Mitochondrion
细胞核Nucleus
IbPHB5 g22098.t1 LG6 (-): 9204364-9206354 474 5.57 52,238.60 26.20 89.01 -0.343 细胞核Nucleus
IbPHB6 g22144.t1 LG6 (+): 9498264-9500186 446 5.40 49,012.72 27.04 86.95 -0.388 细胞核Nucleus
IbPHB7 g30111.t1 LG7 (+): 34852091-34854904 286 5.30 31,314.79 37.48 93.11 -0.115 线粒体Mitochondrion
IbPHB8 g30789.t1 LG8 (+): 2906392-2910014 283 9.23 31,632.31 36.05 101.38 -0.113 线粒体Mitochondrion
IbPHB9 g47367.t1 LG12 (+): 3383794-3389526 313 6.57 35,302.44 38.84 82.62 -0.401 细胞质Cytoplasm
线粒体Mitochondrion
细胞核Nucleus
IbPHB10 g53565.t1 LG13 (-): 17737704-17740759 287 6.26 31,728.35 23.91 94.81 -0.197 线粒体Mitochondrion
IbPHB11 g59949.t1 LG15 (+): 555231-558804 277 5.84 30,569.89 26.37 93.65 -0.200 线粒体Mitochondrion

Fig. 1

Phylogenetic tree of PHB gene family proteins in sweet potato and Arabidopsis thaliana"

Fig. 2

Distribution of PHB gene family on chromosomes in sweet potato"

Fig. 3

Phylogenetic tree and gene structure of PHB gene family in sweet potato A: the phylogenetic tree of PHB family genes in sweet potato; B: IbPHB conservative motif; C: the distribution of conservative domains."

Fig. 4

Promoter prediction of PHB gene family in sweet potato"

Fig. 5

Tissue specific expression analysis of IbPHB gene family Different lowercase letters in the figure indicate significant differences between the data at the 0.05 probability level."

Fig. 6

Relative expression levels of IbPHB gene family in response to high salt stress Different lowercase letters in the figure indicate significant differences between the data at the 0.05 probability level."

Fig. 7

Relative expression levels of IbPHB gene family in response to drought stress Different lowercase letters in the figure indicate significant differences between the data at the 0.05 probability level."

[1] Duncan T B, Maja B H, Stephen M R. The SPFH domain- containing proteins: more than lipid raft markers. Trends Cell Biol, 2007, 17: 394-402.
doi: 10.1016/j.tcb.2007.06.005
[2] Morrow I C, Parton R G. Flotillins and the PHB domain protein family: rafts, worms and anaesthetics. Traffic, 2005, 6: 725-740.
doi: 10.1111/j.1600-0854.2005.00318.x pmid: 16101677
[3] Di C, Xu W Y, Su Z, Yuan J. Comparative genome analysis of PHB gene family reveals deep evolutionary origins and diverse gene function. BMC Bioinf, 2010, 11: S22.
[4] McClung J K, Danner D B, Stewart D A, Smith J R, Schneider E L, Lumpkin C K, Dell’Orco R T, Nuell M J. Isolation of a cDNA that hybrid selects antiproliferative mRNA from rat liver. Biochem Biophys Res Commun, 1989, 164: 1316-1322.
doi: 10.1016/0006-291X(89)91813-5
[5] Sato T, Sakamoto T, Takita K, Saito H, Okui K, Nakamura Y. The human prohibitin (PHB) gene family and its somatic mutations in human tumors. Genomics, 1993, 17: 762-764.
doi: 10.1006/geno.1993.1402 pmid: 8244394
[6] 黄浩浩. PHB基因对胶质瘤干细胞的调控研究. 中国人民解放军军事医学科学院硕士学位论文, 北京, 2017.
Huang H H. The Roles of PHB on the Regulation of Glioma Stem Cell. MS Thesis of PLA Academy of Military Medical Sciences, Beijing, China, 2017. (in Chinese with English abstract)
[7] Wu Y W, Yang S H, Hwangbo M, Chu K H. Analysis of Zobellella denitrificans ZD1 draft genome: genes and gene clusters responsible for high polyhydroxybutyrate (PHB) production from glycerol under saline conditions and its CRISPR-Cas system. PLoS One, 2019, 14: e0222143.
[8] Van Aken O, Pecenková T, Van De Cotte B, De Rycke R, Eeckhout D, Fromm H, De Jaeger G, Witters E, Beemster G T S, Inzé D, Van Breusegem F. Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development. Plant J, 2007, 52: 850-864.
doi: 10.1111/tpj.2007.52.issue-5
[9] Ahn C S, Lee J H, Reum H A, Kim W T, Pai H S. Prohibitin is involved in mitochondrial biogenesis in plants. Plant J, 2006, 46: 658-667.
doi: 10.1111/j.1365-313X.2006.02726.x pmid: 16640602
[10] Christians M J, Larsen P B. Mutational loss of the prohibitin AtPHB3 results in an extreme constitutive ethylene response phenotype coupled with partial loss of ethylene-inducible gene expression in Arabidopsis seedlings. J Exp Bot, 2007, 58: 2237-2248.
doi: 10.1093/jxb/erm086 pmid: 17525078
[11] 王晓萍, Amber R, Kati W, Albert Y, Nigel M C, 王勇. 拟南芥抑制蛋白基因PHB3在NO介导反应和H2O2诱导NO积累过程中的功能研究. 见:诸葛健, 李华钟主编. 山东植物生理学会第七次代表大会暨植物生物学与现代农业研讨会论文集. 山东泰安: 山东省科学技术协会, 2012. pp 51-61.
Wang X P, Amber R, Kati W, Albert Y, Nigel M C, Wang Y. The function of Arabidopsis inhibitor protein gene PHB3 in NO mediated reaction and H2O2 induced NO accumulation. In: Zhu G J, Li H Z, eds. Shandong Plant Physiology Association Seventh Congress and Workshop on Plant Biology and Modern Agriculture. Tai’an, Shandong, China: Shandong Science and Technology Association, 2012. pp 51-61. (in Chinese)
[12] Song M, Peng X Y, Du C F, Lei L, Zhang T, Xiang Y. Genome-wide analysis of the PHB gene family in Glycine max (L.) Merr. Genes Genomics, 2017, 39: 1095-1106.
doi: 10.1007/s13258-017-0580-1
[13] Huang F Y, Ye X W, Wang Z J, Ding Y, Cai X J, Yu L, Waseem M, Abbas F, Ashraf U, Chen X L, Ke Y G. The prohibitins (PHB) gene family in tomato: Bioinformatic identification and expression analysis under abiotic and phytohormone stresses. GM Crops Food, 2021, 12: 535-550.
doi: 10.1080/21645698.2021.1872333
[14] 章妮, 陈克龙. 陆地棉PHB基因家族鉴定与表达分析. 分子植物育种, 2021. https://kns.cnki.net/kcms/detail/46.1068.S.20211216.1051.007.html.
Zhang N, Chen K L. Identification and expression analysis of PHB gene family in upland cotton. Mol Plant Breed, 2021, https://kns.cnki.net/kcms/detail/46.1068.S.20211216.1051.007.html. (in Chinese with English abstract)
[15] Wen X J, Niu T T, Kong X P. In silico analysis of PHB gene family in maize. Plant Growth Regul, 2014, 73: 181-191.
doi: 10.1007/s10725-013-9879-3
[16] 王欣, 李强, 曹清河, 马代夫. 中国甘薯产业和种业发展现状与未来展望. 中国农业科学, 2021, 54: 483-492.
doi: 10.3864/j.issn.0578-1752.2021.03.003
Wang X, Li Q, Cao Q H, Ma D F. Current status and future prospective of sweetpotato production and seed industry in China. Sci Agric Sin, 2021, 54: 483-492 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2021.03.003
[17] Yang J, Moeinzadeh M H, Kuhl H, Helmuth J, Xiao P, Haas S, Liu G L, Zheng J L, Sun Z, Fan W J, Deng G F, Wang H X, Hu F H, Zhao S S, Fernie A R, Boerno S, Timmermann B, Zhang P, Vingron M. Haplotype-resolved sweet potato genome traces back its hexaploidization history. Nat Plants, 2017, 3: 696-703.
doi: 10.1038/s41477-017-0002-z pmid: 28827752
[18] 刘振雷, 潘家荃, 周桦楠, 刘冠求, 于涛. 甘薯耐冷组学及分子生物学研究进展. 江苏农业科学, 2021, 49(24): 45-48.
Liu Z L, Pan J Q, Zhou H N, Liu G Q, Yu T. Research progress on cold tolerance omics and molecular biology of sweet potato. Jiangsu Agric Sci, 2021, 49(24): 45-48. (in Chinese with English abstract)
[19] 陈璐, 周淑倩, 李永新, 陈刚, 陆国权, 杨虎清. 甘薯解偶联蛋白基因家族鉴定与表达分析. 作物学报, 2022, 48: 1683-1696.
doi: 10.3724/SP.J.1006.2022.14126
Chen L, Zhou S Q, Li Y X, Chen G, Lu G Q, Yang H Q. Identification and expression analysis of uncoupling protein gene family in sweet potato. Acta Agron Sin, 2022, 48: 1683-1696. (in Chinese with English abstract)
[20] 饶莉萍. 甘薯IbCCoAOMT基因的克隆与功能鉴定. 长江大学硕士学位论文, 湖北荆州, 2021.
Rao L P. Cloning and Functional Identification of IbCCoAOMT Gene from Ipomoea batatas (L.) Lam. MS Thesis of Yangtze University, Jingzhou, Hubei, China, 2021. (in Chinese with English abstract)
[21] 梁璇, 李鹏, 杨哲, 贾小云, 王文斌. 甘薯近缘二倍体野生种TPS家族全基因组鉴定及表达分析. 山西农业科学, 2022, 50: 605-612.
Liang X, Li P, Yang Z, Jia X Y, Wang W B. Identification and expression analysis of genome of TPS family of wild relative diploid species of sweet potato. Shanxi Agric Sci, 2022, 50: 605-612. (in Chinese with English abstract)
[22] 张文杰, 辛曙丽, 黄哲瑞, 祝志欣, 司成成, 陈艳丽, 朱国鹏, 刘永华. 甘薯块根发育过程中关键蔗糖分解酶及其基因家族成员的鉴定. 热带作物学报, 2022, 43: 1535-1544.
Zhang W J, Xin S L, Huang Z R, Zhu Z X, Si C C, Chen Y L, Zhu G P, Liu Y H. Identification of key sucrose-degrading enzymes and the relevant gene family members in the development of sweetpotato storage roots. Chin J Trop Crops, 2022, 43: 1535-1544. (in Chinese with English abstract)
[23] 黄小芳, 毕楚韵, 陈其俊, 刘江洪, 胡韵卓, 黄碧芳, 陈选阳, 林世强. 甘薯α-淀粉酶基因的全基因组鉴定和分析. 分子植物育种, 2022, 20: 8035-8042.
Huang X F, Bi C Y, Chen Q J, Liu J H, Hu Y Z, Huang B F, Chen X Y, Lin S Q. Genome-wide identification and analysis of α- amylase gene family of Ipomoea batatas. Mol Plant Breed, 2022, 20: 8035-8042. (in Chinese with English abstract)
[24] 黄小芳, 毕楚韵, 黄伟群, 刘江洪, 胡韵卓, 黄碧芳, 林世强, 陈选阳. 甘薯β-淀粉酶家族基因的全基因组鉴定和表达分析. 华南农业大学学报, 2021, 42(5): 50-59.
Huang X F, Bi C Y, Huang W Q, Liu J H, Hu Y Z, Huang B F, Lin S Q, Chen X Y. Genome-wide identification and expression analysis of the β-amylase gene family in Ipomoea batatas. J South China Agric Univ, 2021, 42(5): 50-59. (in Chinese with English abstract)
[25] 黄小芳, 毕楚韵, 石媛媛, 胡韵卓, 周丽香, 梁才晓, 黄碧芳, 许明, 林世强, 陈选阳. 甘薯基因组NBS-LRR类抗病家族基因挖掘与分析. 作物学报, 2020, 46: 1195-1207.
doi: 10.3724/SP.J.1006.2020.94163
Huang X F, Bi C Y, Shi Y Y, Hu Y Z, Zhou L X, Liang C X, Huang B F, Xu M, Lin S Q, Chen X Y. Discovery and analysis of NBS-LRR gene family in sweet potato genome. Acta Agron Sin, 2020, 46: 1195-1207. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2020.94163
[26] 许光龄, 王建伟, 陈玥, 陈艳丽, 祝志欣, 朱国鹏. 甘薯NRT基因家族成员鉴定及其在不同氮素条件下的表达模式分析. 分子植物育种, 2022, 20: 7316-7331.
Xu G L, Wang J W, Chen Y, Chen Y L, Zhu Z X, Zhu G P. Identification of sweetpotato NRT gene family members and expression patterns analysis under different nitrogen conditions. Mol Plant Breed, 2022, 20: 7316-7331 (in Chinese with English abstract).
[27] 毕楚韵, 黄小芳, 王和寿, 陈其俊, 胡韵卓, 黄碧芳, 许明, 杨志坚, 林世强, 陈选阳. 甘薯全基因组WRKY转录因子的基因鉴定与逆境胁迫表达分析. 西北农林科技大学学报(自然科学版), 2021, 49(9): 30-44.
Bi C Y, Huang X F, Wang H S, Chen Q J, Hu Y Z, Huang B F, Xu M, Yang Z J, Lin S Q, Chen X Y. Identification of WRKY transcription factor genes in Ipomoea batatas genome and expression analysis under stresses. J Northwest A&F Univ (Nat Sci Edn), 2021, 49(9): 30-44. (in Chinese with English abstract)
[28] 黄小芳, 毕楚韵, 王和寿, 陈其俊, 胡韵卓, 陈选阳, 林世强. 甘薯NAC转录因子家族的全基因组鉴定与分析. 热带作物学报, 2021, 42: 1831-1840.
Huang X F, Bi C Y, Wang H S, Chen Q J, Hu Y Z, Chen X Y, Lin S Q. Genome-wide identification and analysis of NAC transcription factor family of Ipomoea batatas. Chin J Trop Crops, 2021, 42: 1831-1840. (in Chinese with English abstract)
[29] Zhang H, Gao X R, Zhi Y H, Li X, Zhang Q, Niu J B, Wang J, Zhai H, Zhao N, Li J G, Liu Q C, He S Z. A non-tandem CCCH-type zinc-finger protein, IbC3H18, functions as a nuclear transcriptional activator and enhances abiotic stress tolerance in sweet potato. New Phytol, 2019, 223: 1918-1936.
doi: 10.1111/nph.15925 pmid: 31091337
[30] 宋小锋, 原增艳. 甘薯水通道蛋白基因家族的全基因组鉴定和表达分析. 分子植物育种, 2022, 20: 1452-1461.
Song X F, Yuan Z Y. Genome-wide identification and expression pattern profiling of aquaporin gene family in Ipomoea batatas. Mol Plant Breed, 2022, 20: 1452-1461. (in Chinese with English abstract)
[31] 宋天晓, 刘意, 饶莉萍, Soviguidi D R J, 朱国鹏, 杨新笋. 甘薯细胞壁蔗糖转化酶基因IbCWIN家族成员鉴定及表达分析. 作物学报, 2021, 47: 1297-1308.
doi: 10.3724/SP.J.1006.2021.04180
Song T X, Liu Y, Rao L P, Soviguidi D R J, Zhu G P, Yang X S. Identification and expression analysis of cell wall invertase IbCWIN gene family members in sweet potato. Acta Agron Sin, 2021, 47: 1297-1308. (in Chinese with English abstract)
[32] 周淑倩, 陈璐, 陈惠云, 李永新, 陈刚, 陆国权, 杨虎清. 甘薯交替氧化酶基因家族生物信息学与表达分析. 核农学报, 2022, 36: 270-281.
doi: 10.11869/j.issn.100-8551.2022.02.0270
Zhou S Q, Chen L, Chen H Y, Li Y X, Chen G, Lu G Q, Yang H Q. Bioinformatics and expression analysis of alternative oxidase genes in sweetpotato. J Nucl Agric Sci, 2022, 36: 270-281. (in Chinese with English abstract)
doi: 10.11869/j.issn.100-8551.2022.02.0270
[33] 毕楚韵, 黄小芳, 黄伟群, 刘江洪, 胡韵卓, 黄碧芳, 林世强, 陈选阳. 甘薯基因组BBX转录因子基因鉴定与逆境胁迫表达分析. 四川农业大学学报, 2021, 39(2): 157-165.
Bi C Y, Huang X F, Huang W Q, Liu J H, Hu Y Z, Huang B F, Lin S Q, Chen X Y. Identification of BBX transcription factor genes in Ipomoea batatas genome and expression analysis under stresses. J Sichuan Agric Univ, 2021, 39(2): 157-165. (in Chinese with English abstract)
[34] 邵正伟, 曾志鹏, 陈彦竹, 何敏红, 张毅, 陈善兰, 朱宏波. 甘薯全基因组SBP-box基因家族鉴定及表达分析. 分子植物育种, 2021. https://kns.cnki.net/kcms/detail/46.1068.S.20210722.1407.014.html.
Shao Z W, Zeng Z P, Chen Y Z, He M H, Zhang Y, Chen S L, Zhu H B. Genome-wide identification and expression analysis of the SBP-box gene family in sweet potato (Ipomoea batatas). Mol Plant Breed, 2021. https://kns.cnki.net/kcms/detail/46.1068.S.20210722.1407.014.html. (in Chinese with English abstract)
[35] 夏梦, 徐畅, 王吉, 王玲, 孙丹. 植物Prohibitins基因(PHBs)的研究进展. 中国农学通报, 2016, 32(35): 24-29.
doi: 10.11924/j.issn.1000-6850.casb16060159
Xia M, Xu C, Wang J, Wang L, Sun D. Research progress of plant Prohibitins gene (PHBs). Chin Agric Sci Bull, 2016, 32(35): 24-29. (in Chinese with English abstract)
doi: 10.11924/j.issn.1000-6850.casb16060159
[36] 熊炤成, 屈春凤, 沈开元, 刘庆友, 王英群, 石德顺, 李湘萍. 水牛PHB基因克隆与表达模式. 中国兽医学报, 2016, 36: 1422-1428.
Xiong Z C, Qu C F, Shen K Y, Liu Q Y, Wang Y Q, Shi D Y, Li X P. Cloning and expression pattern of buffalo PHB gene. Chin J Veterin Sci, 2016, 36: 1422-1428. (in Chinese with English abstract)
[37] 王晗, 闫敏, 许晔, 王仁雷, 刘少华. 芸薹属物种(B. napus, B. rapa, B. oleracea) PHB基因的生物信息学分析. 分子植物育种, 2021, 19: 4232-4241.
Wang H, Yan M, Xu Y, Wang R L, Liu S H. Bioinformatics analysis of PHB gene in Brassica species (B. napus, B. rapa, B. oleracea). Mol Plant Breed, 2021, 19: 4232-4241. (in Chinese with English abstract)
[38] 张佳宇. 黄瓜CsPHB基因功能的初步分析. 西北农林科技大学硕士学位论文,陕西杨凌, 2020.
Zhang J Y. A Preliminary Analysis of the Function of the CsPHB Gene in Cucumber (Cucumis sativus L.). MS Thesis of Northwest A&F University, Yangling, Shaanxi, China, 2020. (in Chinese with English abstract)
[39] 徐丽. 利用基因工程进行PHB相关基因的克隆和烟草转化. 山东师范大学硕士学位论文, 山东济南, 2000.
Xu L. Cloning and Transformation of PHB Related Genes in Tobacco by Genetic Engineering. MS Thesis of Shandong Normal University, Jinan, Shandong, China, 2000. (in Chinese with English abstract)
[40] 张瑜. 条斑紫菜PHB基因克隆及生物信息学分析. 辽宁师范大学硕士学位论文, 辽宁大连, 2014.
Zhang Y. Molecular Cloning and Bioinformatics Analysis of Prohibitin from Porphra yezoensis. MS Thesis of Liaoning Normal University, Dalian, Liaoning, China, 2014. (in Chinese with English abstract)
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